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A simple device for semiautomatic washing of adhering cells in Microtest plates

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Zusammenfassung

Es wird ein einfacher Apparat beschrieben, der schnelles und gleichmässiges Waschen von adhärenten Zellen in Microtest II 3040 Platten (Falcon) erlaubt. Eine Gruppe von 12 zylindrischen Vertiefungen mit flachem Boden wird gleichzeitig behandelt. Der Apparat ist einerseits an Sog und anderseits an eine automatische Spritze mit verstellbarem Volumen (Cornwall) angeschlossen. Er fand ausgiebig Verwendung in einem in vitro Testsystem für direkte zellvermittelte Zytotoxizität. Als Zielzellen dienten51Cr markierte peritoneale Macrophagen.

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References

  1. F. Harder, M. Demarchi, G. Trinchieri, G. Thiel, Transplantation,17, 551 (1974).

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Author notes

  1. F. Harder

    Present address: Research Division, Department of Surgery of the University of Basel, Kantonsspital, CH-4004, Basel, Switzerland

  2. G. Thiel

    Present address: Division of Transplantation, Department of Medicine of the University of Basel, Kantonsspital, CH-4004, Basel, Switzerland

Authors and Affiliations

  1. Research Division, Department of Surgery of the University of Basel, Kantonsspital, CH-4004, Basel, Switzerland

    G. Thiel

  2. Division of Transplantation, Department of Medicine of the University of Basel, Kantonsspital, CH-4004, Basel, Switzerland

    F. Harder

Authors
  1. F. Harder
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  2. G. Thiel
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Harder, F., Thiel, G. A simple device for semiautomatic washing of adhering cells in Microtest plates. Experientia 30, 839–840 (1974). https://doi.org/10.1007/BF01924222

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  • DOI: https://doi.org/10.1007/BF01924222

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