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Purification and characterization of aβ-glucosidase (linamarase) from the haemolymph ofZygaena trifolii Esper, 1783 (Insecta, Lepidoptera)

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Summary

A β-glucosidase (linamarase) was purified 52-fold with a recovery of 27% from the haemolymph of the larvae ofZygaena trifolii, ESPER, 1783 (Lepidoptera, Zygaenidae). The final enzyme preparation was found to be nearly homogeneous on both disc polyacrylamide gel electrophoresis and SDS-polyacrylamide gel electrophoresis. The molecular weight of the enzyme was determined to be about 130 kDa; it consisted of two subunits of about 66 kDa. The enzyme showed an optimum between pH 4.5 and 5 with linamarin and a broad optimum between pH 3.5 and 6.5 for p-nitrophenyl-β-D-glucoside; the temperature optimum was 40°C. The β-glucosidase showed a high specificity for its endogenous substrates linamarin and lotaustralin. Among the other natural and artificial substrates tested, only prunasin and p-nitrophenyl-β-D-glucoside were hydrolyzed by the enzyme, whereas linustatin, salicin, cellobiose and trehalose were not. The enzyme is strongly inhibited by β-glucosylpiperidine.

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Authors and Affiliations

  1. Institut für Pharmazeutische Biologie und Phytochemie der Westfälischen Wilhelms-Universität, Hittorfstr. 56, D-4400, Münster, (Federal Republic of Germany)

    S. Franzl, I. Ackermann & A. Nahrstedt

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  1. S. Franzl
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  2. I. Ackermann
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  3. A. Nahrstedt
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Franzl, S., Ackermann, I. & Nahrstedt, A. Purification and characterization of aβ-glucosidase (linamarase) from the haemolymph ofZygaena trifolii Esper, 1783 (Insecta, Lepidoptera). Experientia 45, 712–718 (1989). https://doi.org/10.1007/BF01974565

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  • DOI: https://doi.org/10.1007/BF01974565

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