Activity staining of cellulases in polyacrylamide gels containing mixed linkage β-glucans
References (16)
Anal. Biochem
(1983)- et al.
J. Biol. Chem
(1980) - et al.
J. Biol. Chem
(1951) - et al.
FEMS Microbiol. Lett
(1985) - et al.
Gene
(1983) - et al.
Ann. Microbiol. (Paris)
(1985) - et al.
FEBS Lett
(1982) - et al.
Biochemie
(1986)
Cited by (158)
Expression and functional characterization in yeast of an endoglucanase from Bacillus sonorensis BD92 and its impact as feed additive in commercial broilers
2021, International Journal of Biological MacromoleculesExogenous xylanase expression simultaneously with the indigenous cellulase to increase the cellulose hydrolysis efficiency
2019, International Biodeterioration and BiodegradationCitation Excerpt :Endoglucanase zymogram was performed according to the processes suggested by Lee (Lee et al., 2014). Zymogram analysis of β-glucosidase was conducted following the method as described by Schwarz (Schwarz et al., 1987). Crude cellulase powder and steam-exploded wheat straw was prepared as described by Xue (Xue et al., 2017a,b).
Production of thermostable β-glucosidase and CMCase by Penicillium sp. LMI01 isolated from the Amazon region
2018, Electronic Journal of BiotechnologyCitation Excerpt :After electrophoresis, the SDS-PAGE gel was stained with Coomassie Brilliant Blue R-250 and then destained overnight using a distaining solution containing 50% methanol and 12% acetic acid for visualization of the protein bands. The zymography gel was incubated in 1% Triton X-100 buffer (v/v) under gentle stirring for 1 h to remove the SDS, washed three times with distilled water, incubated in reaction buffer (50 mM sodium citrate, pH 4.8) for 2 h, stained with Congo red (0.1%) under agitation for 20 min, and successively washed with 1-M NaCl solution until the bands were visible [41,42,43]. Data were analyzed using BioEstat® version 5.3 software (Mamirauá Sustainable Development Institute, Amazonas, Brazil — http://www.mamiraua.org.br) and plotted using the ORIGIN© version 2016 tool.
A new earthworm cellulase and its possible role in the innate immunity
2017, Developmental and Comparative Immunology