Abstract
A 5.1 kilobase-pair segment of rabbit chromosomal β-globin DNA was joined to pJDB219, a plasmid consisting of pMB9, the 2-µ, yeast plasmid and the yeast leu-2 gene. Saccharomyces cerevisiae transformed with the globin DNA-containing hybrid produced β-globin-specific RNA. As compared to mature β-globin mRNA, these transcripts lacked 20–40 nucleotides from the 5′ end, contained all of the small intron and extended to about the middle of the large intron. Thus, no splicing of the primary β-globin transcript could be detected in the yeast cells.
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Beggs, J., van den Berg, J., van Ooyen, A. et al. Abnormal expression of chromosomal rabbit β-globin gene in Saccharomyces cerevisiae. Nature 283, 835–840 (1980). https://doi.org/10.1038/283835a0
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DOI: https://doi.org/10.1038/283835a0
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