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Bacillus vini sp. nov. isolated from alcohol fermentation pit mud

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Abstract

A novel aerobic, Gram-stain-positive, sporogenous, rod-shaped bacterium, designated LAM0415T, was isolated from an alcohol fermentation pit mud sample collected from Sichuan Luzhou-flavour liquor enterprise in China. The isolate was found to be able to grow at NaCl concentrations of 0–10 % (w/v) (optimum: 1.0 %), 10–50 °C (optimum: 30–35 °C) and pH 3.0–10.0 (optimum: 7.0–8.0). Phylogenetic analysis of 16S rRNA gene sequences indicated that the new isolate belonged to the genus Bacillus and was closely related to Bacillus sporothermodurans DSM 10599T and Bacillus oleronius DSM 9356T, with 98.4 and 97.2 % sequence similarity, respectively. The DNA–DNA hybridization values between strain LAM0415T and the two reference strains were 33.3 ± 1.2 and 42.8 ± 0.8 %, respectively. The genomic DNA G+C content was 35.2 mol% as determined by the T m method. The major fatty acids were determined to be iso-C15:0, anteiso-C15:0 and anteiso-C17:0. The predominant menaquinones were identified as MK7 and MK8. The major polar lipids were found to be diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified phospholipid and four unidentified glycolipids. The diagnostic amino acid of the cell wall peptidoglycan was determined to be meso-diaminopimelic acid. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain LAM0415T (=ACCC 06413T = JCM 19841T) represents the type strain of a novel species of the genus Bacillus, for which the name Bacillus vini sp. nov. is proposed.

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Acknowledgments

This work was supported by the open funds of Key Laboratory of Microbial Resources Collection and Preservation (Ministry of Agriculture, MOA, 2015), the Special Fund for Agro-scientific Research in the Public Interest (No.201403019), the National Nonprofit Institute Research Grant of CAAS (No.2014-30) and National Key Technology R&D Program of China (No.2013BAD05B04F02).

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    Authors and Affiliations

    1. College of Environmental and Chemical Engineering, Dalian University, 116622, Dalian, China

      Kedong Ma

    2. Key Laboratory of Microbial Resources Collection and Preservation, Institute of Agricultural Resources and Regional Planning, CAAS, 100081, Beijing, China

      Kedong Ma, Huimin Wang, Shan Zhou, Delong Kong, Jie Zhu, Weiwei Dong, Bingqiang Zhao & Zhiyong Ruan

    3. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, 430070, Wuhan, China

      Xiaorong Chen

    4. Biomass Energy Technology Research Centre, Biogas Institute of Ministry of Agriculture, 610041, Chengdu, China

      Xiang Guo, Yanwei Wang, Mingxiong He & Guoquan Hu

    5. Chinese Academy of Fishery Sciences, 100141, Beijing, China

      Jinlong Song

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    Correspondence to Zhiyong Ruan.

    Additional information

    Communicated by Erko Stackebrandt.

    Kedong Ma, Xiaorong Chen, Xiang Guo have contributed equally to the work and share first authorship.

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    Ma, K., Chen, X., Guo, X. et al. Bacillus vini sp. nov. isolated from alcohol fermentation pit mud. Arch Microbiol 198, 559–564 (2016). https://doi.org/10.1007/s00203-016-1218-4

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    • DOI: https://doi.org/10.1007/s00203-016-1218-4

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