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A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases

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Summary

Heterologous antiserum to the 3 biotin-dependent carboxylases was prepared by selective removal of these enzymes from human liver on an avidin-sepharose column. A carboxylase-avidin-sepharose matrix was used as an antigen to produce anti-carboxylase antibodies. The resultant antisera can be used to purifiy the specific carboxylases, to prepare monoclonal antibodies to these enzymes or to study inherited carboxylase deficiencies and biotin-dependent intermediary metabolism.

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Author notes

  1. Catherine McKeon

    Present address: Gene Regulation Section, National Cancer Institute, National Institutes of Health, 20205, Bethesda, Maryland, USA

Authors and Affiliations

  1. Departments of Human Genetics and Pediatrics, Medical College of Virginia, 23298, Richmond, Virginia, USA

    Catherine McKeon & B. Wolf

Authors
  1. Catherine McKeon
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  2. B. Wolf
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Additional information

The authors thank Dr Emmanuel Shapira for his helpful suggestions and Patricia Tuck for her technical assistance. This work was supported by NIH research grants AM25675 and AM26127. Barry Wolf is a recipient of an NIH Research Career Development Award (AM00677) and is aided by a Basil O'Connor Starter Research Grant from the National Foundation—March of Dimes (5–263). This is paper No. 137 from the Department of Human Genetics — Medical College of Virginia.

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McKeon, C., Wolf, B. A simple method for the preparation of antibodies to the mitochondrial biotin-dependent carboxylases. Experientia 38, 281–282 (1982). https://doi.org/10.1007/BF01945114

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