Summary
Replicative DNA synthesis in permeable mouse sarcoma cells was more sensitive to ethidium bromide (EtBr) than bleomycin-induced unscheduled DNA synthesis (UDS). A similar difference in sensitivity to EtBr was observed between DNA polymerases α and β. The difference in sensitivity to EtBr of replicative DNA synthesis and UDS in the present system seems to reflect mainly the sensitivity difference between DNA polymerases α and β.
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Acknowledgments. The authors wish to thank Nippon Kayaku Co. (Tokyo, Japan) for providing copper-free bleomycin A2. This research was supported in part by a grant from the Japan Ministry of Education, Science and Culture.
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Seki, S., Oda, T. Differential sensitivity to ethidium bromide of replicative DNA synthesis and bleomycin-induced unscheduled DNA synthesis in permeable mouse sarcoma cells1. Experientia 40, 869–871 (1984). https://doi.org/10.1007/BF01952001
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DOI: https://doi.org/10.1007/BF01952001