Quantum cryptography revolutionizes secure information transfer, providing defense against both quantum and classical computational attacks. The primary challenge in extending the reach of quantum communication comes from the exponential decay of signals over long distances. We meet this challenge by experimentally realizing the Quantum-Protected Control-Based Key Distribution (QCKD) protocol, utilizing physical control over signal losses. By ensuring significant non-orthogonality of the leaked quantum states, this control severely constrains eavesdroppers’ capacities. We demonstrate the performance and scale of our protocol by experiments over a 1707 km long fiber line. The scalability of the QCKD opens the route for globally secure quantum-resistant communication. Full article

Over the last two decades, a considerable amount of equipment has been acquired (spreaders, seeders, sprayers, among others) to respond to the challenges of the precision agriculture (PA) concept. Most of this equipment has been purchased at a high cost. However, many of them, despite still being functional and equipped with sensors, actuators, and electronic processing units capable of adjusting to variations in speed, have become obsolete in terms of communication and incompatible with new monitoring and control systems based on the “Isobus” protocol. This work aims to present a solution for updating the control system (“Ferticontrol”) of a “Vicon RS-EDW” spreader with variable rate application (VRA), making it compatible with the “InCommand” system from “Ag Leader”. The solution includes serial protocol mediation using low-cost tools such as “Arduino” and “Raspberry Pi” microcontrollers and open-source software. The development shows that it is possible to implement a solution that is accessible to farmers in general. It also provides a niche business opportunity for young researchers to set up small technology-based enterprises associated with universities and research centers. These partnerships guarantee permanent innovation and represent a decisive step towards modern, technological, competitive, and sustainable agriculture. Full article

Given the various clinical manifestations that characterize Coronavirus Disease 2019 (COVID-19), the scientific community is constantly searching for biomarkers with prognostic value. Surfactant proteins A (SP-A) and D (SP-D) are collectins that play a crucial role in ensuring proper alveolar function and an alteration of their serum levels was reported in several pulmonary diseases characterized by Acute Respiratory Distress Syndrome (ARDS) and pulmonary fibrosis. Considering that such clinical manifestations can also occur during Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection, we wondered if these collectins could act as prognostic markers. In this regard, serum levels of SP-A and SP-D were measured by enzyme immunoassay in patients with SARS-CoV-2 infection (n = 51) at admission (T0) and after seven days (T1) and compared with healthy donors (n = 11). SP-D increased in COVID-19 patients compared to healthy controls during the early phases of infection, while a significant reduction was observed at T1. Stratifying SARS-CoV-2 patients according to disease severity, increased serum SP-D levels were observed in severe compared to mild patients. In light of these results, SP-D, but not SP-A, seems to be an eligible marker of COVID-19 pneumonia, and the early detection of SP-D serum levels could be crucial for preventive clinical management. Full article

A low-calcium microenvironment is imperative for spermatozoa maturation within the epididymis. Our previous work has shown that γ-glutamyl carboxylase (GGCX), the carboxylation enzyme of the matrix Gla protein (MGP), plays an essential role in epididymal calcium homeostasis and sperm maturation in rats and that the GGCX SNP mutation rs699664 was associated with asthenozoospermia (AZS) in humans. Here, we investigated the expression patterns of GGCX and MGP in the mouse epididymis and generated Ggcx^K325Q knock-in (KI) mice. We also tested the effects of this mutation on epididymal calcium homeostasis, sperm function, and male fertility in Ggcx^K325Q−/− mice. The results showed that both GGCX and MGP were enriched in all regions of the mouse epididymis, especially in the initial segment of the epididymis. Double immunofluorescence staining revealed that GGCX colocalized with MGP in the epithelial cells of the initial segment and caput regions as well as in the lumen of the corpus and cauda regions of the mouse epididymis. However, the Ggcx^K325Q−/− mice were fertile with normal epididymal morphology, sperm functions, and epididymal calcium concentration. Overall, our findings revealed that the Ggcx^K325Q mutation does not exert any discernible effect on male fertility in mice. Full article

The imbalance of gut microbiota is an important factor leading to inflammatory bowel disease (IBD). Diffusible signal factor (DSF) is a novel quorum-sensing signal that regulates bacterial growth, metabolism, pathogenicity, and host immune response. This study aimed to explore the therapeutic effect and underlying mechanisms of DSF in a zebrafish colitis model induced by sodium dextran sulfate (DSS). The results showed that intake of DSF can significantly improve intestinal symptoms in the zebrafish colitis model, including ameliorating the shortening of the intestine, reducing the increase in the goblet cell number, and restoring intestinal pathological damage. DSF inhibited the upregulation of inflammation-related genes and promoted the expression of claudin1 and occludin1 to protect the tightness of intestinal tissue. The gut microbiome analysis demonstrated that DSF treatment helped the gut microbiota of the zebrafish colitis model recover to normal at the phylum and genus levels, especially in terms of pathogenic bacteria; DSF treatment downregulated the relative abundance of Aeromonas hydrophila and Staphylococcus aureus, and it was confirmed in microbiological experiments that DSF could effectively inhibit the colonization and infection of these two pathogens in the intestine. This study suggests that DSF can alleviate colitis by inhibiting the proliferation of intestinal pathogens and inflammatory responses in the intestine. Therefore, DSF has the potential to become a dietary supplement that assists in the antibiotic and nutritional treatment of IBD. Full article

Plasmodium knowlesi is the only Plasmodium that causes zoonotic disease among the Plasmodium that cause infection in humans. It is fatal due to its short asexual growth cycle within 24 h. Lactate dehydrogenase (LDH), an enzyme that catalyzes the final step of glycolysis, is a biomarker for diagnosing infection by Plasmodium spp. parasite. Therefore, this study aimed to efficiently produce the soluble form of P. knowlesi LDH (PkLDH) using a bacterial expression system for studying malaria caused by P. knowlesi. Recombinant pET-21a(+)-PkLDH plasmid was constructed by inserting the PkLDH gene into a pET-21a(+) expression vector. Subsequently, the recombinant plasmid was inserted into the protein-expressing Escherichia coli Rosetta(DE3) strain, and the optimal conditions for overexpression of the PkLDH protein were established using this strain. We obtained a yield of 52.0 mg/L PkLDH from the Rosetta(DE3) strain and confirmed an activity of 483.9 U/mg through experiments. This methodology for high-efficiency PkLDH production can be utilized for the development of diagnostic methods and drug candidates for distinguishing malaria caused by P. knowlesi. Full article

In the last decades, historic European cities such as Lisbon have faced the challenge of aging dwellings, infrastructures, and a growing number of vacant buildings. These vacant spaces represent both a problem and an opportunity for the cities. While they detract from the city’s aesthetics and safety, they also offer a chance for renewal. Strategic reuse can address housing shortages, boost businesses, and revitalize neighborhoods. This study examines Lisbon’s efforts to revitalize vacant buildings from 2009 to 2022, with projections extending into the next five to ten years. Analyzing data on building use and reoccupation, the study reveals significant progress. A detailed survey of 1674 vacant buildings in 2009 shows many have been transformed. Then, through a GIS-based analysis, the immediate social and economic impacts of the requalification process are assessed, and two future development scenarios are evaluated. By 2022, 60% of these buildings (999) were reoccupied, expanding housing options and driving economic growth. Shops saw a 166% increase in occupancy, highlighting a thriving commercial sector. Additionally, 27% of the reoccupied buildings now hold tourism activities, including short-rental accommodation and hotels. Short- and long-term scenarios are proposed based on a comprehensive survey that captured the status, function, and preservation conditions of the vacant buildings within the city. These scenarios are planning tools for exploring potential future trajectories of urban development, allowing policymakers to anticipate issues, mitigate risks, and make informed decisions, shaping a more sustainable and resilient Lisbon. Full article

Many guidelines have been published to help diagnose food allergies, which have included feeding difficulties as a presenting symptom (particularly for non-IgE-mediated gastrointestinal allergies). This study aimed to investigate the prevalence of feeding difficulties in children with non-IgE-mediated gastrointestinal allergies and the association of such difficulties with symptoms and food elimination. An observational study was performed at Great Ormond Street Hospital for Children NHS Foundation Trust, London, UK. Children aged 4 weeks to 16 years without non-allergic co-morbidities who improved on an elimination diet using a previously published Likert scale symptom score were included. This study recruited 131 children, and 114 (87%) parents completed the questionnaire on feeding difficulties. Feeding difficulties were present in 61 (53.5%) of the 114 children. The most common feeding difficulties were regular meal refusals (26.9%), extended mealtimes (26.7%), and problems with gagging on textured foods (26.5%). Most children (40/61) had ≥2 reported feeding difficulties, and eight had ≥4. Children with feeding difficulties had higher rates of constipation and vomiting: 60.7% (37/61) vs. 35.8% (19/53), p = 0.008 and 63.9% (39/61) vs. 41.5% (22/53), p = 0.017, respectively. Logistic regression analysis demonstrated an association between having feeding difficulties, the age of the child, and the initial symptom score. Gender and the number of foods excluded in the elimination diet were not significantly associated with feeding difficulties. This study found that feeding difficulties are common in children with non-IgE-mediated gastrointestinal allergies, but there is a paucity of food allergy specific tools for establishing feeding difficulties, which requires further research in the long-term and consensus in the short term amongst healthcare professions as to which tool is the best for food allergic children. Full article

Locoregionally advanced and metastatic melanoma represent a challenging clinical problem, but in the era of immune checkpoint blockade and intralesional and infusional therapies, more options are available for use. Isolated limb infusion (ILI) was first introduced in the 1990s for the management of advanced melanoma, followed by the utilization of isolated extremity perfusion (ILP). Following this, intralesional oncolytic viruses, xanthene dyes, and cytokines were introduced for the management of in-transit metastases as well as unresectable, advanced melanoma. In 2015, the Food and Drug Administration (FDA) approved the first oncolytic intralesional therapy, talimogene laherparepvec (T-VEC), for the treatment of advanced melanoma. Additionally, immune checkpoint inhibition has demonstrated efficacy in the management of advanced melanomas, and this improvement in outcomes has been extrapolated to aid in the management of in-transit metastatic disease. Finally, percutaneous hepatic perfusion (PHP), also approved by the FDA, has been reported to have a significant impact on the treatment of hepatic disease in uveal melanoma. While some of these treatments have less utility due to inferior outcomes as well as higher toxicity profiles, there are selective patient profiles for which these therapies carry a role. This review highlights intralesional and infusional therapies for the management of metastatic melanoma. Full article

Policies for reducing the environmental impact of light-duty vehicles are restarting in Brazil. While electrification attracts more engagement than other forms of carbon footprint reduction, the use of ethanol could achieve faster gains with the existing infrastructure. The prominence of entry-level cars has reduced, which may be due to several factors, making these vehicles uneconomical for manufacturers to produce. This situation is different from those in other countries. It is our premise that other modes, such as walking, cycling, and public transportation, are the main methods of decarbonization. However, most need improvements in terms of security and attractiveness for consumers. Hence, the internal combustion engine will dominate the Brazilian fleet for quite some time. This article discusses the policies used to incentivize the implementation of entry-level vehicles in contrast with electric vehicles based on regression models of the Brazilian fleet. By providing a history of the 1.0 L engine in Brazil, this article debates replacing the indirect displacement taxation with direct CO₂ taxation, which can make the Brazilian entry-level car practical again, with the possibility of adopting some incentives. This logic will cause a fleet renovation and more rational energy use. The distinguishing feature of this article is the proposition of possible public policies based on a regression model of the national fleet. The key findings show that the fleet is getting older and heavier, consuming more energy, and emitting greater amounts of CO₂ per unit of displacement, for example, 1.0 L. Furthermore, the carbon footprint of the electricity mix is similar to that of the produced ethanol (from well to wheel, the carbon footprint is the same). This highlights the necessity for national policy adjustments and effective public policies to reduce carbon emissions from the energy sector for small vehicles. Full article

The treatment of canine leishmaniosis commonly involves meglumine antimoniate with allopurinol or miltefosine with allopurinol. This study aims to compare the clinical and clinicopathological efficacy at 28–30 days of conventional dosing regimens for both treatments using the critically appraised topic methodology. A comprehensive search across three databases (PubMed, CAB Abstracts, and Web of Science) from March 2004 to September 2023 yielded 16 relevant articles, encompassing 325 ogs treated with meglumine antimoniate and allopurinol, and 273 dogs treated with miltefosine and allopurinol. The findings indicated a significantly higher rate of complete clinical cure in the group treated with meglumine antimoniate and allopurinol. Most dogs in both groups exhibited improvement in clinicopathological alterations after one month of treatment. No significant difference was observed in the number of dogs that showed a negative Leishmania qPCR between the two groups, one month post-treatment. However, quantitative serology results were not commonly reported in the available data and therefore this aspect could not be compared. Full article

Additive manufacturing workflows generate large amounts of data in each phase, which can be very useful for monitoring process performance and predicting the quality of the finished part if used correctly. In this paper, a framework is presented that utilises machine learning methods to predict porosity defects in printed parts. Data from process settings, in-process sensor readings, and post-process computed tomography scans are first aligned and discretised using a voxelisation approach to create a training dataset. A multi-step classification system is then proposed to classify the presence and type of porosity in a voxel, which can then be utilised to find the distribution of porosity within the build volume. Titanium parts were printed using a laser powder bed fusion system. Two discretisation techniques based on voxelisation were utilised: a defect-centric and a uniform discretisation method. Different machine learning models, feature sets, and other parameters were also tested. Promising results were achieved in identifying porous voxels; however, the accuracy of the classification requires improvement before being applied industrially. The potential of the voxelisation-based framework for this application and its ability to incorporate data from different stages of the additive manufacturing workflow as well as different machine learning models was clearly demonstrated. Full article

Rotavirus infection continues to be a significant public health problem in developing countries, despite the availability of several vaccines. The efficacy of oral rotavirus vaccines in young children may be affected by significant immunological differences between individuals in early life and adults. Therefore, understanding the dynamics of early-life systemic and mucosal immune responses and the factors that affect them is essential to improve the current rotavirus vaccines and develop the next generation of mucosal vaccines. This review focuses on the advances in T-cell development during early life in mice and humans, discussing how immune homeostasis and response to pathogens is established in this period compared to adults. Finally, the review explores how this knowledge of early-life T-cell immunity could be utilized to enhance current and novel rotavirus vaccines. Full article

Graphene oxide (GO) was used as an ion-to-electron transducer for all-solid-state nitrate electrodes based on an alkyl ammonium salt as the sensing element. Commercially available carbon screen-printed electrodes modified with GO were used as conductive substrates, whose morphology and distribution along the surface were evaluated by scanning electron microscopy and Raman spectroscopy. The potentiometric performance of the GO-based electrodes revealed a Nernstian slope of −53.5 ± 2.0 mV decade⁻¹ (R² = 0.9976 ± 0.0015) in the range from 3.0 × 10⁻⁶ to 10⁻² M and a lower limit of detection of 1.9 × 10⁻⁶ M. An impressive reproducibility between equally prepared electrodes (n = 15) was demonstrated by a variation of <6% for the calibration parameters. Constant current chronopotentiometry and water layer tests were used to evaluate the potential signal stability, providing similar performance to previously published works with graphene-based ion-selective electrodes. Notably, the GO-based sensors showed the absence of a water layer, a long-term drift of 0.3 mV h⁻¹, and a stable performance (LOD and sensitivity) over 3 months. The applicability of the proposed sensors was demonstrated in determining nitrate levels in water samples with great accuracy, yielding recovery values from 87.8 to 107.9%, and comparable (p > 0.05) results to a commercial nitrate probe. These findings demonstrate the use of GO as an alternative ion-to-electron transducer for the fabrication of all-solid-state potentiometric electrodes. Full article

Currently, one of the most important problems of environmental protection is the deep and complex processing of mineral raw materials. This problem is especially relevant when processing substandard ores and production waste, one of which is phosphogypsum. This study examines the process of CaSO₄/CaS composite material formation during the reduction of phosphogypsum with citric acid. The composite structure formation mechanism is proposed. The resulting materials are characterized using various methods, including X-ray diffraction (XRD), transmission electron microscopy, the Scherrer method, thermogravimetric analysis (TGA), and FT-IR spectroscopy. The reduced sample emits orange radiation in the range of 500–750 nm with a quantum yield of 0.17. Experimental results showed that the sample decomposition process in the solid state consisted of two components with a predominant contribution from the long-lived component (~46 ns). The optimal conditions for producing luminescent materials by reducing phosphogypsum with citric acid were determined: a heat treatment temperature of 1073 K, a holding time of 60 min, and a reducing agent mole fraction of 37%. It was found that an increase in temperature with a simultaneous decrease in heat treatment time, as well as a decrease in temperature with a simultaneous increase in heat treatment time, led to a decrease in the luminescent properties of the synthesized material compared to optimal values. The results can be used to develop technology for recycling large-tonnage waste from the chemical industry into luminescent materials. Full article

The dengue virus, the primary cause of dengue fever, dengue hemorrhagic fever, and dengue shock syndrome, is the most widespread mosquito-borne virus worldwide. In recent decades, the prevalence of dengue fever has increased markedly, presenting substantial public health challenges. Consequently, the development of an efficacious vaccine against dengue remains a critical goal for mitigating its spread. Our research utilized Celcradle™, an innovative tidal bioreactor optimized for high-density cell cultures, to grow Vero cells for dengue virus production. By maintaining optimal pH levels (7.0 to 7.4) and glucose concentrations (1.5 g/L to 3.5 g/L) during the proliferation of cells and viruses, we achieved a peak Vero cell count of approximately 2.46 × 10⁹, nearly ten times the initial count. The use of Celcradle™ substantially decreased the time required for cell yield and virus production compared to conventional Petri dish methods. Moreover, our evaluation of the immunogenicity of the Celcradle™-produced inactivated DENV4 through immunization of mice revealed that sera from these mice demonstrated cross-reactivity with DENV4 cultured in Petri dishes and showed elevated antibody titers compared to those from mice immunized with virus from Petri dishes. These results indicate that the dengue virus cultivated using the Celcradle™ system exhibited enhanced immunogenicity relative to that produced in traditional methods. In conclusion, our study highlights the potential of the Celcradle™ bioreactor for large-scale production of inactivated dengue virus vaccines, offering significant promise for reducing the global impact of dengue virus infections and accelerating the development of effective vaccination strategies. Full article

In the context of COVID-19, the efforts undertaken for epidemic control have imposed limitations on the multifaceted development of China. This manuscript utilizes Baidu migration data from 2019 to 2023 to classify the current developmental status of urban agglomerations (UAs) in China. The explication of network structure is achieved through the computation of metrics that capture network structural connectivity and hierarchical attributes. Additionally, an inquiry into the spatio-temporal differentiation of the UAs’ network structure is carried out, encompassing three phases: before COVID-19, the normalization stage of COVID-19, and after COVID-19. Furthermore, Quantitative Analysis of Patterns (QAP) is employed to assess the impact of diverse influencing factors. The analysis yields several key findings: ① The impact of COVID-19 on the network structure of China’s UAs manifests in two discernible stages—initial impact disruption and subsequent recovery and reconstruction. ② The exploration of pertinent influencing factors during the primary stage of UA development is impeded. ③ The growth stage and the UAs with a high level of development exhibit have a closely intertwined relationship, fostering a more rational hierarchical structure and demonstrating an enhanced capacity for swift recovery. ④ It is discerned that economic development level, medical facility standards, transportation infrastructure capacity, spatial proximity, and innovation accessibility exert a discernible influence on the network structure of UAs. Importantly, the extent of impact varies across different periods and types of UAs. Full article

CO₂ foam fracturing in tight and shale reservoirs is a revolutionary technique for commercially viable production. Nevertheless, the screening of foaming agents used in CO₂ foam fracturing fluid and the understanding of foaming mechanisms have not been sufficiently investigated. This study aimed to provide a comprehensive method for evaluating and selecting an optimized foaming agent for CO₂ foam fracturing fluid integrating macroscopic and microscopic approaches through laboratory experiments and molecular dynamics simulations. The relationship between the molecular structure of the foaming agent and its corresponding foaming effect was elucidated by taking the interaction between CO₂ and the foaming agent into account. Foam evaluation experiments indicated that the anionic surfactants exhibited superior foaming capacity and inferior stability compared to zwitterionic and non-ionic surfactants. The molecular dynamics simulation results demonstrated that the foaming mechanism of the CO₂ foaming agent relied on the equilibriums between CO₂-surfactant, CO₂-water, and surfactant–water interactions. At the same time, it was found that if the molecular structure of the surfactant contained functional groups that could produce hydrogen bonding with CO₂, the stability of the foaming effect improved to a certain extent, but the foaming volume was not obvious. The classic hydrophilic–lipophilic balance (HLB) theory was not applicable when screening the CO₂ foaming agents. It was found that the ionic surfactants with CO₂-philic groups and linear structures were suitable as the main foaming agents for CO₂ foam fracturing fluids, while non-ionic surfactants with significant steric hindrance were suitable as auxiliary foaming agents. This study provides valuable guidance for selecting cost-effective foaming agents on-site and adds to the understanding of the relationship between the molecular structure of foaming agents and their foaming effects. Full article

Onychomycoses are nail infections that require prolonged therapy and have high recurrence rates. Dermatophytes are the main etiological agents of these infections, followed by yeasts and non-dermatophyte filamentous fungi. The limited antifungal arsenal used to treat onychomycosis and the change in the susceptibility profile of these agents contribute to the chronicity and recalcitrant profile of infections. The present study aimed to determine the antifungal activity of extracts obtained from pure and mixed cultures of Candida parapsilosis, Trichophyton mentagrophytes, and Trichophyton rubrum. Additionally, in vivo toxicity tests with Galleria mellonella and time-kill assays were carried out. The susceptibility profiles of dermatophytes were determined using a microdilution technique with minimum inhibitory concentrations (MICs) between 250 and 8000 µg/mL. The time-kill assay, compared to growth control, resulted in the death of dermatophytes within 48 h. No toxicity of the extracts was detected in experiments with Galleria mellonella larvae under the test conditions. The extracts of pure and mixed cultures of Candida parapsilosis and dermatophytes present antifungal activity against T. mentagrophytes and T. rubrum. Isolating and identifying compounds in the extracts may allow the development of new therapeutic approaches to control fungal infections. Full article

Traditional exhaust-gas turbocharging exhibits hysteresis under variable working conditions. To achieve rapid-intake supercharging, this study investigates the synergistic coupling process between the detonation and diesel cycles using gasoline as fuel. A numerical simulation model is constructed to analyze the detonation characteristics of a pulse-detonation combustor (PDC), followed by experimental verification. The comprehensive process of the flame’s deflagration-to-detonation transition (DDT) and the formation of the detonation wave are discussed in detail. The airflow velocity, DDT time, and peak pressure of detonation tubes with five different blockage ratios (BR) are analyzed, with the results imported into a one-dimensional GT-POWER engine model. The results indicate that the generation of detonation waves is influenced by flame and compression wave interactions. Increasing the airflow does not shorten the DDT time, whereas increasing the BR causes the DDT time to decrease and then increase. Large BRs affect the initiation speed of detonation in the tube, while small BRs impact the DDT distance and peak pressure. Upon connection to the PDC, the transient response rate of the engine is slightly improved. These results can provide useful guidance for improving the transient response characteristics of engines. Full article

Cerebrospinal fluid (CSF) is a body fluid that can be used for the diagnosis of various diseases. However, CSF collection requires an invasive and painful procedure called a lumbar puncture (LP). This procedure is applied to any patient with a known risk of central nervous system (CNS) damage or neurodegenerative disease, regardless of their age range. Hence, this can be a very painful procedure, especially in infants and elderly patients. On the other hand, the detection of disease biomarkers in CSF makes diagnoses as accurate as possible. This review aims to explore novel electrochemical biosensing platforms that have impacted biomedical science. Biosensors have emerged as techniques to accelerate the detection of known biomarkers in body fluids such as CSF. Biosensors can be designed and modified in various ways and shapes according to their ultimate applications to detect and quantify biomarkers of interest. This process can also significantly influence the detection and diagnosis of CSF. Hence, it is important to understand the role of this technology in the rapidly progressing field of biomedical science. Full article

Background and Objectives: Despite the established role of subtalar joint arthrodesis (SJA) for treatment of subtalar osteoarthritis, achieving bone union remains challenging, with up to 46% non-union rates. Adequate compression and stable fixation are crucial for successful outcomes, with internal screw fixation being the gold standard for SJA. The delta configuration, featuring highly divergent screws, offers stability, however, it can result in hardware irritation in 20–30% of patients. Solutions to solve this complication include cannulated compression screw (CCS) countersinking or cannulated compression headless screw (CCHS) application. The aim of this biomechanical study was to investigate the stability of a delta configuration for SJA utilizing either a combination of a posterior CCHS and an anterior CCS or a standard two-CCS combination. Materials and Methods: Twelve paired human cadaveric lower legs were assigned pairwise to two groups for SJA using either two CCSs (Group 1) or one posterior CCHS and one anterior CCS (Group 2). All specimens were tested under progressively increasing cyclic loading to failure, with monitoring of the talocalcaneal movements via motion tracking. Results: Initial stiffness did not differ significantly between the groups, p = 0.949. Talocalcaneal movements in terms of varus–valgus deformation and internal–external rotation were significantly bigger in Group 1 versus Group 2, p ≤ 0.026. Number of cycles until reaching 5° varus–valgus deformation was significantly higher in Group 2 versus Group 1, p = 0.029. Conclusions: A delta-configuration SJA utilizing a posterior CCHS and an anterior CCS is biomechanically superior versus a standard configuration with two CCSs. Clinically, the use of a posterior CCHS could prevent protrusion of the hardware in the heel, while an anterior CCS could facilitate less surgical time and thus less complication rates. Full article