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Taxonomy and chemically semi-defined media for the analysis of the tacrolimus producer ‘Streptomyces tsukubaensis’

  • Applied Microbial and Cell Physiology
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Abstract

‘Streptomyces tsukubaensis’ was the first tacrolimus producer strain identified. Although it has been included in the Streptomyces genus, its taxonomic position has not been rigorously determined. By using a polyphasic approach, we have established that the tacrolimus producer strain ‘S. tsukubaensis’ NRRL 18488 represents a unique species in the Streptomyces genus, which is phylogenetically distant from other subsequently described producers. This fact means a horizontal transference of the tacrolimus-producing gene cluster. Physiology, nutrient requirement, and molecular genetics analyses of tacrolimus biosynthesis in ‘S. tsukubaensis’ necessitate chemically defined or semi-defined media, which work as a jigsaw puzzle and allow for pieces (nutrients) exchange. To date, studies related to ‘S. tsukubaensis’ have been mainly focused in the improvement of tacrolimus production using complex industrial fermentation media, which difficulty allows testing of tacrolimus overproduction enhancers or inhibitors because of the presence of non‐defined substances. In the present work, two semi-defined media were developed in order to study the main factors involved in tacrolimus production in ‘S. tsukubaensis’.

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Acknowledgments

This work was supported by a grant of the CICYT Ministry of Innovation and Science (Madrid, Spain) [BIO2006-14853-C02-01 (CONSOLIDER)] and by the European Union through an ERA-IB (PIM2010EEI-00677) international cooperation project. M. Martínez-Castro received a PFU fellowship of the Ministry of Education and Science. C. Barreiro was supported by the European Union program ERA-IB [BioProChemBB project (EIB.08.008)]. We acknowledge the scientific assistance of S. Albillos and the excellent technical support of B. Martín, J. Merino, A. Casenave, and A. Mulero (INBIOTEC).

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Authors and Affiliations

  1. Instituto de Biotecnología de León (INBIOTEC), Parque Científico de León, Avda. Real, no. 1, 24006, León, Spain

    Miriam Martínez-Castro, Zahra Salehi-Najafabadi & Carlos Barreiro

  2. Área de Microbiología, Departamento de Biología Molecular, Universidad de León, Campus de Vegazana s/n, 24071, León, Spain

    Juan Francisco Martín

  3. Instituto Biomar, S.A., Parque Tecnológico de León, Armunia, León, 24009, Spain

    Francisco Romero, Rodrigo Pérez-Sanchiz & Rosa Isabel Fernández-Chimeno

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  1. Miriam Martínez-Castro
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  2. Zahra Salehi-Najafabadi
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  3. Francisco Romero
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Correspondence to Carlos Barreiro.

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Martínez-Castro, M., Salehi-Najafabadi, Z., Romero, F. et al. Taxonomy and chemically semi-defined media for the analysis of the tacrolimus producer ‘Streptomyces tsukubaensis’ . Appl Microbiol Biotechnol 97, 2139–2152 (2013). https://doi.org/10.1007/s00253-012-4364-x

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