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Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose

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Abstract

Cells of the fungus Aureobasidium pullulans ATCC 42023 were immobilized by adsorption on the ion-exchange resin ECTEOLA (epichlorohydrin triethanolamine)-cellulose and the immobilized cells were examined for their ability to produce the polysaccharide pullulan using batch fermentation. It was found that the cells immobilized on the ECTEOLA-cellulose at pH 2.0 produced higher pullulan levels than those cells immobilized at pH 3.0, 4.0, 5.0, 6.0 and 7.0 after 72 h at 30°C. The pH 2.0-immobilized cells were capable of producing pullulan for 2 cycles of 168 h. Pullulan production by the immobilized cells decreased slightly during the second production cycle but the difference in production was not statistically significant after 168 h.

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Acknowledgements

Financial support of this work was provided by the South Dakota Agricultural Experiment Station and USDA Grant No. 94-37501-0884. The technical assistance of Beth Nemmers was greatly appreciated.

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  1. Department of Biology and Microbiology, South Dakota State University, Brookings, SD, 57007, USA

    Thomas P. West

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  1. Thomas P. West
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Correspondence to Thomas P. West.

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West, T.P. Pullulan production by Aureobasidium pullulans cells immobilized on ECTEOLA-cellulose. Ann Microbiol 60, 763–766 (2010). https://doi.org/10.1007/s13213-010-0115-3

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  • DOI: https://doi.org/10.1007/s13213-010-0115-3

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