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Molecular differentiation of Keratinomyces (Trichophyton) species

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Abstract

The taxonomy of the form-genus Keratinomyces (Trichophyton) within the group of the dermatophytes is based on morphological features which remain insufficient for the distinction of these anamorphic species. The three species included in the genus Keratinomyces, namely K. ajelloi, K. ceretanicus and K. longifusus were examined by means of their mitochondrial-like DNA diversity and compared to few other dermatophytes. The analysis of the mtDNA restriction fragments confirmed that the three species are different and well separate from the other dermatophytes.

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References

  1. Ajello, L (1968) A taxonomic review of the dermatophytes and related species. Sabouraudia 6: 147–159

    PubMed  Google Scholar 

  2. Ajello, L (1974) Natural history of the dermatophytes and related fungi. Mycopathol. Mycol. Appl. 53: 93–110

    PubMed  Google Scholar 

  3. Anderson, JB, Petsche, DM & Smith, ML (1987) Restriction fragment length polymorphisms in biological species of Armillaria mellea. Mycologia 79: 69–76

    Google Scholar 

  4. Arx von, JA (1987) A re-evaluation of the Eurotiales. Persoonia 13: 273–300

    Google Scholar 

  5. Bates, MR, Buck, KW & Brasier, CM (1993) Molecular relationships between Ophiostoma ulmi and the NAN and EAN races of O. novo-ulmi determined by restriction fragment length polymorphisms of nuclear DNA. Mycol. Res. 97: 449–455

    Google Scholar 

  6. Bruns, TD, Palmer, JD, Shumard, DS & Hudspeth, MES (1988) Mitochondrial DNAs of Suillus: Three fold size changes in molecules that share a common gene order. Exp. Mycol. 15: 316–325

    Google Scholar 

  7. Bruns, TD, White, TJ & Taylor, JW (1991) Fungal molecular systematics. Annu. Rev. Ecol. Syst. 22: 525–564

    Google Scholar 

  8. Cano, J & Sigler, L (1992) Re-evaluation of the synonymy between Keratinomyces ceretanicus and Trichophyton ajelloi. J. Med. Vet. Mycol. 30: 327–331

    PubMed  Google Scholar 

  9. Crous, PW, Janse, BJH, Victor, D, Marais, GF & Alfenas, AC (1993) Characterization of some Cylindrocladium species with three-septate conidia using morphology, isoenzyme banding patterns and DNA polymorphisms. System. Appl. Microbiol. 16: 266–273

    Google Scholar 

  10. Estruch, JJ, Antuña, C, Ferrer, S & Ramón, D (1989) Aislamiento de DNA genómico de Trichophyton mentagrophytes. Rev. Iber. Micol. 6: 62–66

    Google Scholar 

  11. Förster, H, Kinshcerf, TG, Leong, SA & Maxwell, DP (1989) Restriction fragment length polymorphisms of the mitochondrial DNA of Phytophthora megasperma isolated from soybean, alfalfa and fruit trees, Can. J. Bot. 67: 529–537

    Google Scholar 

  12. Guillamón, J, Barrio, E, Huerta, T & Querol, A (1994) Rapid characterization of four species of the Saccharomyces sensu stricto complex according to mitochondrial DNA patterns. Int. J. Syst. Bacteriol. 44: 708–714

    PubMed  Google Scholar 

  13. Houben, P & Clark-Walker, GD (1986) An approach to yeast classification by mapping mitochondrial DNA from Dekkera/Brettanomyces and Einiella genera. Curr. Gen. 8: 621–628

    Google Scholar 

  14. Kawasaki, M, Ishizaki, H, Aoki, M & Watanabe, S (1990) Phylogeny of Nannizzia incurvata, Nannizzia gypsea, Nannizzia fulva and Nanizzia otae by restriction enzyme analysis of mitochondrial DNA. Mycopathologia. 112: 95–102

    Google Scholar 

  15. Nishio, T, Kawasaki, M & Ishizaki, H (1992) Phylogeny of the genera Trichophyton using mitochondrial DNA analysis. Mycopathologia. 117: 127–132

    PubMed  Google Scholar 

  16. Padhye, AA, Immwidthaya, S, Jeffries, CD & Ajello, L (1987) Mating behaviour of Keratinomyces ceretanicus with Arthroderma uncinatum. J. Med. Vet. Mycol. 25: 195–201

    PubMed  Google Scholar 

  17. Punsola, L & Guarro, J (1984) Keratinomyces ceretanicus sp. nov., a psychrophilic dermatophyte from soil. Mycopathologia 85: 185–190

    PubMed  Google Scholar 

  18. Querol, A, Barrio, E & Ramón, D (1992) A comparative study of different methods of yeast strain characterization. System. Appl. Microbiol. 15: 439–446

    Google Scholar 

  19. Sambrook, J, Fritsch, EF & Maniatis, T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  20. Thomas, V, Rutherford, MA & Bridge, PD (1994) Molecular differentiation of two races of Fusarium oxysporum special form cubense. Lett. Appl. Microbiol. 18: 193–196

    Google Scholar 

  21. Tortajada, C, Ferrer, S & Ramón, D (1993) Nucleotide sequence of a Trichophyton mentagrophytes HindIII mitochondrial DNA fragment containing a tRNA gene cluster. FEMS Microbiol. Lett. 109: 151–158

    PubMed  Google Scholar 

  22. Vanbreuseghem, R (1952) Intérêt théorique et pratique d'un nouveau dermatophyte isolé du sol: Keratinomyces ajelloi, gen. nov. sp. nov. Bull. Ac. Roy. de Belgique (Classe des Sciences) séance du 8 nov. 38: 1068–1077

    Google Scholar 

  23. Vanbreuseghem, R (1981) Keratinomyces ajelloi ou Trichophyton ajelloi?. Bull. Soc. Fr. Mycol. Méd 10: 257–260

    Google Scholar 

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Authors and Affiliations

  1. Unidad de Microbiología Escuela de Enología, Universidad Rovira i Virgili, Ramón y Cajal s/n, 43005, Tarragona, Spain

    J. M. Guillamón & J. Cano

  2. Unidad de Microbiología, Facultad de Medicina, Universidad Rovira i Virgili, San Lorenzo 21, 43201, Reus, Spain

    J. Guarro

  3. Departamento de Biotecnología de los Alimentos, Instituto de Agroquímica y Tecnología de los Alimentos (C.S.I.C.), Jaime Roig 11, 46010, Valencia, Spain

    D. Ramón

Authors
  1. J. M. Guillamón
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  2. J. Cano
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  3. D. Ramón
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  4. J. Guarro
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Guillamón, J.M., Cano, J., Ramón, D. et al. Molecular differentiation of Keratinomyces (Trichophyton) species. Antonie van Leeuwenhoek 69, 223–227 (1996). https://doi.org/10.1007/BF00399610

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  • DOI: https://doi.org/10.1007/BF00399610

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