Abstract
The injection of 3-dehydroecdysone (3dhE, 5 μ/g), the major ecdysteroid secreted by the Y-organ of crayfishProcambarus clarkii, resulted in apolysis within about 5 days. The hormonal response at the molecular level was investigated by injection of the radio-labeled compound; within 3 h of injection of [3H]3dhE, most radio-isotope was found in the extracted epidermal tissues and identified as ecdysone, 20-hydroxyecdysone (20E), and their 3α-hydroxy epimers. The biotransformation was undoubtedly performed in the peripheral area of the Y-organ. Cleavage of the polar conjugates, using an enzyme fromHelix pomatia, gave all of the above ecdysteroids including 3dhE. It was also found that the biosynthetic site of 3dhE was different from that of ecdysone at the subcellular level of the Y-organ.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Chang, E. S., and O'Connor, J. D., Proc. natl Acad. Sci. USA74 (1977) 615.
Lachaise, F., Meister, M. F., Hétru, C., and Lafont, R., Molec. cell. Endocr.45 (1986) 253.
Lachaise, F., Carpentier, G., Sommé, G., Colardeau, J., and Beydon, P., J. exp. Zool.252 (1989) 283.
Spaziani, E., Rees, H. H., Wang, W. L., and Watson, R. D., Molec. cell. Endocr.66 (1987) 17.
Karlson, P., and Koolman, J., Insect Biochem.3 (1973) 409.
Sakurai, S., Warren, J. T., and Gilbert, L. I., Arch. Insect Biochem. Physiol.10 (1989) 179.
Kiriishi, S., Rountree, D. B., Sakurai, S., and Gilbert, L. I., Experientia46 (1990) 716.
Sonobe, H., Kamba, M., Ohta, K., Ikeda, M., and Naya, Y., Experientia47 (1991) 948.
Naya, Y., Kishida, K., Sugiyama, M., Murata, M., Miki, W., Ohnishi, M., and Nakanishi, K., Experientia44 (1988) 50.
Dinan, L., and Rees, H. H., Steroids32 (1978) 629.
Krishnakumaran, A., and Schneiderman, H. A., Biol. Bull.139 (1970) 520.
Hétru, C., Luu, B., and Hoffmann, J. A., in: Methods in Enzymology, vol. 111, p. 417. Eds J. H. Law and H. C. Rilling, Academic Press, Orlando, Florida 1985.
Skinner, D. M., in: The Biology of Crustacea, vol. 9, p. 94. Eds D. E. Bliss and L. H. Mantel. Academic Press, Orlando, Florida 1985.
Richards, G., J. Insect Physiol.24 (1978) 329.
Skinner, D. M., in: The Biology of Crustacea, vol. 9, p. 81. Eds D. E. Bliss and L. H. Mantel Academic Press. Orlando, Florida 1985.
Snyder, M. J., and Chang, E. S., Gen. comp. Endocr.81 (1991) 133.
Snyder, M. J., and Chang, E. S., Biol. Bull.180 (1991) 475.
Lachaise, F., Le Roux, A., Hubert, M., and Lafont, R. J., Crust. Biol.13(2) (1993) 198.
Naya, Y., Ohnishi, M., Ikeda, M., Miki, W., and Nakanishi, K., Proc. natl Acad. Sci. USA86 (1989) 6826.
Ohnishi, M., Nakanishi, K., and Naya, Y., Chimicaoggi9 (1991) 53.
Warren, J. T., Sakurai, S., Rountree, D. B., and Gilbert, L. I., J. Insect Physiol.34 (1988) 571.
Christ, B., and Sedlmeier, D., Int. J. Biochem.19 (1987) 79.
Daig, C., and Spindler, K.-D., Molec. cell. Endocr.32 (1983) 73.
Clever, U., Clever, I., Storbeck, J., and Young, N. L., Devl Biol.31 (1973) 47.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ikeda, M., Naya, Y. The biotransformation of tritiated 3-dehydroecdysone by crayfish,Procambarus clarkii . Experientia 49, 1101–1105 (1993). https://doi.org/10.1007/BF01929922
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01929922