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Extracting and Physicochemical Properties of Carotenoprotein from Shrimp Processing Waste by Proteases-Mediated Hydrolysis

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Abstract

In this study, carotenoprotein from Pacific white shrimp (Litopenaeus vannamei) processing waste was extracted with the aid of alcalase (CP-A) and pepsin (CP-P) at various levels (0–4 units/100 g waste) for different times (0–240 min). Recovery of carotenoids and protein increased with 3 unit/g of enzyme and hydrolysis times until 180 min (p < 0.05). The extracted carotenoprotein by pepsin and alcalase consisted of 72.11–75.32% protein and carotenoids content was in the range of 330–530 µg/g samples. The phenylalanine, lysine, methionine, and valine as essential amino acids were higher at CP-A and CP-P. The dominant non-essential amino acids in carotenoproteins were aspartic acid, glutamic acid, glycine, and alanine. It was rich in mono and polyunsaturated fatty acids. The CP-A showed higher content of docosahexaenoic acid and eicosapentaenoic acid (8.52 and 6.49%) than CP-P (5.55 and 5.49%). The saturated fatty acids were reduced after enzymatic hydrolysis and contents were higher in carotenoproteins. The extracted samples showed a significant amount of mineral contents. Sodium, phosphorus, magnesium, and potassium contents were found higher in CP-A. The lead and copper were reduced as a result of hydrolysis. Therefore, carotenoprotein from processing residue of pacific white shrimp could be used as the value-added nutritious enriching food or feed powder.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

Abbreviations

CPs:

Carotenoproteins

CP-A:

Carotenoprotein Recovered by Alcalase

CP-P:

Carotenoprotein Recovered by Pepsin

SW:

Shrimp Waste

SFA:

Saturated Fatty Acids

MUFA:

Mono Unsaturated Fatty Acids

PUFA:

Poly Unsaturated Fatty Acids

BSA:

Bovine Serum Albumin

BF3:

Boron triFluoride

FAMEs:

Fatty Acid Methyl Esters

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Authors and Affiliations

  1. Department of Seafood Processing, Faculty of Fisheries and Environmental Science, Gorgan University of Agriculture Sciences and Natural Resources, Gorgan, Iran

    Parastoo Pourashouri & Hojat Mirsadeghi

  2. Department of Fisheries, Faculty of Marine Natural Resources, Khorramshahr University of Marine Science and Technology, Khorramshahr, Iran

    Aynaz Khodanazary

Authors
  1. Parastoo Pourashouri
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  2. Hojat Mirsadeghi
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  3. Aynaz Khodanazary
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Contributions

PP: supervision, conceptualization, formal analysis, writing—reviewing and editing; HM: methodology, data curation, resources; AK: visualization, investigation.

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Correspondence to Parastoo Pourashouri.

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Pourashouri, P., Mirsadeghi, H. & Khodanazary, A. Extracting and Physicochemical Properties of Carotenoprotein from Shrimp Processing Waste by Proteases-Mediated Hydrolysis. Waste Biomass Valor 13, 1169–1178 (2022). https://doi.org/10.1007/s12649-021-01561-4

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