In understudied regions of the world, beekeeper records can provide valuable insights into changes in pollinator population trends. We conducted a questionnaire survey of 116 beekeepers in a mountainous area of Western Nepal, where the native honeybee Apis cerana cerana is kept as a managed bee. We complemented the survey with field data on insect–crop visitation, a household income survey, and an interview with a local lead beekeeper. In total, 76% of beekeepers reported declines in honeybees, while 86% and 78% reported declines in honey yield and number of beehives, respectively. Honey yield per hive fell by 50% between 2012 and 2022, whilst the number of occupied hives decreased by 44%. Beekeepers ranked climate change and declining flower abundance as the most important drivers of the decline. This raises concern for the future food and economic security of this region, where honey sales contribute to 16% of total household income, and where Apis cerana cerana plays a major role in crop pollination, contributing more than 50% of all flower visits to apple, cucumber, and pumpkin. To mitigate further declines, we promote native habitat and wildflower preservation, and using well-insulated log hives to buffer bees against the increasingly extreme temperature fluctuations. Full article

In the context of carbon neutrality and carbon peaking, in order to achieve low carbon emissions and promote the efficient utilization of wind energy, hydrogen energy as an important energy carrier is proposed to mix hydrogen and natural gas to form hydrogen-enriched compressed natural gas (HCNG). It is also injected into the natural gas pipeline network to achieve the transmission and utilization of hydrogen energy. At the same time, the participation of demand response is considered, the load’s peak and trough periods are adjusted, and the large-scale consumption of renewable energy and the reduction in carbon emissions are achieved. First of all, a fine model of hydrogen production and hydrogen use equipment is established to analyze the impact of adding hydrogen mixing on the economy and the low-carbon property of the system. With green certificates and demand response, the utilization rate of hydrogen energy is improved to further explore the energy utilization rate and emission reduction capacity of the system. Secondly, on the basis of modeling, the optimal scheduling strategy is proposed with the sum of energy purchase cost, equipment operation cost, carbon emission cost, wind curtailment cost, and green certificate income as the lowest objective function. Considering the constraints such as hydrogen blending ratio and flexible load ratio of the pipeline network, a low-carbon economic scheduling model of hydrogen mixed natural gas was established. The model was linearized and solved by using MATLAB 2021a and CPLEX solver. By comparing different scenarios, the superiority of the model and the effectiveness of the strategy are verified. Full article

In the present work, the development of geopolymeric materials with Na or K based on industrial kaolin samples, with variable kaolinite content and alkaline silicates, is studied. XRF, XRD, FTIR and SEM-EDS have been used as characterization techniques. Three ceramic kaolin samples, two from Algeria and one from Charente (France), have been considered. In particular, chemical and mineralogical characterization revealed elements distinct of Si and Al, and the content of pure kaolinite and secondary minerals. Metakaolinite was obtained by grinding and sieving raw kaolin at 80 μm and then by thermal activation at 750 °C for 1 h. This metakaolinite has been used as a base raw material to obtain geopolymers, using for this purpose different formulations of alkaline silicates with NaOH or KOH and variable Si/K molar ratios. The formation of geopolymeric materials by hydroxylation and polycondensation characterized with different Si/Al molar ratios, depending on the original metakaolinite content, has been demonstrated. Sodium carbonates have been detected by XRD and FTIR, and confirmed by SEM-EDS, in two of these geopolymer materials being products of NaOH carbonation. Full article

In the context of our ongoing studies on chromane derivatives as inhibitors of the salicylate synthase from M. tuberculosis, we isolated a new, unexpected compound from the cyclization of 3-(propargyloxy)-5-benzyloxy-benzoic acid methyl ester. Its molecular structure was elucidated by means of 1D and 2D NMR analyses, FT-IR, ESI-MS, and HRMS. Full article

Overage construction workers have received widespread attention due to the higher safety risk. The balance of interests among the contractor, supervisor, and regulator forms a game, whose dynamics are tightly linked to the contractor’s risk perception. This study aims to construct a tripartite evolutionary game model with risk perception integrated, thus proposing tailored supervisory strategies for supervisors and regulators. Unlike the traditional scale-based approach, a behavior-based method is developed to measure the contractor’s risk perception, which improves the interpretability of results and avoids complex questionnaire surveys. The simulation results reveal a clear correlation between the contractor’s risk perception and behavior. It is recommended that supervisors increase penalties and the initial possibility of Rigorous Inspection properly, and regulators may consider enhancing credit-based future returns and losses. Notably, certain measures may not be applicable to all contractors. Hence, supervisors and regulators should identify their risk perception before adopting supervisory strategies. Full article

This paper aims to propose an quantitative engineering approach to study and compare the jaw mechanisms of different marine species, considering essential mechanical parameters generally used to evaluate the performance of industrial linkage mechanisms. By leveraging these parameters, the paper demonstrates how the species’ characteristics and behaviors align with the findings of biologists, enabling a meaningful comparison that was not previously possible. Seven fish species from various families are chosen to maintain a generic approach. Full article

Antimicrobial resistance is a silent pandemic harming human health, and Pseudomonas aeruginosa is the most common bacterium responsible for chronic pulmonary and eye infections. Antimicrobial peptides (AMPs) represent promising alternatives to conventional antibiotics. In this review, the in vitro/in vivo activities of the frog skin-derived AMP Esc(1-21) are shown. Esc(1-21) rapidly kills both the planktonic and sessile forms of P. aeruginosa and stimulates migration of epithelial cells, likely favoring repair of damaged tissue. However, to undertake preclinical studies, some drawbacks of AMPs (cytotoxicity, poor biostability, and limited delivery to the target site) must be overcome. For this purpose, the stereochemistry of two amino acids of Esc(1-21) was changed to obtain the diastereomer Esc(1-21)-1c, which is more stable, less cytotoxic, and more efficient in treating P. aeruginosa-induced lung and cornea infections in mouse models. Incorporation of these peptides (Esc peptides) into nanoparticles or immobilization to a medical device (contact lens) was revealed to be an effective strategy to ameliorate and/or to prolong the peptides’ antimicrobial efficacy. Overall, these data make Esc peptides encouraging candidates for novel multifunctional drugs to treat lung pathology especially in patients with cystic fibrosis and eye dysfunctions, characterized by both tissue injury and bacterial infection. Full article

Experiences of distress and challenging emotions in response to the climate crisis are increasingly common, particularly among young adults. These experiences can include challenging emotions caused by the harmful environmental impacts of conventional food systems, as their contributions to greenhouse gas emissions have become more widely known. While recent studies have examined various experiences of climate distress, the interaction between climate distress and food practice remains poorly understood. In this research, we turn to this intersection by examining the experiences of climate distress of young adults who have alternative food practices, and the interaction between their climate distress and their alternative food practices. Guided by an exploratory, single case study research approach, this research draws from 20 semi-structured interviews conducted with young adults located in urban centres in the Southeastern Prairie Region of Canada. Thematic analysis of the findings reveals that participants experience a variety of climate emotions and a personal responsibility to act in response to the climate crisis. The findings suggest that because of their ability to facilitate a meaningful and practical environmental impact, alternative food practices represent significant climate actions and may be pathways to facilitate coping or managing climate distress among young adults. Results demonstrate the psychological impacts of the climate crisis on young adults, highlighting the need for action on climate change and climate distress. Increasing the accessibility of alternative food practices may support young adults in coping with challenging climate emotions. Full article

In the field of robotic automation, achieving high position accuracy in robotic vision systems (RVSs) is a pivotal challenge that directly impacts the efficiency and effectiveness of industrial applications. This study introduces a comprehensive modeling approach that integrates kinematic and joint compliance factors to significantly enhance the position accuracy of a system. In the first place, we develop a unified kinematic model that effectively reduces the complexity and error accumulation associated with the calibration of robotic systems. At the heart of our approach is the formulation of a joint compliance model that meticulously accounts for the intricacies of the joint connector, the external load, and the self-weight of robotic links. By employing a novel 3D rotary laser sensor for precise error measurement and model calibration, our method offers a streamlined and efficient solution for the accurate integration of vision systems into robotic operations. The efficacy of our proposed models is validated through experiments conducted on a FANUC LR Mate 200iD robot, showcasing notable improvements in the position accuracy of robotic vision system. Our findings contribute a framework for the calibration and error compensation of RVS, holding significant potential for advancements in automated tasks requiring high precision. Full article

Several studies have shown a relation between obesity and cognitive decline, highlighting a significant global health challenge. In recent years, artificial intelligence (AI) and machine learning (ML) have been integrated into clinical practice for analyzing datasets to identify new risk factors, build predictive models, and develop personalized interventions, thereby providing useful information to healthcare professionals. This systematic review aims to evaluate the potential of AI and ML techniques in addressing the relationship between obesity, its associated health consequences, and cognitive decline. Methods: Systematic searches were performed in PubMed, Cochrane, Web of Science, Scopus, Embase, and PsycInfo databases, which yielded eight studies. After reading the full text of the selected studies and applying predefined inclusion criteria, eight studies were included based on pertinence and relevance to the topic. Results: The findings underscore the utility of AI and ML in assessing risk and predicting cognitive decline in obese patients. Furthermore, these new technology models identified key risk factors and predictive biomarkers, paving the way for tailored prevention strategies and treatment plans. Conclusions: The early detection, prevention, and personalized interventions facilitated by these technologies can significantly reduce costs and time. Future research should assess ethical considerations, data privacy, and equitable access for all. Full article

Emmental or Swiss cheese is a hard, ripened cheese appreciated by consumers for its appearance and taste. This study aimed to investigate the profile of Swiss cheese consumers and producers from Ţara Dornelor area, Romania, along with the sensory analysis of the Dorna Swiss cheese produced there. For this purpose, a questionnaire was applied to 268 participants to evaluate consumer behavior. Consumers were grouped depending on consumption frequency (low—once or a few times a year, medium—once a month, and high—once a week or more), and the behavior of groups was evaluated. Producer opinion was assessed by interview and Swiss cheese sensory characteristics in two seasons were determined by sensory analysis using a semi-trained panel. The results showed that the main factors affecting consumer purchase decision are the ingredients (4.43), taste and flavor (4.41), appearance and texture (4.23), producer (3.98), nutritional value (3.88), and product history (3.67). Clustering of consumers depending on consumption frequency revealed significant differences (p < 0.05) regarding the purchase place and some factors influencing the purchase decision such as price, health benefits, and nutritional value. Producers asserted that the quality of milk is the main problem in Swiss cheese production. They consider that the raw material quality and origin, hygiene, utilities, and legislation have the greatest impact on the production process, while the trading is mainly affected by the product taste and flavor, appearance and texture, quality label, price, and product history. The sensory characteristics differed significantly (p < 0.05) between producers and seasons, with the sample produced in a stainless-steel tank and without exogenous microflora being the most appreciated in summer. These results could help producers adapt their product quality and marketing policy to consumer preferences. Full article

Comparison of the ⁸⁷Sr/⁸⁶Sr signatures of archaeological osteological material with features of geological provinces can be applied to determine the places of birth and living of individuals. Such reconstructions were conducted for both humans and domestic animals at the Late Bronze–Early Iron Age sites of the Crimea. The Crimean Peninsula is an interesting testing polygon for such research because it is characterized by a diverse geological situation within a relatively small area. The initial data allowed us to distinguish between three groups of mobility at the Bai-Kiyat I settlement and two groups at the Dolgii Bugor site. The Bai-Kiyat I site is located on the seacoast, so the proxy line for this area will correspond to the value of the ratio of strontium isotopes in seawater (0.7092). The inhabitants of this settlement, including a child from a burial on the settlement, are characterized by this value of strontium isotopes. Other groups include nonlocal people. The data obtained indicate that the steppe zone of the Northern Black Sea region was an ecumene, within which active mobility of groups of people was registered. This mobility is associated primarily with the pastoral type of economy in the period from the Chalcolithic to the Early Iron Age. Full article

Historical news media reports serve as a vital data source for understanding the risk of urban ground collapse (UGC) events. At present, the application of large language models (LLMs) offers unprecedented opportunities to effectively extract UGC events and their spatiotemporal information from a vast amount of news reports and media data. Therefore, this study proposes an LLM-based inventory construction framework consisting of three steps: news reports crawling, UGC event recognition, and event attribute extraction. Focusing on Zhejiang province, China, as the test region, a total of 27 cases of collapse events from 637 news reports were collected for 11 prefecture-level cities. The method achieved a recall rate of over 60% and a precision below 35%, indicating its potential for effectively and automatically screening collapse events; however, the accuracy needs to be improved to account for confusion with other urban collapse events, such as bridge collapses. The obtained UGC event inventory is the first open access inventory based on internet news reports, event dates and locations, and collapse co-ordinates derived from unstructured contents. Furthermore, this study provides insights into the spatial pattern of UGC frequency in Zhejiang province, effectively supplementing the statistical data provided by the local government. Full article

Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) is able to infect many economically important crops and thus causes substantial losses in the global agricultural economy. Pst DC3000 can be divided into virulent lines and avirulent lines. For instance, the pathogen effector avrRPM1 of avirulent line Pst-avrRpm1 (Pst DC3000 avrRpm1) can be recognized and detoxified by the plant. To further compare the pathogenicity mechanisms of virulent and avirulent Pst DC3000, a comprehensive analysis of the acetylome and succinylome in Arabidopsis thaliana was conducted following infection with virulent line Pst DC3000 and avirulent line Pst-avrRpm1. In this study, a total of 1625 acetylated proteins encompassing 3423 distinct acetylation sites were successfully identified. Additionally, 229 succinylated proteins with 527 unique succinylation sites were detected. A comparison of these modification profiles between plants infected with Pst DC3000 and Pst-avrRpm1 revealed significant differences. Specifically, modification sites demonstrated inconsistencies, with a variance of up to 10% compared to the control group. Moreover, lysine acetylation (Kac) and lysine succinylation (Ksu) displayed distinct preferences in their modification patterns. Lysine acetylation is observed to exhibit a tendency towards up-regulation in Arabidopsis infected with Pst-avrRpm1. Conversely, the disparity in the number of Ksu up-regulated and down-regulated sites was not as pronounced. Motif enrichment analysis disclosed that acetylation modification sequences are relatively conserved, and regions rich in polar acidic/basic and non-polar hydrophobic amino acids are hotspots for acetylation modifications. Functional enrichment analysis indicated that the differentially modified proteins are primarily enriched in the photosynthesis pathway, particularly in relation to light-capturing proteins. In conclusion, this study provides an insightful profile of the lysine acetylome and succinylome in A. thaliana infected with virulent and avirulent lines of Pst DC3000. Our findings revealed the potential impact of these post-translational modifications (PTMs) on the physiological functions of the host plant during pathogen infection. This study offers valuable insights into the complex interactions between plant pathogens and their hosts, laying the groundwork for future research on disease resistance and pathogenesis mechanisms. Full article

This paper introduces an analog integrated fractional order type-2 fuzzy PID control system. Current approaches frequently depend on energy-intensive embedded digital systems, consuming substantial energy levels ranging from a few µW to mW. To address this limitation we propose a fully analog design offering insights into the potential of analog circuits for power-efficient robust control in complex and uncertain environments. It consists of Gaussian function, min/max, Operational transcoductance amplifier circuits and Resistor-Capacitor networks for the implementation of the fractional-order components. Crafted for operation under a reduced voltage supply (0.6 V), the controller attains minimal power usage (861.8 nW), facilitating uninterrupted, extended-term functioning. Post-layout simulation results confirm the proper operation of the proposed design. The proposed system is designed and simulated using the Cadence IC Suite in a TSMC 90 nm CMOS process. Full article

According to Bronfenbrenner’s ecological theory, the interaction between micro- and macro-factors improves human development. Previous studies suggest that community social capital, which refers to the interaction between residents and communities, promotes the subjective well-being of residents in residential communities. However, little is known about the mechanisms underlying this relationship. This study explored the link between community social capital and life satisfaction among urban residents and examined the mediating role of psychological flourishing and moderating effect of educational level. Data were obtained from a cross-community survey of 816 urban residents in China aged 20–60 years. The results indicated that psychological flourishing mediated the link between community social capital and life satisfaction among urban residents after controlling for gender, age, marital status, dwelling time, and monthly income. Furthermore, educational attainment moderated the association between community social capital and psychological flourishing. The mediating effect of psychological flourishing was stronger among residents with low educational attainment than those with high educational attainment, indicating that the benefits of community social capital were greater among the former group. This study extends our knowledge of the effect of community social capital on urban residents’ subjective well-being, including its role in reducing educational inequality in human well-being. Full article

To study the spatiotemporal variability of particle-bound polycyclic aromatic hydrocarbons (PAHs) and assess their carcinogenic potential in six contrasting urban environments in Greece, a total of 305 filter samples were collected and analyzed. Sampling sites included a variety of urban background, traffic (Athens, Ioannina and Heraklion), rural (Xanthi) and near-port locations (Piraeus and Volos). When considering the sum of 16 U.S. EPA priority PAHs, as well as that of the six EU-proposed members, average concentrations observed across locations during summer varied moderately (0.4–2.2 ng m⁻³) and independently of the population of each site, with the highest values observed in the areas of Piraeus and Volos that are affected by port and industrial activities. Winter levels were significantly higher and more spatially variable compared to summer, with the seasonal enhancement ranging from 7 times in Piraeus to 98 times in Ioannina, indicating the large impact of PAH emissions from residential wood burning. Regarding benzo(a)pyrene (BaP), an IARC Group 1 carcinogen and the only EU-regulated PAH, the winter/summer ratios were 24–33 in Athens, Volos, Heraklion and Xanthi; 60 in Piraeus; and 480 in Ioannina, which is afflicted by severe wood-burning pollution events. An excellent correlation was observed between organic carbon (OC) and benzo(a)pyrene (BaP) during the cold period at all urban sites (r² > 0.8) with stable BaP/OC slopes (0.09–0.14 × 10⁻³), highlighting the potential use of OC as a proxy for the estimation of BaP in winter conditions. The identified spatiotemporal contrasts, which were explored for the first time for PAHs at such a scale in the Eastern Mediterranean, provide important insights into sources and controlling atmospheric conditions and reveal large deviations in exposure risks among cities that raise the issue of environmental injustice on a national level. Full article

To date, three carbapenem resistance mechanisms have been identified: carbapenemase released from the pathogen, changes in the expression of the outer membrane OprD porin, and overexpression of the efflux pump MexAB-OprM. Twelve carbapenemase-negative carbapenem-resistant Pseudomonas aeruginosa strains, isolated from patients hospitalized at the University Hospital of Larissa, Central Greece, during 2023, which belonged to various sequence types (STs), were selected and were studied focusing on the characterization of their β-lactamases, on changes to OprD and its regulator MexT proteins, and on alterations to the MexAB-OprM regulator proteins encoded by the mexR, nalC, and nalD genes. Whole genome sequencing analysis revealed the presence of β-lactamase encoding genes, with bla_PAO present in all isolates. Additionally, seven different genes of the oxacillinase family (bla_OXA-35, bla_OXA-50, bla_OXA-395, bla_OXA-396, bla_OXA-486, bla_OXA-488, bla_OXA-494) were identified, with each strain harboring one to three of these. Regarding the OprD, five strains had truncated structures, at Loop 2, Loop 3, Loop 4, and Loop 9, while the remaining strains carried previously reported amino acid changes. Further, an additional strain had a truncated MexR; whereas, two other strains had totally modified NalC sequences. The active form of MexT, responsible for the downregulation of OprD production, as the intact sequence of the NalD protein, was found in all the strains studied. It is concluded that the truncated OprD, MexR, and NalC proteins, detected in eight strains, probably led to inactive proteins, contributing to carbapenem resistance. However, four strains carried known modifications in OprD, MexR, and NalC, as previously reported in both susceptible and resistant strains, a finding that indicates the complexity of carbapenem resistance in P. aeruginosa. Full article

Wireless sensor networks have gained significant attention in recent years due to their wide range of applications in environmental monitoring, surveillance, and other fields. The design of a groundwater quality and quantity monitoring network is an important aspect in aquifer restoration and the prevention of groundwater pollution and overexploitation. Moreover, the development of a novel localization strategy project in wireless sensor groundwater networks aims to address the challenge of optimizing sensor location in relation to the monitoring process so as to extract the maximum quantity of information with the minimum cost. In this study, the improved hybrid butterfly artificial gorilla troop optimizer (iHBAGTO) technique is applied to optimize nodes’ position and the analysis of the path loss delay, and the RSS is calculated. The hybrid of Butterfly Artificial Intelligence and an artificial gorilla troop optimizer is used in the multi-functional derivation and the convergence rate to produce the designed data localization. The proposed iHBAGTO algorithm demonstrated the highest convergence rate of 99.6%, and it achieved the lowest average error of 4.8; it consistently had the lowest delay of 13.3 ms for all iteration counts, and it has the highest path loss values of 8.2 dB, with the lowest energy consumption value of 0.01 J, and has the highest received signal strength value of 86% for all iteration counts. Overall, the Proposed iHBAGTO algorithm outperforms other algorithms. Full article

While neutralizing antibodies (nAbs) induced by monovalent severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) vaccinations are primarily directed against the wildtype (WT), subsequent exposure to the Omicron variants may increase the breadth of the antibodies´ cross-neutralizing activity. Here, we analyzed the impact of an Omicron breakthrough infection (BTI) or a fourth monovalent mRNA vaccination on nAb profiles in people living with human immunodeficiency virus (PLWH). Using a multivariant surrogate virus neutralization test (sVNT), we quantified nAbs in 36 three-times vaccinated PLWH, of whom 9 acquired a serologically confirmed Omicron BTI, 8 received a fourth vaccine dose, and 19 were neither infected nor additionally vaccinated. While nAbs against WT and Delta increased after the BTI and a fourth vaccination, a significant increase against BA.1, BA.2, and BA.5 was only observed after the BTI. However, there was no significant difference in nAb concentrations between the samples obtained after the BTI and fourth vaccination. In contrast, nAb levels were significantly lower in PLWH, who were neither infected nor additionally vaccinated after three vaccinations. Thus, our study demonstrates the suitability of a multivariant sVNT to assess hybrid humoral immunity after Omicron BTIs in PLWH vaccinated against SARS-CoV-2. Full article

Global warming is exacerbating flood hazards, making the robustness of flood risk management a critical issue. Without considering future scenarios, flood risk analysis built only on historical knowledge may not adequately address the coming challenges posed by climate change. A comprehensive risk analysis framework based on both historical inundations and future projections to tackle uncertainty is still lacking. In this view, a scenario-based, data-driven risk analysis framework that for the first time integrates recent historical floods and future risk trends is here presented, consisting of flood inundation-prone and high-risk zones. Considering the Poyang Lake Eco-Economic Zone (PLEEZ) in China as the study area, we reproduced historical inundation scenarios of major flood events by using Sentinel-1 imagery from 2015 to 2021, and used them to build the risk baseline model. The results show that 11.7% of the PLEEZ is currently exposed to the high-risk zone. In the SSP2-RCP4.5 scenario, the risk would gradually decrease after peaking around 2040 (with a 19.3% increase in high-risk areas), while under the traditional fossil fuel-dominated development pathway (SSP5-RCP8.5), the risk peak would occur with a higher intensity about a decade earlier. The attribution analysis results reveal that the intensification of heavy rainfall is the dominant driver of future risk increase and that the exploitation of unused land such as wetlands induces a significant increase in risk. Finally, a hierarchical panel of recommended management measures was developed. We hope that our risk analysis framework inspires newfound risk awareness and provides the basis for more effective flood risk management in river basins. Full article