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A study of N- and P-dependence of nikkomycin production in continuous culture with immobilized cells

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Summary

The influence of nitrogen and phosphate on the biosynthesis of nikkomycin was studied in chemically defined medium. Cells of Streptomyces tendae were immobilized on porous glass particles in a fluidized-bed reactor for continuous production of nikkomycin. Phosphate had no significant influence on the biosynthesis of nikkomycin. However, even a very low concentration of phosphate in the production medium (00.0125 mmol/l) resulted in microbial growth on the particles. The concentration of nitrogen was highly effective in the regulation of the biosynthesis of nikkomycin. A high level of antibiotic production (maximum 3.05 mg/g dry cell weight per hour) was maintained for a period of about 200 h in a medium that contained nitrogen at a concentration of 0.2 g NH4NO3/l.

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Authors and Affiliations

  1. Lehrstuhl für Bioprozesstechnik, Universität Stuttgart, Nobelstrasse 12, D-7000, Stuttgart 80, Federal Republic of Germany

    H. Uwe Trück & Horst Chmiel

  2. Institut für Lebensmitteltechnologie, Universität Hohenheim, Garbenstrasse 25, D-7000, Stuttgart 70, Federal Republic of Germany

    Walter P. Hammes

  3. Fraunhofer-Institut für Grenzflächen- und Bioverfahrenstechnik, Nobelstrasse 12, D-7000, Stuttgart 80, Federal Republic of Germany

    Walter Trösch

Authors
  1. H. Uwe Trück
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  2. Horst Chmiel
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  3. Walter P. Hammes
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  4. Walter Trösch
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Trück, H.U., Chmiel, H., Hammes, W.P. et al. A study of N- and P-dependence of nikkomycin production in continuous culture with immobilized cells. Appl Microbiol Biotechnol 33, 139–144 (1990). https://doi.org/10.1007/BF00176514

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  • DOI: https://doi.org/10.1007/BF00176514

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