Abstract
A total of 21 clinical isolates of Campylobacter pylori from Peru and the United Kingdom and two reference strains (from Australia), including the type strain (NCTC 11637T), were characterized by high resolution one-dimensional SDS-polyacrylamide gel electrophoresis of cellular proteins. The protein patterns contained more than 40 discrete bands and the approximate molecular weights of the major bands were 22, 27, 46, 57, 60, 65 and 93 kD. The total patterns were used as the basis of numerical analysis. Most strains were clustered in four phenons at 91% similarity with the exception of six ungrouped strains. Overall similarity was high with all strains linked in the phenogram at ≥81%. Variation among strains was attributable principally to qualitative and quantitative band differences in the 47 to 56 kD (hypervariable) region of the C. pylori protein profile. From the analysis, ten different electropherotypes (EP-types) were identified. We demonstrated that differences were detectable among isolates from widely separated geographical locations as well as from the same location, although multiple isolates from two Peruvian patients had the same electropherotype. Our results indicate that determination of protein profiles provides the basis of a reproducible method for characterization of C. pylori isolates.
Similar content being viewed by others
References
Apel I, Kist M & Jacobs E (1987) Characterization of C. pylori by SDS-PAGE and detection of a specific antigen with human immune sera. In: Kaijser B & Falsen E (Eds) IVth International Workshop on Campylobacter Infections (pp 183–184) Gotterna, Kungälv
Axon ATR (1986) Campylobacter pyloridis: what role in gastritis and peptic ulcer? Br. Med. J. 293: 772
Costas M & Owen RJ (1987) Numerical analysis of electrophoretic protein patterns of Streptobacillus moniliformis strains from human, murine and avian infections. J. Med. Microbiol. 23: 303–311
Costas M, Holmes B & Sloss LL (1987a) Numerical analysis of electrophoretic protein patterns of Providencia rustigianii strains from human diarrhoea and other sources. J. Appl. Bacteriol. 63: 319–328
Costas M, Leach RH & Mitchelmore DL (1987b) Numerical analysis of PAGE protein patterns and the taxonomic relationships within the ‘Mycoplasma mycoides cluster’. J. Gen. Microbiol. 133: 3319–3329
Dooley CP & Cohen H (1988) The clinical significance of Campylobacter pylori. Annl. Int. Med. 108: 70–79
Fox JG, Edrise BM, Cabot EB, Beaucage C, Murphy JC & Prostak KS (1986) Campylobacter-like organisms isolated from gastric mucosa of ferrets. Am. J. Vet. Res. 47: 236–239
Hirschl AM (1987) Frequency of occurrence of Campylobacter pylori and analysis of the systemic and local immune response. Zbl. Bakt. Hyg. A 266: 526–542
Holmes B, Costas M & Sloss LL (1988) Numerical analysis of electrophoretic protein patterns of Providencia alcalifaciens strains from human faeces and veterinary specimens. J. Appl. Bacteriol. 64: 27–35
Itoh T, Yanagawa Y, Shingaki M, Takohashi M, Kai A, Ohashi M & Hamana G (1987) Isolation of Campylobacter pyloridis from human gastric mucosa and characterization of the isolates. Microbiol. Immunol. 31: 603–614
Jackman PJH, Feltham RKA & Sneath PHA (1983) A program in BASIC for numerical taxonomy of micro-organisms based on electrophoretic protein patterns. Microbios. Lett. 23: 87–98
Jones D (1986) Campylobacter pyloridis serology. Serodiagnosis Immunother. 112: 87–89
Jones D & Eldridge J (1987) Gastric Campylobacter-like organisms (GCLO) from man (“C. pyloridis”) compared with GCLO strains from the pig, baboon and ferret. In: Kaijser B & Falsen E (Eds) IVth International Workshop on Campylobacter Infections (p 44) Gotterna, Kungälv
Langenberg W, Rauws EAJ, Widjojokusomo A, Tytgat GNJ & Zanen HC (1986) Identification of Campylobacter pyloridis isolates by restriction endonuclease DNA analysis. J. Clin. Microbiol. 24: 414–417
Lau PP, DeBrunner-Vossbrinck B, Dunn B, Miotto K, MacDonell MT, Rollins DM, Pilledge CJ, Hespell RB, Colwell RR, Segin ML & Fox GE (1987) Phylogenetic diversity and position of the genus Campylobacter. System. Appl. Microbiol. 9: 231–238
Leaper S & Owen RJ (1981) Identification of catalase producing Campylobacter species based on biochemical characteristics and on cellular fatty acid composition. Curr. Microbiol. 31: 31–35
Marshall BJ (1986) Campylobacter pyloridis and gastritis. J. Infect. Dis. 153: 650–657
Marshall BJ & Goodwin CS (1987) Revised nomenclature of Campylobacter pyloridis. Int. J. Syst. Bacteriol. 37: 68
Marshall BJ, Royce H, Annear DI, Goodwin CS, Pearman JW, Warren JW & Armstrong JA (1984) Original isolation of Campylobacter pyloridis from human gastric mucosa. Microbios. Lett. 25: 83–88
Marshall BJ, Armstrong JA, McGechnie DB & Glancy RJ (1985) Attempts to fulfill Koch's postulates for pyloric campylobacter. Med. J. Aus. 142: 436–439
McNulty CAM (1986) Campylobacter pyloridis-associated gastritis. J. Infect. 13: 107–113
McNulty CAM & Dent JC (1987) Rapid identification of Campylobacter pylori (C. pyloridis) by preformed enzymes. J. Clin. Microbiol. 25: 1683–1686
Megraud F, Bonnet F, Garnier M & Lamouliatte H (1985) Characterization of “Campylobacter pyloridis” by culture, enzymatic prolife, and protein content. J. Clin. Microbiol. 22: 1007–1010
Morgan DR, Fitzpatrick PM, David KL & Kraft WG (1987) Susceptibility patterns of Campylobacter pyloridis. FEMS Microbiol. Lett. 42: 245–248
Newell DG (1987) Identification of the outer membrane proteins of Campylobacter pyloridis and antigenic cross-reactivity between C. pyloridis and C. jejuni. J. Clin. Microbiol. 133: 163–170
Owen RJ, Martin SR & Borman P (1985) Rapid urea hydrolysis by gastric campylobacters. Lancet i: 111
Paster BJ & Dewhirst FE (1988) Phylogeny of Campylobacters, Wolinellas, Bacteroides gracilis, and Bacteroides ureolyticus by 16S ribosomal ribonucleic acid sequencing. Int. J. Syst. Bacteriol. 38: 56–62
Pearson AD, Bamforth J, Booth L, Holdstock G, Ireland A, Walker C, Hawtin P & Millward-Sadler H (1984) Polyacrylamide gel electrophoresis of spiral bacteria from the gastric antrum. Lancet i: 1349–1350
Rathbone BJ, West AP, Wyatt JI, Johnson AW, Tompkins DS & Heatley RV (1986) Campylobacter pyloridis, urease, and gastric ulcers. Lancet ii: 400
Romaniuk PJ, Zoltowska B, Trust TJ, Lane DJ, Olsen GJ, Pace NR & Stahl DA (1987) Campylobacter pyloridi, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp.. J. Bacteriol. 169: 2137–2141
Taylor AJ, Costas M & Owen RJ (1987) Numerical analysis of electrophoretic protein patterns of Bacteroides ureolyticus clinical isolates. J. Clin. Microbiol. 25: 660–666
Tija TN, Hooper WES, Goodwin CS & Grubb WB (1987) Plasmids in Campylobacter pyloridi. Microbios Lett. 36: 7–11
VonWulffen H (1987) Low degree of relatedness between Campylobacter pyloridis and enteropathogenic Campylobacter species as revealed by DNA-DNA blot hybridization and immunoblot studies. FEMS Microbiol. Lett. 42: 129–133
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Owen, R.J., Costas, M., Morgan, D.D. et al. Strain variation in Campylobacter pylori detected by numerical analysis of one-dimensional electrophoretic protein patterns. Antonie van Leeuwenhoek 55, 253–267 (1989). https://doi.org/10.1007/BF00393854
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00393854