We investigated the experiences of Brazilian gay men with HIV, focusing on the moment of diagnosis and its potential biopsychosocial impacts. This clinical–qualitative study involved 15 participants interviewed online and synchronously by a clinical psychologist in 2021. Thematic analysis was employed to analyze the data. Interpretations were grounded in Minority Stress Theory. Four thematic axes emerged, including “Diagnostic Revelation”, “Social and Internalized Stigma”, “Biopsychosocial Effects of Living with HIV”, and “Gratitude for Treatment Advances and the Brazilian Health System”. The diagnosis was often experienced as traumatic, exacerbated by the absence of empathy and emotional support from healthcare providers. Participants commonly reported guilt, fear upon learning of their HIV status, social isolation, loneliness, lack of social support, and damage to affective-sexual relationships. Many also noted a decline in mental health, even those without HIV-related medical complications. Despite over 40 years since the HIV epidemic began, the prevalence of homophobia and serophobia among gay men remains widespread, including within the multidisciplinary teams of specialized services. This indicates that the stigma associated with homosexuality and HIV persists, despite significant biomedical progress in the diagnosis and treatment of the infection, particularly in Brazil. Full article

Background: Professional cycling puts significant demands on the respiratory system. Exercise-induced bronchoconstriction (EIB) is a common problem in professional athletes. Small airways may be affected in isolation or in combination with a reduction in forced expiratory volume at the first second (FEV₁). This study aimed to investigate isolated exercise-induced small airway dysfunction (SAD) in professional cyclists and assess the impact of this phenomenon on exercise capacity in this population. Materials and Methods: This research was conducted on professional cyclists with no history of asthma or atopy. Anthropometric characteristics were recorded, the training age was determined, and spirometry and specific markers, such as fractional exhaled nitric oxide (FeNO) and immunoglobulin E (IgE), were measured for all participants. All of the cyclists underwent cardiopulmonary exercise testing (CPET) followed by spirometry. Results: Compared with the controls, 1-FEV₃/FVC (the fraction of the FVC that was not expired during the first 3 s of the FVC) was greater in athletes with EIB, but also in those with isolated exercise-induced SAD. The exercise capacity was lower in cyclists with isolated exercise-induced SAD than in the controls, but was similar to that in cyclists with EIB. This phenomenon appeared to be associated with a worse ventilatory reserve (VE/MVV%). Conclusions: According to our data, it appears that professional cyclists may experience no beneficial impacts on their respiratory system. Strenuous endurance exercise can induce airway injury, which is followed by a restorative process. The repeated cycle of injury and repair can trigger the release of pro-inflammatory mediators, the disruption of the airway epithelial barrier, and plasma exudation, which gradually give rise to airway hyper-responsiveness, exercise-induced bronchoconstriction, intrabronchial inflammation, peribronchial fibrosis, and respiratory symptoms. The small airways may be affected in isolation or in combination with a reduction in FEV₁. Cyclists with isolated exercise-induced SAD had lower exercise capacity than those in the control group. Full article

Peri-implantitis is an inflammatory condition characterized by inflammation in the peri-implant connective tissue and a progressive loss of supporting bone; it is commonly associated with the presence of biofilms on the surface of the implant, which is an important factor in the development and progression of the disease. The objective of this study was to evaluate, using micro-CT, the bone regeneration of surgically created peri-implant defects exposed to a microcosm of peri-implantitis. Twenty-three adult New Zealand white rabbits were included in the study. Bone defects of 7 mm diameter were created in both tibiae, and a cap-shaped titanium device was placed in the center, counter-implanted with a peri-implantitis microcosm. The bone defects received a bone substitute and/or a resorbable synthetic PLGA membrane, according to random distribution. Euthanasia was performed 15 and 30 days postoperatively. Micro-CT was performed on all samples to quantify bone regeneration parameters. Bone regeneration of critical defects occurred in all experimental groups, with a significantly greater increase in cases that received bone graft treatment (p < 0.0001), in all measured parameters, at 15 and 30 days. No significant differences were observed in the different bone neoformation parameters between the groups that did not receive bone grafts (p > 0.05). In this experimental model, the presence of peri-implantitis microcosms was not a determining factor in the bone volume parameter, both in the groups that received regenerative treatment and in those that did not. Full article

High-grade serous ovarian cancer (HGSOC) is the predominant subtype of ovarian cancer (OC), occurring in more than 80% of patients diagnosed with this malignancy. Histological and genetic analysis have confirmed the secretory epithelial of the fallopian tube (FT) as a major site of origin of HGSOC. Although there have been significant strides in our understanding of this disease, early stage detection and diagnosis are still rare. Current clinical imaging modalities lack the ability to detect early stage pathogenesis in the fallopian tubes and the ovaries. However, there are several microscopic imaging techniques used to analyze the structural modifications in the extracellular matrix (ECM) protein collagen in ex vivo FT and ovarian tissues that potentially can be modified to fit the clinical setting. In this perspective, we evaluate and compare the myriad of optical tools available to visualize these alterations and the invaluable insights these data provide on HGSOC initiation. We also discuss the clinical implications of these findings and how these data may help novel tools for early diagnosis of HGSOC. Full article

In order to evaluate the damage to tunnel cables caused by fire caused by leakage of transformer oil into a cable channel, the fire characteristics of different volumes of transformer oil flowing into a cable channel were analyzed by numerical simulation. The results show that when the total leakage of transformer oil is less than or equal to 3 L, the fire will end within 120 s, and when the total leakage is greater than or equal to 5 L, the fire duration will exceed 900 s. When the leakage amount is 1 L, the cable only burns slightly, and when the leakage amount is 3~12 L, the cable burns obviously. The combustion of the cable is mainly concentrated between 15 s and 75 s, and the overall combustion rate of the cable increases first and then decreases. When the total leakage is greater than or equal to 8 L, the damage distance of the middle and lower layer cable is the smallest. When the total leakage is less than or equal to 5 L, the damage distance of the lower layer cable is the smallest, and the damage distance of the lower layer cable, middle and lower layer cable, and middle and upper layer cable is less than half of the length of the cable channel. Full article

Plastic pollution poses a significant environmental challenge, necessitating the investigation of bioplastics with reduced end-of-life impact. This study systematically characterizes four promising bioplastics—polybutylene adipate terephthalate (PBAT), polybutylene succinate (PBS), poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and polylactic acid (PLA). Through a comprehensive analysis of their chemical, thermal, and mechanical properties, we elucidate their structural intricacies, processing behaviors, and potential morphologies. Employing an environmentally friendly process utilizing supercritical carbon dioxide, we successfully produced porous materials with microcellular structures. PBAT, PBS, and PLA exhibit closed-cell morphologies, while PHBV presents open cells, reflecting their distinct overall properties. Notably, PBAT foam demonstrated an average porous area of 1030.86 μm², PBS showed an average porous area of 673 μm², PHBV displayed open pores with an average area of 116.6 μm², and PLA exhibited an average porous area of 620 μm². Despite the intricacies involved in correlating morphology with material properties, the observed variations in pore area sizes align with the findings from chemical, thermal, and mechanical characterization. This alignment enhances our understanding of the morphological characteristics of each sample. Therefore, here, we report an advancement and comprehensive research in bioplastics, offering deeper insights into their properties and potential morphologies with an easy sustainable foaming process. The alignment of the process with sustainability principles, coupled with the unique features of each polymer, positions them as environmentally conscious and versatile materials for a range of applications. Full article

In the context of autonomous driving, the fusion of LiDAR and camera sensors is essential for robust obstacle detection and distance estimation. However, accurately estimating the transformation matrix between cost-effective low-resolution LiDAR and cameras presents challenges due to the generation of uncertain points by low-resolution LiDAR. In the present work, a new calibration technique is developed to accurately transform low-resolution 2D LiDAR points into camera pixels by utilizing both static and dynamic calibration patterns. Initially, the key corresponding points are identified at the intersection of 2D LiDAR points and calibration patterns. Subsequently, interpolation is applied to generate additional corresponding points for estimating the homography matrix. The homography matrix is then optimized using the Levenberg–Marquardt algorithm to minimize the rotation error, followed by a Procrustes analysis to minimize the translation error. The accuracy of the developed calibration technique is validated through various experiments (varying distances and orientations). The experimental findings demonstrate that the developed calibration technique significantly reduces the mean reprojection error by 0.45 pixels, rotation error by 65.08%, and distance error by 71.93% compared to the standard homography technique. Thus, the developed calibration technique promises the accurate transformation of low-resolution LiDAR points into camera pixels, thereby contributing to improved obstacle perception in intelligent autonomous driving systems. Full article

Background: Precision oncology treatments are being applied more commonly in breast and gynecological oncology through the implementation of Molecular Tumor Boards (MTBs), but real-world clinical outcome data remain limited. Methods: A retrospective analysis was conducted in patients with breast cancer (BC) and gynecological malignancies referred to our center’s MTB from 2018 to 2023. The analysis covered patient characteristics, next-generation sequencing (NGS) results, MTB recommendations, therapy received, and clinical outcomes. Results: Sixty-three patients (77.8%) had metastatic disease, and forty-four patients (54.3%) had previously undergone three or more lines of systemic treatment. Personalized treatment recommendations were provided to 50 patients (63.3%), while 29 (36.7%) had no actionable target. Ultimately, 23 patients (29.1%) underwent molecular-matched treatment (MMT). Commonly altered genes in patients with pan-gyn tumors (BC and gynecological malignancies) included TP53 (n = 42/81, 51.9%), PIK3CA (n = 18/81, 22.2%), BRCA1/2 (n = 10/81, 12.3%), and ARID1A (n = 9/81, 11.1%). Patients treated with MMT showed significantly prolonged progression-free survival (median PFS 5.5 vs. 3.5 months, p = 0.0014). Of all patients who underwent molecular profiling, 13.6% experienced a major clinical benefit (PFSr ≥ 1.3 and PR/SD ≥ 6 months) through precision oncology. Conclusions: NGS-guided precision oncology demonstrated improved clinical outcomes in a subgroup of patients with gynecological and breast cancers. Full article

Fused deposition modeling (FDM) is a rather new technology in the production of personalized dosage forms. The melting and printing of polymer–active pharmaceutical ingredient (API)—mixtures can be used to produce oral dosage forms with different dosage as well as release behavior. This process is utilized to increase the bioavailability of pharmaceutically relevant active ingredients that are poorly soluble in physiological medium by transforming them into solid amorphous dispersions (ASD). The release from such ASDs is expected to be faster and higher compared to the raw materials and thus enhance bioavailability. Printing directly from powder while forming ASDs from loperamide in Polyvinylalcohol was realized. Different techniques such as a change in infill and the incorporation of sorbitol as a plastisizer to change release patterns as well as a non-destructive way for the determination of API distribution were shown. By measuring the melt viscosities of the mixtures printed, a rheological model for the printer used is proposed. Full article

The COVID-19 pandemic was a significant disruption to the construction industry around the globe with multiple impacts, such as workforce limitations and contractual conflicts. Multiple studies have explored the impacts of the pandemic in the construction sector so far. However, little is known about how construction companies responded to the pandemic and what companies’ characteristics may have influenced their responses. The objective of this study is to explore the impacts of COVID-19 and how construction companies responded to the pandemic. To do so, semi-structured interviews with experienced professional working in Chile are qualitatively analyzed to leverage their experience. Furthermore, characteristics of construction companies that influenced how companies responded to the pandemic, namely, size, experience, and financial standing, are explored. The results obtained suggest that the focus in responding to the pandemic was taking care of workers’ safety, improving the planning of projects under highly uncertain conditions, and dealing with the financial stress of developing construction projects. When looking at the influence of companies’ experience, size, and economic capacity, experienced and large companies’ responses were related to implementing teleworking and dealing with a limited workforce. Regarding the economic capacity of construction companies, the focus was placed on responding to the pandemic using multiple sources of financing. Differences identified in how construction companies responded to the pandemic emphasizes the importance of understanding attributes that led companies to having better responses to the pandemic and being prepared for the post-pandemic context. Full article

Within the food sector, there is a growing embrace of meat substitutes as a more sustainable alternative to meat, driven by ethical, environmental, and health considerations. This study aims to explore consumer behavior and willingness to pay (WTP) for plant-based meat alternatives (PBMAs), illustrated by the example of vegan burger patties. The sample of the study (n = 433) consists of young consumers roughly below 30 years of age, known as Generation Z (Gen Z). The study aims to (1) assess of the importance of PBMA attributes to Gen Z, and (2) approximate Gen Z’s willingness to pay for specific PBMA attribute levels. A choice-based conjoint analysis was used to assess Gen Z’s preferences for meat substitutes. The findings indicate that the most crucial PBMA attribute is origin, followed by price and the primary vegan ingredient. Notably, Gen Z values domestic and EU-sourced products positively, contrasting with the negative perception of third-country imports. Organic production is associated with a positive part-worth utility, whereas the attribute fat content has almost no impact. Consequently, WTP is approximated to be the highest for products of domestic origin compared to the significant discount required for non-EU origin. All other attribute levels have a much lower impact. Despite sociodemographic variables, the respondents’ eating habits (vegan, vegetarian, etc.) most significantly influence the approximation of the importance of some of the PBMA attributes, in particular price and primary ingredient. Full article

NiFe-layered double hydroxides (NiFe-LDH) have been reported to possess exceptional oxygen evolution reaction (OER) activity. However, maintaining the stability of high activity over a long time remains a critical challenge that needs to be addressed for their practical application. Here, we report a custom-sized deep recombination of 2D graphene oxide with NiFe-LDH (NiFe-LDH/GO/NF) through a simple electrodeposition method that improves OER activity and achieves excellent stability. The excellent performance of the catalyst mainly comes from the three-phase interface and electron transport channel dredged by the three-dimensional structure constructed by the deep composite, which can not only significantly reduce its charge and electron transfer resistance, improving the material conductivity, but it also effectively increases the specific surface area, inhibits aggregation, and exposes rich active sites. In addition, GO with good conductivity not only supports NiFe-LDH well but also increases the heterogeneous interface, putting the NiFe-LDH/GO composites in close contact with Ni foam and increasing the electrocatalytic stability of the NiFe-LDH/GO/NF. The experimental results show that the overpotential of NiFe-LDH/20,000GO/NF is only 295 mV at a current density of 100 mA cm⁻²; the Tafel slope is 52 mV dec⁻¹, and the charge transfer resistance (Rct) is only 0.601 Ω in 1 M KOH. This indicates that GO has excellent potential to assist in constructing geometric and electronic structures of NiFe-LDH in long-term applications. Full article

A recent measure was developed to assess the Quality of Life (QoL) of young people with advanced cancer and is available for parents and professionals (Advance QoL). The present study aimed to elaborate self-reported versions for children and adolescents with advanced cancer. We adopted a four-phase research plan: (1) to elaborate the Advance QoL questionnaire for youth (8–12 and 13–18 years old) with a team of young research partners; (2) to evaluate the understandability of these versions in a sample of 12 young patients from the target population using cognitive interviews; (3) to assess social validity in the same group using a questionnaire and the content validity index (CVI); and (4) to refine the questionnaires according to these results. Four major themes were identified: (1) issues affecting the understanding of the tool; (2) issues that did not affect the understanding of the tool; (3) modifications to improve the tool; and (4) positive features of the tool. Advance QoL was well received, and feedback was positive. Adjustments were made according to young people’s comments and two self-reported versions are now available. It is essential to measure the key domains of QoL in advanced cancer. Advance QoL self-report versions will help target the specific needs of young people with this condition and their families. Full article

In the case of strong non-Gaussian noise in the measurement information of the distribution network, the strong non-Gaussian noise significantly interferes with the filtering accuracy of the state estimation model based on deep learning. To address this issue, this paper proposes an enhanced kernel ridge regression state estimation method based on ensemble empirical mode decomposition. Initially, ensemble empirical mode decomposition is employed to eliminate most of the noise data in the measurement information, ensuring the reliability of the data for subsequent filtering. Subsequently, the enhanced kernel ridge regression state estimation model is constructed to establish the mapping relationship between the measured data and the estimation residuals. By inputting the measured data, both estimation results and estimation residuals can be obtained. Finally, numerical simulations conducted on the standard IEEE-33 node system and a 78-node system in a specific city demonstrate that the proposed method exhibits high accuracy and robustness in the presence of strong non-Gaussian noise interference. Full article

Television plays a significant role in shaping the beliefs, perceptions, and attitudes of young individuals. Understanding the factors that influence these beliefs is essential for comprehending the impact of television programming on the youth population. This paper aims to investigate the correlations in beliefs regarding television programming among Greek youths aged 15–18 with their demographic characteristics, parental educational levels, school performance, and daily television viewing habits. To study the above, a structured questionnaire was electronically distributed to 204 Greek teenagers after obtaining parental consent. By identifying the factors associated with variations in beliefs, this study contributes to the existing literature on media influence and youth development. The findings indicate youth’s moderate levels of reflection regarding different aspects of television shows, including their presentation to the public and their influence on perceptions and attitudes. What is more, the youth audience haslow trust in TV news, and they moderately believe that the content of news is greatly influenced by governments and political elite agendas, while they consider TV as a major influential media type in shaping people’s political opinions. Notably, youngsters who considered themselves high-achieving students, as well as those who engaged in more frequent television viewing, including documentaries, news, and talk shows, exhibit increased concern and skepticism regarding the information presented in the television programs they watch. This is an important finding, because despite the low trust in TV as a medium, it continues to attract the attention of young people. Full article

This Research proposes an intelligent pruning method based on the improved Mask R-CNN (Mask Region-based Convolutional Neural Network) model to address the shortcomings of intelligent pruning technology for Sichuan pepper trees. Utilizing ResNeXt-50 as the backbone network, the algorithm optimizes the anchor boxes in the RPN (Region Proposal Network) layer to adapt to the complex morphology of pepper tree branches, thereby enhancing target detection and segmentation performance. Further reducing the quantization error of the RoI (Region of Interest) Align layer through bilinear interpolation, the algorithm innovatively introduces edge loss (L_edge) into the loss function to address the issue of blurred edge features caused by the overlap between retained and pruned branches. Experimental results demonstrate the outstanding performance of the improved Mask R-CNN model in segmenting and identifying pepper tree branches, achieving recognition accuracies of 92.2%, 96.3%, and 85.6% for Upright branches, Centripetal branches, and Competitive branches, respectively, while elevating the recognition accuracy of retained branches to 94.4%. Compared to the original Mask R-CNN, the enhanced model exhibits a 6.7% increase in the recognition rate of retained branches and a decrease of 0.12 in loss value, significantly enhancing recognition effectiveness. The research findings not only provide an effective tool for the precise pruning of pepper trees but also offer valuable insights for implementing intelligent pruning strategies for other fruit trees. Full article

This paper examines school leaders’ evasive attitudes towards race in Salt Lake City (SLC), Utah, between 1969 and 1975. Salt Lake’s unique demographic status as predominantly white and Mormon underscored elements of white anti-Black racism under the guise of innocence. Utilizing critical whiteness theory and historical inquiry to analyze archival documents and interviews, I highlight one white superintendent, Arthur Wiscombe, and his failed attempts to confront anti-Blackness in schools as he navigated his conflicting values of racial justice, good intentions, and white Niceness. Framing the past as prologue, I uncover the historical legacy of white supremacy’s influence on local school policies and leaders’ actions, and make explicit connections to the repetition of these patterns today. Contemporary iterations of white supremacy rely on the same tools of whiteness used during intense periods of integration and racial awareness in Salt Lake City in the 1960s and 1970s. I conclude that white educational leaders must look more closely at the ‘nice’, color-evasive discourse that enables them to maintain power and privilege in their communities. Full article

Background: Surgeons have limited knowledge of the lumbar intervertebral foramina. This study aimed to classify osteophytes in the lumbar intervertebral foramen and to determine their pathoanatomical characteristics, discuss their potential biomechanical effects, and contribute to developing surgical methods. Methods: We conducted a retrospective, non-randomized, single-center study involving 1224 patients. The gender, age, and anatomical location of the osteophytes in the lumbar intervertebral foramina of the patients were recorded. Results: Two hundred and forty-nine (20.34%) patients had one or more osteophytes in their lumbar 4 and 5 foramina. Of the 4896 foramina, 337 (6.88%) contained different types of osteophytes. Moreover, four anatomical types of osteophytes were found: mixed osteophytes in 181 (3.69%) foramina, osteophytes from the lower endplate of the superior vertebrae in 91 (1.85%) foramina, osteophytes from the junction of the pedicle and lamina of the upper vertebrae in 39 foramina (0.79%), and osteophytes from the upper endplate of the lower vertebrae in 26 (0.53%) foramina. The L4 foramen contained a significantly higher number of osteophytes than the L5 foramen. Osteophyte development increased significantly with age, with no difference between males and females. Conclusions: The findings show that osteophytic extrusions, which alter the natural anatomical structure of the lumbar intervertebral foramina, are common and can narrow the foramen. Full article

Background: Fabry disease is a progressive, X chromosome-linked lysosomal storage disorder with multiple organ dysfunction. Due to the absence or reduced activity of alpha-galactosidase A (AGAL), glycosphingolipids, primarily globotriaosyl-ceramide (Gb3), concentrate in cells. In heterozygous women, symptomatology is heterogenous and currently routinely used fluorometry-based assays measuring mean activity mostly fail to uncover AGAL dysfunction. The aim was the development of a flow cytometry assay to measure AGAL activity in individual cells. Methods: Conventional and multispectral imaging flow cytometry was used to detect AGAL activity. Specificity was validated using the GLA knockout (KO) Jurkat cell line and AGAL inhibitor 1-deoxygalactonojirimycin. The GLA KO cell line was generated via CRISPR-Cas9-based transfection, validated with exome sequencing, gene expression and substrate accumulation. Results: Flow cytometric detection of specific AGAL activity is feasible with fluorescently labelled Gb3. In the case of Jurkat cells, a substrate concentration of 2.83 nmol/mL and 6 h of incubation are required. Quenching of the aspecific exofacial binding of Gb3 with 20% trypan blue solution is necessary for the specific detection of lysosomal substrate accumulation. Conclusion: A flow cytometry-based assay was developed for the quantitative detection of AGAL activity at the single-cell level, which may contribute to the diagnosis of Fabry patients. Full article

The transition to more eco-friendly forms of transport is one of the main challenges for the Polish economy in the coming decades. Poland, as a member of the European Union, must adapt to the requirements regarding, in particular, reducing carbon dioxide emissions related to new vehicles. The implementation of these changes will require significant financial outlays and structural reconstruction of transport (both public and private), as well as remodeling of the functioning and habits of society. The aim of this study was to identify and analyze the challenges of the green transformation of transport in Poland. The following research methods were used: desk research, focus interview–expert research, and an original approach to the use of SWOT analysis. Based on the conducted analyses, Poland’s strategic position in the context of the “transport greening” process was determined. The strategy is in line with the concept of “reorganization”, calling for a thorough restructuring of the development strategy. This entails coordinated efforts, such as conducting in-depth evaluations of current strategies, securing increased funding, providing support for research, and implementing public education initiatives. In essence, the study emphasizes the necessity for significant endeavors to effectively manage the green transition of transportation in Poland. Full article

The Omicron variant of SARS-CoV-2, characterized by multiple subvariants including BA.1, XBB.1.5, EG.5, and JN.1, became the predominant strain in early 2022. Studies indicate that Omicron replicates less efficiently in lung tissue compared to the ancestral strain. However, the infectivity of Omicron in the gastrointestinal tract is not fully defined, despite the fact that 70% of COVID-19 patients experience digestive disease symptoms. Here, using primary human colonoids, we found that, regardless of individual variability, Omicron infects colon cells similarly or less effectively than the ancestral strain or the Delta variant. The variant induced limited type III interferon expression and showed no significant impact on epithelial integrity. Further experiments revealed inefficient cell-to-cell spread and spike protein cleavage in the Omicron spike protein, possibly contributing to its lower infectious particle levels. The findings highlight the variant-specific replication differences in human colonoids, providing insights into the enteric tropism of Omicron and its relevance to long COVID symptoms. Full article