Summary
With growing cells of a Nocardia sp., isolated from soil, the degradation of 5-pregnene-3β, 20β-diol into 3-[5-oxo-7aβ-methyl-1β (1′β-hydroxo)-ethyl-3aα-perhydroindane-4α]-propionic acid was investigated. The results show that iron is essential for production of the perhydroindanpropionic acid, that this production is greatly enhanced by the presence of calcium and that it is maximal in the pH range 7.0–7.5.
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Abbreviations
- PD:
-
5-pregnene-3β, 20β-diol (pregnendiol)
- PDSA:
-
3-[5-oxo-7aβ-methyl-1β(1′β-hydroxo)-ethyl-3aα-perhydroindane-4α]-propionic acid (pregnendiol-secoacid)
- PSA:
-
3-[5-oxo-7aβ-methyl-1β-acetyl-3aα-perhydroindane-4α]-propionic acid (progesterone-secoacid)
- EDTA:
-
Ethylendiamintetracetic acid
- DMSO:
-
Dimethylsulfoxide
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Tan, TL., Strijewski, A. & Wagner, F. Degradation of 5-pregnene-3β, 20β-diol by a Nocardia sp.. Archiv. Mikrobiol. 87, 249–256 (1972). https://doi.org/10.1007/BF00424885
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DOI: https://doi.org/10.1007/BF00424885