Abstract
Continuous culture experiments with identical experimental designs were run with a mixed microbial community of activated sludge origin and an axenic bacterial culture derived from it. Each culture received 2-chlorophenol (2-CP) at a concentration of 160 mg/L as COD and L-lysine at a concentration of 65 mg/L as COD. A factorial experimental design was employed with dilution rate and media composition as the two controlled variables. Three dilution rates were studied: 0.015, 0.0325, and 0.05 h−1. Media composition was changed by adding four biogenic compounds (butyric acid, thymine, glutamic acid and lactose) in equal COD proportions at total concentrations of 0, 34, 225, and 1462 mg/L as COD. The measured variables were the effluent concentrations of 2-CP as measured by the 4-aminoantipyrene test and lysine as measured by the o-diacetylbenzene procedure. The results suggest that community structure and substrate composition play important roles in the response of a microbial community to mixed substrates. The addition of more biogenic substrates to the axenic culture had a deleterious effect on the removal of both lysine and 2-CP, although the effect was much larger on lysine removal. In contrast, additional substrates had a positive effect on the removal of 2-CP by the mixed community and much less of a negative effect on the removal of lysine. The dilution rate at which the cultures were growing had relatively little impact on the responses to the additional substrates.
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Abbreviations
- COD:
-
chemical oxygen demand
- 2-CP:
-
2-chlorophenol
- DOC:
-
dissolved organic carbon
- MDL:
-
method detection limit
- SS:
-
suspended solids
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Leslie Grady, C.P., Cordone, L. & Cusack, L. Effects of media composition on substrate removal by pure and mixed bacterial cultures. Biodegradation 4, 23–38 (1993). https://doi.org/10.1007/BF00701452
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DOI: https://doi.org/10.1007/BF00701452