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Genetic evidence for the tetramer structure of glyceraldehyde-3-phosphate dehydrogenase

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Zusammenfassung

Es wurden beiXiphophorus maculata undXiphophorus helleri zwei Isozyme von Glyceraldehyd-3-phosphat-Dehydrogenase nachgewiesen. Die biochemisch-genetischen U Untersuchungen deuten auf eine Tetrameren-Struktur.

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Literatur

  1. W. C. Watson andL. J. Banaszak, Nature, Lond.204, 918 (1964).

    Article  CAS  PubMed  Google Scholar 

  2. W. F. Harrington andG. M. Karr, J. molec. Biol.13, 855 (1965).

    Article  Google Scholar 

  3. J. I. Harris andR. N. Perham, J. molec. Biol.13, 876 (1965).

    Article  CAS  Google Scholar 

  4. C. L. Markert, Science140, 1329 (1963) andC. R. Shaw, Brookhaven Symp. Biol.17, 117 (1964).

    Article  CAS  PubMed  Google Scholar 

  5. Tissue from eye, muscle, liver and brain was frozen on dry ice, homogenized in glass homogenizers in cold 1/20 dilution of Amphibian Ringer's solution that was buffered with 0.001M Tris-HCl at pH 7.5. The ratio of tissue to homogenizing medium was 1∶3 weight/volume.

  6. Both strains are the descendents of fish obtained from Klaus Kallman of the New York Zoological Society.

  7. The method of vertical starch gel electrophoresis and G3PD staining was essentially that ofC. R. Shaw andR. Prasad, Biochem. Genet.4, 297 (1970). Their buffer system I (Tris-citrate, pH 7.0) was used with the modification of adding 0.06 ml β-mercaptoethanol and 40 mg nicotinamide adenine dinucleotide for 600 ml of starch gel. These 2 ingredients proved essential for demonstration of G3PD activity in starch gels.

    Article  CAS  PubMed  Google Scholar 

  8. Hybrids were obtained according to the artificial insemination method ofE. Clark, Science112, 722 (1950).

    Article  CAS  PubMed  Google Scholar 

  9. H. G. Lebherz andW. J. Rutter, Science157, 1198 (1967).

    Article  CAS  PubMed  Google Scholar 

  10. This research was supported in part by research grant No. GM 15597 and training grant No. CA 5047, both from NIH.

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Authors and Affiliations

  1. The University of Texas Graduate School of Biomedical Sciences, and M. D. Anderson Hospital and Tumor Institute, 77025, Houston, Texas, USA

    D. A. Wright, M. J. Siciliano & J. N. Baptist

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  1. D. A. Wright
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  2. M. J. Siciliano
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  3. J. N. Baptist
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Wright, D.A., Siciliano, M.J. & Baptist, J.N. Genetic evidence for the tetramer structure of glyceraldehyde-3-phosphate dehydrogenase. Experientia 28, 888–889 (1972). https://doi.org/10.1007/BF01924924

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