Abstract
TREATMENT of murine T lymphocytes with concanavalin A (con A) at concentrations as low as 10−17 g ml−1 produces significant increases in their electrophoretic mobility. The effect is blocked by α-methyl-D-mannopyranoside (α-MM). Phytohaemagglutinin (PHA) produces similar changes in T-cell mobility while treatment with lipopolysaccharide (LPS), a B-lymphocyte mitogen, does not. We present here evidence indicating that the altered mobility of con A-treated T cells is due to the rapid release of a factor from the few cells reacting directly with the lectin.
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BLUME, P., MALLEY, A., KNOX, R. et al. Electrophoretic mobility as a sensitive probe of lectin–lymphocyte interaction. Nature 271, 378–380 (1978). https://doi.org/10.1038/271378a0
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DOI: https://doi.org/10.1038/271378a0
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