Skip to main content
SpringerLink
Log in

The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism

  • Specialia
  • Published:
Experientia Aims and scope Submit manuscript

Summary

The influence of a) tissue culture medium (RPMI), b) homologous plasma (HP), and c) fetal calf serum (FCS) on lymphocytic cortisol metabolism was compared to that of phosphate buffered saline alone. RPMI was found to enhance the conversion rate 1.71 times, whereas HP and FCS enhanced it about 3.2 times. Raising the temperature of the HP and FCS to 100°C before incubation reduced the enhancing effect to the level of that obtained with RPMI.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. The Clinical Laboratory, Beilinson Medical Center.

  2. T.F. Dougherty, D.L. Berliner and M.L. Berliner, Metabolism10, 966 (1961).

    Google Scholar 

  3. D.L. Berliner and T.F. Dougherty, Pharmac. Rev.12, 329 (1961).

    Google Scholar 

  4. J.S. Jenkins and N.H. Kemp, J. clin. Endocr.29, 1217 (1969).

    PubMed  Google Scholar 

  5. A. Klein, H. Kaufmann, S. Mannheimer and H. Joshua, Israel J. med. Sci.14, 404 (1978).

    Google Scholar 

  6. A. Klein, H. Kaufmann, S. Mannheimer and H. Joshua, Metabolism27, 731 (1978).

    Article  PubMed  Google Scholar 

  7. A.H. Dodge, A.K. Christensen and R.B. Clayton, Endocrinology87, 254 (1970).

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. The Endocrinological Laboratory and the Clinical Laboratory, Beilinson Medical Center, Petach-Tiqva, (Israel)

    A. Klein, H. Kaufmann, R. Arie, H. G. D. Hoogervorst-Spalter & H. Joshua

Authors
  1. A. Klein
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. H. Kaufmann
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. Arie
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. H. G. D. Hoogervorst-Spalter
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. H. Joshua
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

This work was supported by The Israel Cancer Research Fund N.Y. (George and Rose Blumenthal Research Fellowship for Hodgkins Disease). The authors are grateful to Dr Malchi and his staff from the Beilinson Hospital Blood Bank, for supplying us with buffy coats and to Mr Z. Klein for drawing the figure.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Klein, A., Kaufmann, H., Arie, R. et al. The influence of homologous plasma and fetal calf serum on human lymphocytic cortisol metabolism. Experientia 35, 114–115 (1979). https://doi.org/10.1007/BF01917912

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01917912

Keywords

Search

Navigation