Abstract
Milled wood lignin (MWL) of Eucalyptus globulus has been treated by a selective degradation method for β-O-4 linkages, which lets the lignin carbohydrate complexes (LCC) intact. The method consists of four reaction steps: (1) γ-tosylation, (2) thioetherification, (3) sulfonylation and (4) mild alkali treatment (γ-TTSA method). Each step was followed by spectroscopies for chemical structural analysis; especially, the HSQC-NMR analysis was in focus. It was demonstrated that β-O-4 linkages were selectively and quantitatively cleaved by the γ-TTSA method while the β-β linkages in the MWL remained intact. The method leads to an enrichment of LCCs.
We thank Dr Kazutsune Tsurumi of Oji Paper Co., Ltd (Tokyo, Japan) for providing the wood sample of E. globulus.
References
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