Abstract
We have previously described several complementary DNA clones isolated because they correspond to messenger RNAs present at higher levels in the simian virus 40 (SV40)-transformed BALB/c 3T3 cell line SV3T3 Cl38 than in the normal, parental BALB/c 3T3 line1. One of these clones, pAG64, hybridizes to RNAs which, while present in BALB/c 3T3 cells, are 10–20-fold more abundant in SV3T3 Cl38 and are found at high levels in a wide variety of transformed cell lines1,2. Nucleotide sequence analysis showed that pAG64 encodes a class I antigen of the major histocompatibility complex2. To ascertain the identity of pAG64, we compared its sequence with the available sequences of d haplotype class I antigen genes [K locus3, L locus4, D locus5 and the Qa gene defined by genomic clone 27.1 (ref. 6)] and found that it showed multiple clustered differences from each of these sequences2. We therefore concluded that it was not derived from the H–2Kd, H–2Ld or H–2Dd genes and thus must correspond to one of the other class I antigen genes, namely those of the Qa/Tla complex2, although it was clearly not the Qa gene defined by the genomic clone 27.1. We now report subsequent findings which indicate that pAG64 in fact corresponds to the H–2Dd gene and not to a Qa/Tla gene.
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Brickell, P., Latchman, D., Murphy, D. et al. The class I major histocompatibility antigen gene activated in a line of SV40-transformed mouse cells is H–2Dd, not Qa/Tla. Nature 316, 162–163 (1985). https://doi.org/10.1038/316162a0
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DOI: https://doi.org/10.1038/316162a0
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