Abstract
It was demonstrated that excised Y-organs of the crayfish,Procambarus clarkii, synthesize in vitro 3-dehydroecdysone (3-DHE) as the major product, together with small amounts of ecdysone. Both were identified by immunological and spectroscopic methods. The increase of ecdysteroidogenesis in the Y-organs was accompanied by an increase of the major free ecdysteroid, 20-hydroxyecdysone, in the hemolymph. This suggests a physiological role of 3-DHE, the details of which are still to be elucidated.
Similar content being viewed by others
References
Chang, E. S., and O'Connor, J. D., Proc. natl Acad. Sci. USA74 (1977) 615.
Keller, R., and Schmid, E., J. comp. Physiol. B130 (1979) 347.
Lachaise, F., Meister, M. F., Hetru, C., and Lafont, R., Molec. cell. Endocr.45 (1986) 253.
Spaziani, E., Rees, H. H., Wang, W. L., and Watson, R. D., Molec. cell. Endocr.66 (1989) 17.
Naya, Y., and Sonobe, H., Heredity44 (1990) 62.
Warren, J. T., Sakurai, S., Rountree, D., Gilbert, L. I., Lee, S., and Nakanishi, K., Proc. natl Acad. Sci. USA85 (1988) 985.
Kiriishi, S., Rountree, D. B., Sakurai, S., and Gilbert, L., Experientia46 (1990) 716.
Carrow, G. W., Calabrese, R. L., and Williams, C. M., Proc. natl Acad. Sci. USA78 (1981) 5866.
Dinan, L., and Rees, H. H., Steroids32 (1978) 2368.
Webster, S. G., and Keller, R., in: Ecdysone; From Chemistry to Mode of Action, p. 211. Ed. J. Koolman. George Thieme Verlag, Stuttgart, New York 1989.
Naya, Y., Ohnishi, M., Ikeda, M., Miki, W., and Nakanishi, K., Proc. natl Acad. Sci. USA86 (1989) 6826.
Warren, J. T., and Gilbert, L. I., in: Immunologic Techniques in Insect Biology, p. 181. Eds L. I. Gilbert and T. A. Miller. Springer-Verlag, Heidelberg 1988.
Charmantier-Daures, M., and Vernet, G., C.r. Acad. Sci. Paris278 (1974) 3367.
Connat, J. L., and Diehl, P. A., Insect Biochem.16 (1986) 91.
Snyder, J. M., and Chang, E. S., Gen. comp. Endocr.81 (1991) 133.
Sakurai, S., Warren, J. T., and Gilbert, L. I., Archs Insect Biochem. Physiol.10 (1989) 179.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sonobe, H., Kamba, M., Ohta, K. et al. In vitro secretion of ecdysteroids by Y-organs of the crayfish,Procambarus clarkii. Experientia 47, 948–952 (1991). https://doi.org/10.1007/BF01929889
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/BF01929889