Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels

doi:10.1006/abio.1993.1308

Analytical Biochemistry

Volume 212, Issue 1, July 1993, Pages 168-172

https://doi.org/10.1006/abio.1993.1308 Get rights and content

Abstract

A very simple method is described which allows the separation of DNA molecules in a wide molecular weight range (from 0.6 to about 30 kb) in the same electrophoresis agarose gel. This is based on the achievement of a voltage gradient through a simple device consisting of a Plexiglas plate placed slantwise with respect to the gel surface plane, submerged in the electrophoretic running buffer. Further applications of our system are also described.

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Regular ArticleVoltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels

Abstract

Regular Article
Voltage Gradient Electrophoresis of Nucleic Acids on Agarose Gels