Abstract
Two recombinants of alkaliphilic Bacillus subtilis LOCK 1086, constructed via different strategies such as cloning the gene encoding bacterial hemoglobin from Vitreoscilla stercoraria (vhb) and overexpression of the gene encoding acetoin reductase/2,3-butanediol dehydrogenase (bdhA) from B. subtilis LOCK 1086, did not produce more 2,3-butanediol (2,3-BD) than the parental strain. In batch fermentations, this strain synthesized 9.46 g/L in 24 h and 12.80 g/L 2,3-BD in 46 h from sugar beet molasses and an apple pomace hydrolysate, respectively. 2,3-BD production by B. subtilis LOCK 1086 was significantly enhanced in fed-batch fermentations. The highest 2,3-BD concentration (75.73 g/L in 114 h, productivity of 0.66 g/L × h) was obtained in the sugar beet molasses–based medium with four feedings with glucose. In a medium based on the apple pomace hydrolysate with three feedings with sucrose, B. subtilis LOCK 1086 produced up to 51.53 g/L 2,3-BD (in 120 h, productivity of 0.43 g/L × h).
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Acknowledgments
Presented results were obtained within the scope of an international ERA-IB project (ERA-NET IB/02/2009) with an acronym PUBB. Financial support was provided by the Polish National Center for Research and Development (NCBiR).
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Białkowska, A.M., Jędrzejczak-Krzepkowska, M., Gromek, E. et al. Effects of genetic modifications and fermentation conditions on 2,3-butanediol production by alkaliphilic Bacillus subtilis . Appl Microbiol Biotechnol 100, 2663–2676 (2016). https://doi.org/10.1007/s00253-015-7164-2
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DOI: https://doi.org/10.1007/s00253-015-7164-2