Summary
The nuclear organizer (NOR) was studied in nucleoli of dormant meristems cells ofAllium cepa L. bulbs by means of electron and light microscopy. Nucleoli are mainly fibrillar and they present a clear segregation between fibrillar and granular components. In these cells, the NOR, whose electron density is lower than the NOR heterochromatin, appears as a compact single mass located inside the nucleolus. These ultrastructural characteristics make NOR visualization possible at the light microscopic level by the complementary use of both silver impregnation and Feulgen staining techniques.
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Acknowledgments. We gratefully acknowledge Drs C. De La Torre, P. Esponda, B. Schwartzman and R. López for the suggestions and critical reading of this manuscript. The work has been partially supported by the Acuerdo de Cooperación Científica y Tecnológica entre C.S.I.C. (España) y Universidad de Chile (Proyecto No. 1), by Proyecto B 1245-811-2 SDCACI, Universidad de Chile and by Programa Multiinstitucional O.E.A. ‘Biología de la Reproducción’.
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Sans, J. The nucleolar organizer in meristem cells ofAllium cepa L. bulbs. Experientia 38, 796–797 (1982). https://doi.org/10.1007/BF01972276
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DOI: https://doi.org/10.1007/BF01972276