Summary
A yeast, Candida boidinii, isolated from soil, capable of growing on a medium containing methanol as the only carbon source is described. Biotin is required in very low concentration as a growth factor.
In a study on the effect of the methanol concentration on the cell growth under favorable conditions, the cell yield was 2.3 g (dry weight) with 1% (v/v) methanol and 8.3 g with 4% methanol per 1000 ml of culture medium. However, the growth was inhibited by 5% methanol. The strain assimilated carbohydrate and ethanol faster than methanol or lactate. The enzymes for the methanol metabolism are probably constitutive.
Optimal conditions for rapid growth and high cell yeild from methanol were found to be: 28°C, NH4 + as nitrogen source and pH 5.0.
The cell composition was as follows: 42.81% C, 7.23% H and 5.54% N. Amino acids in the cells were analyzed by the amino acid autoanalyzer.
Zusammenfassung
Aus einer Bodenprobe wurde eine Hefe, Candida boidinii, isoliert, welche auf Methanol als einziger C-Quelle wachsen kann. Als Wachstumsfaktor benötigt dieser Hefestamm Biotin in sehr geringer Konzentration.
Unter günstigen Kulturbedingungen beträgt die Zellausbeute pro 1000 ml Kulturmedium 2,3g Trockenmasse bei Zugabe von 1% (v/v) Methanol, und 8,3g bei 4% Methanol. Das Wachstum wird bei Zusatz von 5% Methanol zum Minimalmedium vollständig gehemmt. Der Stamm verwertet Kohlenhydrate und Äthanol schneller als Methanol oder Milchsäure. Die Enzyme für den Methanol-Stoffwechsel scheinen jedoch konstitutiv zu sein.
Die optimalen Kulturbedingungen für kurze Generationszeiten und hohe Zellausbeuten auf Methanol sind: 28°C, NH4 + als Stickstoffquelle und pH 5,0. Die Elementaranalyse ergab folgende Werte für die Zusammensetzung der Hefezellen: 42,81% C, 7,23% H und 5,54% N. Die Aminosäuren (in den Zellen) wurden mit dem Aminosäure-Analysator quantitativ bestimmt.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literatur
Anthony, C., Dunstan, P. M., Drabble, W. T.: The involvement of glycollate in the pathway for ethanol assimilation on Pseudomonas AM 1. Biochem. J. 124, 76p (1971).
Anthony, C., Zatman, L. J.: The microbial oxidation of methanol. I. Isolation and properties of Pseudomonas sp. M 27. Biochem. J. 92, 609–613 (1964).
——: The microbioal oxidation of methanol. The alcohol dehydrogenase of Pseudomonas sp. AM 27. Biochem. J. 96, 808–812 (1965).
Dworkin, M., Foster, J. W.: Studies on Pseudomonas methanica (Söhngen) nov. comb. J. Bact. 72, 646–659 (1956).
Harder, W., Quayle, J. R.: Aspects of glycine and serine biosynthesis during growth of Pseudomonas AM 1 on C1 compounds. Biochem. J. 121, 763–769 (1971).
Kaneda, T., roxburgh, J. M.: A methanol-utilizing bacterium. Canad. J. Microbiol. 5, 87–98 (1959).
Kemp, M. B., Quayle, J. R.: Microbial growth on C1 compounds. Uptake of (14C) formaldehyde and (14C) formate by methane-grown Pseudomonas methanica and determination of the hexose labelling pattern after brief incubation with (14C) methanol. Biochem. J. 102, 94–102 (1967).
Large, P. J., Peel, D., Quayle, J. R.: Microbial growth on C1 compounds. 3. Distribution of radioactivity in metabolites of methanol-grown Pseudomonas AM 1 after incubation with (14C) methanol and (14C) bicarbonate. Biochem. J. 82, 483–488 (1962).
Lawrence, A. J., Kemp, M. B., Quayle, J. R.: Synthesis of cell constituents by methane-grown Methylococcus capsulatus and Methanomonas methanooxidans. Biochem. J. 116, 631–639 (1970).
—, Quayle, J. R.: Alternative carbon assimilation path-pathways in methaneutilizing bacteria. J. gen. Microbiol. 63, 371–374 (1970).
Naguib, M., Overbeck, J.: On methane oxidizing bacteria in fresh waters. I. Introduction to the problem and investigations on the presence of obligate methane oxidizers. z. allg. Mikrobiol. 10, 17–36 (1970).
Ogata, K., Nishikawa, H., Ohzugi, M.: A yeast capable of utilizing methanol. Agric. biol. Chem. 33, 1519–1520 (1969).
———, Tochikura, T.: Studies on the production of yeast. II. The cultural conditions of methanol assimilating yeast, Kloeckera sp. No 2201. J. Ferment. Technol. 48, 470–477 (1970).
Okanishi, M., Gregory, K. F.: Isolation of mutants of Candida tropicalis with increased methionine content. Canad. J. Microbiol. 16, 1139–1143 (1970).
Peel, D., Quayle, J. R.: Microbial growth on C1 compounds. 1. Isolation and characterization of Pseudomonas AM 1. Biochem. J. 81, 465–469 (1961).
Ribbons, D. W., Harrison, J. E., Wadzinsky, A. M.: Metabolism of single carbon compounds. Ann. Rev. Microbiol. 24, 135–158 (1970).
Rose, A. H., Harrison, J. S.: The yeasts. London-New York: Academic Press 1969.
Vary, P. S., Johnson, M. J.: Cell yields of bacteria grown on methane. Appl. Microbiol. 15, 1473–1478 (1967).
Whittenbury, R.: Microbial utilization of methane. Proc. Biochem. 4, 51–56 (1969).
—, Phillips, K. C., Wilkinson, J. F.: Enrichment, isolation and some properties of methane-utilizing bacteria. J. gen. Microbiol. 61, 205–218 (1970).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Sahm, H., Wagner, F. Mikrobielle Verwertung von Methanol. Archiv. Mikrobiol. 84, 29–42 (1972). https://doi.org/10.1007/BF00408080
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF00408080