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Elektronenmikroskopische Darstellung peroxydatischer Aktivitäten bei Phaseolus vulgaris nach Infektion mit Uromyces phaseoli typica

Electronmicroscopic demonstration of peroxidase activities in Phaseolus vulgaris after infection with Uromyces phaseoli typica

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Summary

Peroxidase activity in Phaseolus vulgaris (cultivar Favorit), infected with Uromyces phaseoli typica, was studied with the help of an electronmicroscope. The plant material was prefixed three days after inoculation and 3,3-diaminobenzidine was used as a substrate to detect the enzyme activity.

The mitochondrial membranes showed an enhanced enzyme activity (due to a cytochrome oxidase or possibly due to a cytochrome peroxidase) in the infected cell. There was no change in the structure and catalase activity of peroxisomes of the host. No catalase activity was detected in the fungus.

A layer with evident peroxidase activity is seen outside the cell wall. This layer is sometimes thickened especially when it is in touch with intercellular hyphae.

The penetration site of the haustorium has been intensively studied. Activity was observed in the neck region (from the penetration site of the haustorium to the neckband) in the zone between the host plasmalemma and the plasmalemma of the fungus. Some activity was also seen on the cisternae of the endoplasmic reticulum surrounding the neck. In a few preparations, activity was also found between sheath and wall of the haustorium.

Zusammenfassung

Bei der Wirt-Parasit-Kombination Phaseolus vulgaris (cv. Favorit) und Uromyces phaseoli typica wird die Aktivität peroxydatischer Enzyme am dritten Tag nach Infektion elektronenmikroskopisch dargestellt. Die Aktivität in den Mitochondrien (möglicherweise eine Cytochromoxydase oder Cytochromperoxydase) ist in der infizierten Zelle erhöht. Die Peroxisomen (sie enthalten eine Katalase) bleiben in der infizierten Zelle unverändert. Im Pilz wird keine Katalaseaktivität gefunden.

Die Schicht mit peroxydatischer Aktivität auf der Außenseite der Zellwand (Zellwandperoxydase) ist an der Berührungsstelle Hyphe—Wirtswand oft verdickt. An der Eintrittsstelle des Haustoriums in die Zelle wird Aktivität auf der Wand des Haustoriumhalses und auf den Cisternen des benachbarten ribosomenbesetzten endoplasmatischen Reticulums beobachtet. Manchmal findet man auch eine Schicht mit peroxydatischer Aktivität zwischen Scheide und Wand des Haustoriums.

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  1. Institut für Pflanzenpathologie und Pflanzenschutz der Universität Göttingen, Göttingen, Deutschland

    K. Mendgen & W. H. Fuchs

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  1. K. Mendgen
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  2. W. H. Fuchs
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Mendgen, K., Fuchs, W.H. Elektronenmikroskopische Darstellung peroxydatischer Aktivitäten bei Phaseolus vulgaris nach Infektion mit Uromyces phaseoli typica . Archiv. Mikrobiol. 88, 181–192 (1973). https://doi.org/10.1007/BF00421844

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