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Detection of human ADCC activity using automated flow cytometry

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Summary

Using automated flow cytometry techniques we have developed a rapid assay to measure human antibody-dependent cell-mediated cytotoxicity (ADCC). By staining cell cultures for DNA content, chick red blood cell targets can be readily distinguished from human effector cells and ADCC can be measured by changes in their relative proportions. The sensitivity and rapidity of the assay is shown by the finding that at an effector to target ratio of only 2:1, 42% killing can be detected after 1 h incubation.

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References

  1. Acknowledgment. The opinions and assertions contained herein are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. The experiments reported herein were conducted according to the principles set forth in the current edition of the ‘Guide for the Care and Use of Laboratory Animals’, Institute of Laboratory Animal Resources, National Research Council. We thank Dr J. Petricciani for continuous support and Ms E. Kirshbaum for typing the manuscript.

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Authors and Affiliations

  1. Division of Pathology, Bureau of Biologics, 8800 Rockville Pike, 20205, Bethesda, MD

    A. M. Attallah, P. D. Noguchi, T. Folks & C. T. Noguchi

  2. Naval Medical Research Institute, 20014, Bethesda, MD

    A. M. Attallah, P. D. Noguchi, T. Folks & C. T. Noguchi

  3. Laboratory of Chemical Biology, National Institute of Arthritis, Metabolic and Digestive Diseases, 8800 Rockville Pike, 20205, Bethesda, MD, USA

    A. M. Attallah, P. D. Noguchi, T. Folks & C. T. Noguchi

Authors
  1. A. M. Attallah
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  2. P. D. Noguchi
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  3. T. Folks
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  4. C. T. Noguchi
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Attallah, A.M., Noguchi, P.D., Folks, T. et al. Detection of human ADCC activity using automated flow cytometry. Experientia 36, 124–125 (1980). https://doi.org/10.1007/BF02004013

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  • DOI: https://doi.org/10.1007/BF02004013

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