Abstract. Currently, the Heidelberg Basin is under investigation by new cored research boreholes to enhance the understanding concerning the control on Pliocene and Quaternary sedimentation by (neo)tectonics and climate. The Heidelberg Basin is expected to serve as a key location for an improved correlation of parameters that characterise the climate evolution in North Europe and the Alpine region. The recovery of sediment successions of high temporal resolution that are complete with respect to the deposition of Pleistocene glacials and interglacials in superposition is of special importance. Prior to the new research boreholes in Viernheim and Heidelberg geophysical pre-site surveys were performed to identify borehole locations that best achieve these requirements. In the area of the Heidelberg Basin the strongest negative gravity anomaly of the entire Upper Rhine Graben is observed (apart from the Alps), hinting at anomalously thick sediment deposits. However, especially reflection seismic profiles contributed significantly to the decision about the borehole locations. In the city of Heidelberg for the first time, the depocentre of the Heidelberg Basin, as indicated by additional subsidence compared to its surroundings, was mapped. In this area, sediments dip towards the eastern margin of the Upper Rhine Graben. This is interpreted to represent a rollover structure related to the maximum subsidence of the Upper Rhine Graben in this region. At the Viernheim borehole location the seismic survey revealed several faults. Although these faults are mainly restricted to depths greater than 225 m, the borehole location was fi nally adjusted with respect to this information.