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Genetic analysis of the phosphinothricin-tripeptide biosynthetic pathway of Streptomyces viridochromogenes Tü494

  • Applied Genetics and Regulation
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Summary

Streptomyces viridochromogenes Tü494 produces the antibiotic phosphinothricyl-alanyl-alanine (Ptt). Ptt-non-producing mutants were isolated following N-methyl-N′-nitro-N-nitrosoguanidine (NTG) or UV light treatment of spore suspensions. In co-synthesis and bioconversion experiments the mutational blocks in the biosynthetic pathway were localized. The mutant NTG1 was analysed in detail. This mutant acts as a secretor for all other mutants. From bioconversion experiments with presumptive precursors circumstantial evidence was obtained that NTG1 is mutated in a gene involved in the alanylation of N-acetyl-demethyl-phosphinothricin. Using a cosmid gene library the DNA region complementing the defective gene of mutant NTG1 was isolated on a 4-kb BamHI fragment. Subcloning experiments showed that a 3-kb BglII/BamHI fragment is sufficient for complementation of mutant NTG1.

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Author notes

  1. Susanne Talay

    Present address: Gesellschaft für biotechnologische Forschung, Mascheroder Weg 1, D-3300, Braunschweig, Federal Republic of Germany

Authors and Affiliations

  1. Lehrstuhl für Genetik, Universität Bielefeld, Postfach 86 40, D-4800, Bielefeld 1, Federal Republic of Germany

    Renate Alijah, Johannes Dorendorf, Susanne Talay, Alfred Pühler & Wolfgang Wohlleben

Authors
  1. Renate Alijah
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  2. Johannes Dorendorf
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  3. Susanne Talay
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  4. Alfred Pühler
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  5. Wolfgang Wohlleben
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Additional information

Formerly Susanne Müller

Offprint requests to: W. Wohlleben

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Alijah, R., Dorendorf, J., Talay, S. et al. Genetic analysis of the phosphinothricin-tripeptide biosynthetic pathway of Streptomyces viridochromogenes Tü494. Appl Microbiol Biotechnol 34, 749–755 (1991). https://doi.org/10.1007/BF00169345

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  • DOI: https://doi.org/10.1007/BF00169345

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