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A cell culture substrate obtained from heat-fused collagen

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Summary

Coverslips coated with rat-tail collagen dried at 37°C were placed in a hot-air sterilizing oven at 160°C for 2 h. The resulting transparent sterile film was found to be a useful multipurpose substrate for cell culture and for subsequent histological sectioning.

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Authors and Affiliations

  1. School of Biological Sciences, The University of Sydney, Zoology Building A08, 2006, New South Wales, Sydney, Australia

    E. M. Stephenson

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  1. E. M. Stephenson
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Additional information

The initial observation leading to the use of fused collagen was made by the author while working at the Strangeways Research Laboratory, Cambridge. A stock of MM96 cells was kindly supplied by Dr.J. H. Pope, Queensland Institute of Medical Research.

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Stephenson, E.M. A cell culture substrate obtained from heat-fused collagen. Experientia 31, 1473–1474 (1975). https://doi.org/10.1007/BF01923250

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  • DOI: https://doi.org/10.1007/BF01923250

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