Skip to main content
SpringerLink
Log in

Novel glycopeptides, containing desmosine and isodesmosine, isolated from porcine aorta

  • Published:
Glycoconjugate Journal Aims and scope Submit manuscript

Abstract

Intima-media of porcine thoracic aorta were digested with pronase, after extraction of the saline-soluble matters and fat. A glycopeptide fraction was precipitated with 90% (vol/vol) ethanol from the 80% ethanol-soluble fraction of the trichloroacetic acid (7%)-soluble fraction of the pronase digest. The glycopeptide fraction was fractionated by affinity chromatography on concanavalin A (Con A)-Sepharose 4B, yielding 4 fractions (FA, FB, FC and FD). The most carbohydrate-rich fraction (FB) was further purified to a homogeneous state. The purified FB (FB-0.1) and all other fractions contained desmosine and isodesmosine. The major sugars in the fractions without or with low affinity for Con A (FA, FB, and FB-0.1) were glucosamine, galactose, mannose and sialic acid, while those in the fractions with high affinity for this lectin (FC and FD) were glucosamine, glucose and mannose. All the fractions contained glycine, aspartic acid (and/or asparagine), serine, proline, threonine, glutamic acid (and/or glutamine) and alanine as the major amino acids, amounting to approximately 80% of the total.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Robert L, Moczar M (1982) in Methods in Enzymology, Vol 82, eds. Cunningham LW, Frederiksen DW, Academic Press, New York, p 839–52.

    Google Scholar 

  2. Saito H, Yosizawa Z (1975) J Biochem 77:919–30.

    Google Scholar 

  3. Saito H, Ototani N, Yosizawa Z (1975) J Biochem 77:931–38.

    Google Scholar 

  4. Gibson MA, Grant ME, Jackson DS (1982) Connect Tissue Res 10:145–60.

    Google Scholar 

  5. Moczar M, Phan-Dinh-Tuy B, Moczar E, Robert L (1983) Biochem J 211:257–65.

    Google Scholar 

  6. Bressan GM, Castellani I, Colombatti A, Volpin D (1983) J Biol Chem 258:13262–67.

    Google Scholar 

  7. Aikawa J, Munakata H, Isemura M, Ototani N, Yosizawa Z (1984) Tohoku J Exp Med, in press.

  8. Munakata H, Yosizawa Z (1980) J Biochem 87:1559–65.

    Google Scholar 

  9. Robert B, Szigeti M, Derouette J-C, Robert L, Bouissou H, Fabre M-T (1971) Eur J Biochem 21:507–16.

    Google Scholar 

  10. John R, Thomas J (1972) Biochem J 127:261–69.

    Google Scholar 

  11. Paz MA, Keith DA, Gallop PM (1982) in Methods in Enzymology Vol 82, eds. Cunningham LW, Frederiksen DW, Academic Press, New York, p 517–87.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biochemistry, Tohoku University School of Medicine, 2-1, Seiryo-machi, 980, Sendai, Japan

    J Aikawa, H Munakata, M Isemura & Z Yosizawa

Authors
  1. J Aikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H Munakata
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. M Isemura
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Z Yosizawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Aikawa, J., Munakata, H., Isemura, M. et al. Novel glycopeptides, containing desmosine and isodesmosine, isolated from porcine aorta. Glycoconjugate J 1, 9–15 (1984). https://doi.org/10.1007/BF01875408

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01875408

Key words

Search

Navigation