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Isolation of an antigenic determinant from flagellar glycoproteins of Chlamydomonas eugametos

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Abstract

A labeled oligosaccharide fraction, obtained by reductive β-elimination of an extract of minus mating-type flagella of Chlamydomonas eugametos, is shown to contain a disaccharide fraction which is bound by an antiserum raised against the sexual agglutination factor present at the flagellar surface of that species. The fraction contains arabinose and galactose. It is argued that galactose and/or arabinose may be a functional constituent of the agglutination factor.

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Abbreviations

mt +/- :

mating type plus or minus

PAS:

periodic acid Schiff

GTC:

guanidine thiocyanate

IgG:

immunoglobulin G

PBS:

phosphate-buffered saline

SDS:

sodium dodecyl sulfate

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Authors and Affiliations

  1. Department of Plant Physiology, University of Amsterdam, Kruislaan 318, NL-1098 SM, Amsterdam, The Netherlands

    Peter F. Lens, Frans Olofsen, Piet van Egmond, Alan Musgrave & Herman van den Ende

Authors
  1. Peter F. Lens
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  2. Frans Olofsen
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  3. Piet van Egmond
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  4. Alan Musgrave
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  5. Herman van den Ende
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Additional information

The investigations were supported by the Foundation for Fundamental Biological Research (BION), which is subsidized by the Netherlands Organization for the Advancement of Pure Research (ZWO).

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Lens, P.F., Olofsen, F., van Egmond, P. et al. Isolation of an antigenic determinant from flagellar glycoproteins of Chlamydomonas eugametos . Arch. Microbiol. 135, 311–314 (1983). https://doi.org/10.1007/BF00413487

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  • DOI: https://doi.org/10.1007/BF00413487

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