Tropical cyclones (TCs) are characterized by robust vortical motion and intense thermodynamic processes, often causing damage in coastal cities as they result in landfall. Accurately estimating the ensemble mean of TC precipitation is critical for forecasting and remains a foremost global challenge. In this study, we develop an ensemble algorithm based on the feature-oriented mean (FM) suitable for spatially discrete variables in precipitation ensembles. This method can adjust the locations of ensemble precipitation fields to reduce the location-related deviations among ensemble members, ultimately enhancing the ensemble mean forecast skill for TC precipitation. To evaluate the feasibility of the FM in TC precipitation ensemble forecasting, 18 landing TC cases in China from 2019 to 2021 were selected for validation. For precipitation forecasts of the landing TCs with a varying leading time, we conducted a comprehensive quantitative evaluation and comparison of the precipitation forecast skills of the FM and arithmetic mean (AM) algorithms. The results indicate that the field adjustment algorithm in the FM can effectively align with the TC precipitation structure and the location of the ensemble mean, reducing the spatial divergence among precipitation fields. The FM method demonstrates superior performance in the equitable threat score, probability of detection, and false alarm ratio compared with the AM, exhibiting an overall improvement of around 10%. Furthermore, the FM ensemble mean shows a higher pattern of the correlation coefficient with observations and has a smaller root mean square error than the AM ensemble mean, signifying that the FM method can better preserve the characteristics of the precipitation structure. Additionally, an object-based diagnostic evaluation method was used to verify forecast results, and the results suggest that the attribute distribution of FM forecast objects more closely resembles that of observed precipitation objects (including the area, longitudinal and latitudinal centroid locations, axis angle, and aspect ratio). Full article

Obtaining accurate basic parameters for long hole blasting is challenging, and the resulting vibration damage significantly impacts key surface facilities. Predicting ground vibration velocity accurately and mitigating the harmful effects of blasting are crucial aspects of controlled blasting technology. This study focuses on the prediction of ground vibration velocity induced by underground long hole blasting tests. Utilizing the fitting equation based on the US Bureau of Mines (USBM) formula as a baseline for predicting peak particle velocity, two machine learning models suitable for small sample data, Support Vector Regression (SVR) machine and Random Forest (RF), were employed. The models were optimized using the particle swarm optimization algorithm (PSO) to predict peak particle velocity with multiple parameters specific to long hole blasting. Mean absolute error (MAE), mean Squared error (MSE), and coefficient of determination (R²) were used to assess the model predictions. Compared with the fitting equation based on the USBM model, both the Support Vector Regression (SVR) and Random Forest (RF) models accurately and effectively predict peak particle velocity, enhancing prediction accuracy and efficiency. The SVR model exhibited slightly superior predictive performance compared to the RF model. Full article

Recent years have witnessed unprecedented progress in therapeutic gene editing, revolutionizing the approach to treating genetic disorders. In this comprehensive review, we discuss the progression of milestones leading to the emergence of the clustered regularly interspaced short palindromic repeats (CRISPR)-based technology as a powerful tool for precise and targeted modifications of the human genome. CRISPR-Cas9 nuclease, base editing, and prime editing have taken center stage, demonstrating remarkable precision and efficacy in targeted ex vivo and in vivo genomic modifications. Enhanced delivery systems, including viral vectors and nanoparticles, have further improved the efficiency and safety of therapeutic gene editing, advancing their clinical translatability. The exploration of CRISPR-Cas systems beyond the commonly used Cas9, such as the development of Cas12 and Cas13 variants, has expanded the repertoire of gene editing tools, enabling more intricate modifications and therapeutic interventions. Outstandingly, prime editing represents a significant leap forward, given its unparalleled versatility and minimization of off-target effects. These innovations have paved the way for therapeutic gene editing in a multitude of previously incurable genetic disorders, ranging from monogenic diseases to complex polygenic conditions. This review highlights the latest innovative studies in the field, emphasizing breakthrough technologies in preclinical and clinical trials, and their applications in the realm of precision medicine. However, challenges such as off-target effects and ethical considerations remain, necessitating continued research to refine safety profiles and ethical frameworks. Full article

By leveraging a multiscale geographically weighted regression (MGWR) model, this paper delves into the intricate factors that influence housing prices in the prototypical mountainous cityscape of Chongqing’s central urban area. The key findings are as follows: Firstly, the distribution of housing prices in the study region exhibits pronounced spatial heterogeneity, with the core area exhibiting a distinct “high-high” clustering pattern and manifesting characteristics of a multicenter group distribution. Secondly, the MGWR model effectively assigns an individual bandwidth to each feature quantity, allowing for a more nuanced portrayal of the varying influence scales exerted by diverse variables. Lastly, the study reveals that factors such as property cost, greening rate, building age, and proximity to rivers have a notable negative impact on housing prices, whereas, educational facilities exert a marked positive influence. Elevation, floor area ratio, and distance from the Central Business District (CBD) exhibit a more complex influence on housing prices. Full article

The role of critical infrastructures in maintaining the functioning of the economy and society and ensuring national security, particularly their durability in delivering essential services during crises, including natural disasters such as earthquakes, is critical. This work introduces an analytical methodology to quantify potential earthquake damage to power stations and evaluate the cost-effectiveness of measures to enhance their seismic resistance. By employing fragility curves and probabilistic risk analyses, this approach provides a structured framework for the comprehensive assessment of risks and the identification of economically practical mitigation strategies. A detailed examination of strategies to protect critical power station components against seismic activity is presented, revealing that a minor investment relative to the overall project budget for earthquake-proofing measures is economically effective. This investment, representing a marginal fraction of 0.5% of the total project expenditure significantly reduces the seismic risk of power station failure by 36%. Reinforcing essential elements, including switching stations, water treatment facilities, and water tanks, is emphasized to ensure their continued operation during and after an earthquake. This research highlights the critical significance of integrating risk assessment with benefit-to-cost analysis in strategic decision-making processes, supporting the prioritization of investments in infrastructure enhancements. These enhancements promise substantial reductions of risks at minimal costs, thus protecting essential services against the impacts of natural disasters. This research contributes to state-of-the-art research in critical infrastructures resilience. Full article

Post-traumatic stress disorder (PTSD) is a debilitating psychological condition that may develop in certain individuals following exposure to life-threatening or traumatic events. Distressing symptoms, including flashbacks, are characterized by disrupted stress responses, fear, anxiety, avoidance tendencies, and disturbances in sleep patterns. The enduring effects of PTSD can profoundly impact personal and familial relationships, as well as social, medical, and financial stability. The prevalence of PTSD varies among different populations and is influenced by the nature of the traumatic event. Recently, zebrafish have emerged as a valuable model organism in studying various conditions and disorders. Zebrafish display robust behavioral patterns that can be effectively quantified using advanced video-tracking tools. Due to their relatively simple nervous system compared to humans, zebrafish are particularly well suited for behavioral investigations. These unique characteristics make zebrafish an appealing model for exploring the underlying molecular and genetic mechanisms that govern behavior, thus offering a powerful comparative platform for gaining deeper insights into PTSD. This review article aims to provide updates on the pathophysiology of PTSD and the genetic responses associated with psychological stress. Additionally, it highlights the significance of zebrafish behavior as a valuable tool for comprehending PTSD better. By leveraging zebrafish as a model organism, researchers can potentially uncover novel therapeutic interventions for the treatment of PTSD and contribute to a more comprehensive understanding of this complex condition. Full article

Anthracnose, caused by the fungus Colletotrichum lindemuthianum, poses a significant and widespread threat to the common bean crop. The use of plant genetic resistance has proven to be the most effective strategy for managing anthracnose disease. The Amendoim Cavalo (AC) Andean cultivar has resistance against multiple races of C. lindemuthianum, which is conferred by the Co-AC gene. Fine mapping of this resistance gene to common bean chromosome Pv01 enabled the identification of Phvul.001G244300, Phvul.001G244400, and Phvul.001G244500 candidate genes for further validation. In this study, the relative expression of Co-AC candidate genes was assessed, as well as other putative genes in the vicinity of this locus and known resistance genes, in the AC cultivar following inoculation with the race 73 of C. lindemuthianum. Gene expression analysis revealed significantly higher expression levels of Phvul.001G244500. Notably, Phvul.001G244500 encodes a putative Basic Helix–Loop–Helix transcription factor, suggesting its involvement in the regulation of defense responses. Furthermore, a significant modulation of the expression of defense-related genes PR1a, PR1b, and PR2 was observed in a time-course experiment. These findings contribute to the development of improved strategies for breeding anthracnose-resistant common bean cultivars, thereby mitigating the impact of this pathogen on crop yields and ensuring sustainable bean production. Full article

Animal-sourced foods are important for human nutrition and health, but they can have a negative impact on the environment. These impacts can result in land use tensions associated with population growth and the loss of native forests and wetlands during agricultural expansion. Increased greenhouse gas emissions, and high water use but poor water quality outcomes can also be associated. Life cycle analysis from cradle-to-distribution has shown that novel plant-based meat alternatives can have an environmental footprint lower than that of beef finished in feedlots, but higher than for beef raised on well-managed grazed pastures. However, several technologies and practices can be used to mitigate impacts. These include ensuring that grazing occurs when feed quality is high, the use of dietary additives, breeding of animals with higher growth rates and increased fecundity, rumen microbial manipulations through the use of vaccines, soil management to reduce nitrous oxide emission, management systems to improve carbon sequestration, improved nutrient use efficacy throughout the food chain, incorporating maize silage along with grasslands, use of cover crops, low-emission composting barns, covered manure storages, and direct injection of animal slurry into soil. The technologies and systems that help mitigate or actually provide solutions to the environmental impact are under constant refinement to enable ever-more efficient production systems to allow for the provision of animal-sourced foods to an ever-increasing population. Full article

An oxidizing and harmful pollutant gas, tropospheric ozone is a product of a complex set of photochemical reactions that can make it difficult to enact effective control measures. A better understanding of its precursors including volatile organic compounds (VOCs) and nitrogen oxides (NO_x) and their spatial distribution can enable policymakers to focus their control efforts. In this study we used low-cost sensors (LCSs) to increase the spatial resolution of an existing NO₂ monitoring network in addition to VOC sampling to better understand summer ozone formation in Maricopa County, Arizona, and observed that afternoon O₃ values at the downwind sites were significantly correlated, ~0.27, to the morning NO₂ × rate values at the urban sites. Additionally, we looked at the impact of wildfire smoke on ozone exceedances and compared non-smoke days to smoke days. The average O₃ on smoke days was approximately 20% higher than on non-smoke days, however, the average NO₂ concentration multiplied by estimated photolysis rate (NO₂ × rate) values were only 2% higher on smoke days. Finally, we evaluated the ozone sensitivity of the region by calculating HCHO/NO₂ ratios using three different datasets: ground, satellite, and model. Although the satellite dataset produced higher HCHO/NO₂ ratios than the other datasets, when the proper regime thresholds are applied the three datasets consistently show transition and VOC-limited O₃ production regimes over the Phoenix metro area. This suggests a need to implement more VOC emission controls in order to reach O₃ attainment in the county. Full article

The adaptive potential of plants in urban environments, responding to factors like air pollution, electromagnetic radiation, and specific microclimates, remains insufficiently understood. Our study focused on two evergreen Cupressaceae family species, Thuja occidentalis L. and Platycladus orientalis L. Franco, which are commonly found in Kazakhstan’s urban landscapes. Conducted in Almaty, one of Kazakhstan’s most polluted cities, our comparative analysis examined the anatomical features, photosynthetic activity, and secondary metabolite composition of these conifers. Both species exhibited xeromorphic traits, such as submerged stomata, resin passages, and a prominent leaf cuticle. T. occidentalis displayed higher photosynthetic activity values (quantum yield of photosystem II (YII), electron transport rate (ETR), and quantum yield of non-photochemical quenching (Y(NPQ))) than P. orientalis, while P. orientalis exhibited a higher quantum yield of non-regulated energy dissipation in PSII (Y(NO)) values. Chemical analysis revealed 31 components in T. occidentalis and 33 in P. orientalis, with T. occidentalis containing three times more thujone (16.42% and 5.18%, respectively) and a higher monosaccharide content (17.33% and 6.98%, respectively). T. occidentalis also contained 14.53% steroids, whereas P. orientalis showed no steroid presence. The cytotoxic activity of essential oils was determined by the survival of Artemia salina aquatic crustaceans, whereas tested essential oils from both species exhibited acute lethal toxicity to A. salina aquatic crustaceans across all tested concentrations. The connection between physiological traits, adaptation strategies, and cytotoxic effects offers a comprehensive view of the ecological and pharmacological importance of these two observed conifer species, highlighting their diverse roles in urban environments, as well as their potential medical uses. Full article

The qualitative impact of pollutants on water quality is mainly related to their nature and their concentration, but in any case, they determine a strong impact on the involved ecosystems. In particular, refractory organic compounds represent a critical challenge, and several degradation processes have been studied and developed for their removal. Among them, heterogeneous Fenton treatment is a promising technology for wastewater and liquid waste remediation. Here, we have developed mono- and bimetallic formulations based on Co, Cu, Fe, and Mn, which were investigated for the degradation of three model organic dyes (methylene blue, rhodamine B, and malachite green). The treated samples were then analyzed by means of UV-vis spectrophotometry techniques. Bimetallic iron-based materials achieved almost complete degradation of all three model molecules in very short time. The Mn-Fe catalyst resulted in the best formulation with an almost complete degradation of methylene blue and malachite green at pH 5 in 5 min and of rhodamine B at pH 3 in 30 min. The results suggest that these formulations can be applied for the treatment of a broad range of liquid wastes comprising complex and variable organic pollutants. The investigated catalysts are extremely promising when compared to other systems reported in the literature. Full article

Planar light concentrators are potential applications for solar thermal conversion, in which the intensity of the electric field will exhibit strongly non-uniform characteristics. However, previous research has long ignored the solar absorption performance of plasmonic nanoparticles in the focused electric field. In this work, we use the finite element method (FEM) to study the optical behaviors of a single nanoparticle and multiple nanoparticles in the focused electric field formed by vertically and inwardly imposing the initial incident light on a quarter cylindrical surface. The results show that the focused electric field can significantly improve the solar absorption abilities compared with the parallel one for all the nanoparticles due to the local near-electric field enhancement caused by the aggregation of the free electrons on the smaller zone. Further studies on the focused electric field reveal that the plasmon heating behavior of Au spheres presents a rising trend with the decrease in inter-particle spacing, as the gap is less than the radius of Au spheres. As the number of nanoparticles increases along the focal line, the absorption power of the center nanoparticles gradually tends to be stable, and it is much lower than that of a single nanoparticle. As the nanoparticles are arranged along the y and z directions, the heterogeneity of the electric field makes the optical properties uneven. Notably, the strongest electric field appears slightly close to the incident surface rather than on the focal line. Full article

One of the most promising areas of research in palaeomicrobiology is the study of the human microbiome. In particular, ancient dental calculus helps to reconstruct a substantial share of oral microbiome composition by mapping together human evolution with its state of health/oral disease. This review aims to trace microbial characteristics in ancient dental calculus to describe the evolution of the human host-oral microbiome relationship in oral health or disease in children and adults. Following the PRISMA-Extension for Scoping Reviews guidelines, the main scientific databases (PubMed, Scopus, Lilacs, Cochrane Library) have been drawn upon. Eligibility criteria were established, and all the data collected on a purpose-oriented collection form were analysed descriptively. From the initial 340 records, only 19 studies were deemed comprehensive enough for the purpose of this review. The knowledge of the composition of ancient oral microbiomes has broadened over the past few years thanks to increasingly well-performing decontamination protocols and additional analytical avenues. Above all, metagenomic sequencing, also implemented by state-of-the-art bioinformatics tools, allows for the determination of the qualitative-quantitative composition of microbial species associated with health status and caries/periodontal disease. Some microbial species, especially periodontal pathogens, do not appear to have changed in history, while others that support caries disease or oral health could be connected to human evolution through lifestyle and environmental contributing factors. Full article

Obesity results in hepatic fat accumulation, i.e., steatosis. In addition to fat overload, impaired fatty acid β-oxidation also promotes steatosis. Fatty acid β-oxidation takes place in the mitochondria and peroxisomes. Usually, very long-chain and branched-chain fatty acids are the first to be oxidized in peroxisomes, and the resultant short chain fatty acids are further oxidized in the mitochondria. Peroxisome biogenesis is regulated by peroxin 16 (PEX16). In liver-specific PEX16 knockout (Pex16^Alb-Cre) mice, hepatocyte peroxisomes were absent, but hepatocytes proliferated, and liver mass was enlarged. These results suggest that normal liver peroxisomes restrain hepatocyte proliferation and liver sizes. After high-fat diet (HFD) feeding, body weights were increased in PEX16 floxed (Pex16^fl/fl) mice and adipose-specific PEX16 knockout (Pex16^AdipoQ-Cre) mice, but not in the Pex16^Alb-Cre mice, suggesting that the development of obesity is regulated by liver PEX16 but not by adipose PEX16. HFD increased liver mass in the Pex16^fl/fl mice but somehow reduced the already enlarged liver mass in the Pex16^Alb-Cre mice. The basal levels of serum triglyceride, free fatty acids, and cholesterol were decreased, whereas serum bile acids were increased in the Pex16^Alb-Cre mice, and HFD-induced steatosis was not observed in the Pex16^Alb-Cre mice. These results suggest that normal liver peroxisomes contribute to the development of liver steatosis and obesity. Full article

Biodegradable polymers have been extensively researched in the field of biomedicine. Polylactic-co-glycolic acid (PLGA), a biodegradable polymer material, has been widely used in drug delivery systems and has shown great potential in various medical fields, including vaccines, tissue engineering such as bone regeneration and wound healing, and 3D printing. Cancer, a group of diseases with high mortality rates worldwide, has recently garnered significant attention in the field of immune therapy research. In recent years, there has been growing interest in the delivery function of PLGA in tumor immunotherapy. In tumor immunotherapy, PLGA can serve as a carrier to load antigens on its surface, thereby enhancing the immune system’s ability to attack tumor cells. Additionally, PLGA can be used to formulate tumor vaccines and immunoadjuvants, thereby enhancing the efficacy of tumor immunotherapy. PLGA nanoparticles (NPs) can also enhance the effectiveness of tumor immunotherapy by regulating the activity and differentiation of immune cells, and by improving the expression and presentation of tumor antigens. Furthermore, due to the diverse physical properties and surface modifications of PLGA, it has a wider range of potential applications in tumor immunotherapy through the loading of various types of drugs or other innovative substances. We aim to highlight the recent advances and challenges of plga in the field of oncology therapy to stimulate further research and development of innovative PLGA-based approaches, and more effective and personalized cancer therapies. Full article

As an outgrowth of globalization, religious globalization has significantly transformed the religious landscape worldwide. Contemporary societies exhibit religious pluralism, posing challenges for services such as spiritual or pastoral care. This study aimed to investigate how pastoral/spiritual caregivers of divergent cultural and religious backgrounds navigate religious diversity and how their religious location influences their inter-religious relations. Data were gathered through a standardized open-ended interview protocol. The study sample consisted of German Christian pastoral caregivers and Turkish Muslim spiritual caregivers from Germany and Turkey, respectively (N = 67). Overall, the entire sample expressed a generally positive attitude towards providing spiritual/pastoral care (S/PC) to individuals of other religious affiliations. German participants emphasized a human-centered approach towards individuals from diverse religious and cultural backgrounds, whereas Turkish participants placed greater emphasis on the qualifications of the caregiver. Turkish participants exhibited less exposure to other cultures/religions compared to their German counterparts, yet both subsamples responded positively to requests for care from individuals of different faiths. Both subsamples adhered to standard procedures during S/PC visits. German participants were more inclined to incorporate elements from other religions/cultures into their S/P work compared to Turkish participants. The majority of participants regarded their respective institutions (Church/Diyanet) as responsible for addressing the spiritual needs of others. However, the German subsample displayed greater reluctance towards the employment of pastoral caregivers from different religious backgrounds by their institution, as opposed to the Turkish subsample. Full article

In this study, a novel system which integrates solar thermal energy with membrane gas absorption technology is proposed to capture CO₂ from a 580 MWe pulverized coal power plant. Technical feasibility and economic evaluation are carried out on the proposed system in three cities with different solar resources in China. Research results show that the output capacity and net efficiency of the SOL-HFMC power plant are significantly higher than those of the reference power plant regardless of whether a TES system is applied or not. In addition, the CEI of the SOL-HFMC power plant with the TES system is 4.36 kg CO₂/MWh, 4.45 kg CO₂/MWh and 4.66 kg CO₂/MWh lower than that of the reference power plant. The prices of the membrane, vacuum tube collector and phase change material should be reduced to achieve lower LCOE and COR values. Specifically for the SOL-HFMC power plant with the TES system, the corresponding vacuum tube collector price shall be lower than 25.70 $/m² for Jinan, 95.20 $/m² for Xining, and 128.70 $/m² for Lhasa, respectively. To be more competitive than a solar-assisted ammonia-based post-combustion CO₂ capture power plant, the membrane price in Jinan, Xining and Lhasa shall be reduced to 0.012 $/m, 0.015 $/m and 0.016 $/m for the sake of LCOE, and 0.03 $/m, 0.033 $/m and 0.034 $/m for the sake of COR, respectively. Full article

Low-fidelity analytic and computational wave drag prediction methods assume linear aerodynamics and small perturbations to the flow. Hence, these methods are typically accurate for only very slender geometries. The present work assesses the accuracy of these methods relative to high-fidelity Euler, compressible computational-fluid-dynamics solutions for a set of axisymmetric geometries with varying radius-to-length ratios $(R/L)$. Grid-resolution studies are included for all computational results to ensure grid-resolved results. Results show that the low-fidelity analytic and computational methods match the Euler CFD predictions to around a single drag count ( ∼$1.0\times {10}^{-4}$) for geometries with $R/L\le 0.05$ and Mach numbers from 1.1 to 2.0. The difference in predicted wave drag rapidly increases, to over 30 drag counts in some cases, for geometries approaching $R/L\approx 0.1$, indicating that the slender-body assumption of linear supersonic theory is violated for larger radius-to-length ratios. All three methods considered predict that the wave drag coefficient is nearly independent of Mach number for the geometries included in this study. Results of the study can be used to validate other numerical models and estimate the error in low-fidelity analytic and computational methods for predicting wave drag of axisymmetric geometries, depending on radius-to-length ratios. Full article

Wavelet decomposition is pivotal for underwater image processing, known for its ability to analyse multi-scale image features in the frequency and spatial domains. In this paper, we propose a new biorthogonal cubic special spline wavelet (BCS-SW), based on the Cohen–Daubechies–Feauveau (CDF) wavelet construction method and the cubic special spline algorithm. BCS-SW has better properties in compact support, symmetry, and frequency domain characteristics. In addition, we propose a K-layer network (KLN) based on the BCS-SW for underwater image enhancement. The KLN performs a K-layer wavelet decomposition on underwater images to extract various frequency domain features at multiple frequencies, and each decomposition layer has a convolution layer corresponding to its spatial size. This design ensures that the KLN can understand the spatial and frequency domain features of the image at the same time, providing richer features for reconstructing the enhanced image. The experimental results show that the proposed BCS-SW and KLN algorithm has better image enhancement effect than some existing algorithms. Full article

This study offers a comprehensive insight into the realms of economic fraud and risk management, underscoring the necessity of adaptability to evolving technologies and shifts in financial market dynamics. Through the application of bibliometric methodologies, this study meticulously maps the relevant literature, delineating influential works, notable authors, collaborative networks, and emerging trends. It reviews key research contributions within the field, alongside reputable journals and institutions engaged in academic research. The examination highlights the logical, conceptual, and social interconnections that define the landscape of economic fraud and associated risks, elucidating how these findings inform the understanding, mitigating, and combating of the risk of fraud. Our bibliometric analysis methodology is grounded in the utilization of the Scopus database, employing rigorous filtering and extraction processes to obtain a substantial corpus of pertinent articles. Through a fusion of performance analysis and science mapping, our investigation elucidates central themes and visually represents the interrelationships between studies. Our research outcomes underscore the frequency of paper publications across diverse regions, with particular emphasis on the predominant scientific output from the US and China. Additionally, trends in academic citations are identified, indicative of the significant impact of papers on academic research and the formulation of public policies. By means of bibliometric analysis, this study not only consolidates existing knowledge but also catalyzes the exploration of future research trajectories, emphasizing the imperative of addressing these issues with heightened scientific rigor. Full article

Human amoebiasis still represents a major health problem worldwide. Metronidazole has been used as the most common drug to treat the disease; however, it is also known that the drug causes undesirable side effects. This has led to the search for new pharmacological alternatives which include phytochemical compounds with antiamoebic effects. We analyzed the amoebicidal activity of stevioside (STV), a diterpene glycoside present in Stevia rebaudiana, on trophozoites of E. histolytica. Different concentrations of STV were tested, and an inhibitory concentration of 50% of cell viability (IC₅₀) was determined with an exposition of 9.53 mM for 24 h. Trophozoites exposed to STV showed morphological changes evidenced by the decrease in the basic structures related to the movement and adherence to the substrate, as well as ultrastructural features characterized by a loss of regularity on the cell membrane, an increase in cytoplasmic granularity, and an increase in apparent autophagic vacuoles. Also, the decrease in cysteine protease expression and the proteolytic activity of trophozoites to degrade the cell monolayer were analyzed. A histological analysis of hamster livers inoculated with trophozoites and treated with STV showed changes related to the granulomatous reaction of the liver parenchymal tissue. Our results constitute the first report related to the possible use of STV as a therapeutic alternative in amoebiasis. Full article

The looking-down mode of space airship bistatic radars faces complex sea–land clutter, and the mode of wide-range surveillance and the over-sight detection of the satellite platform generates a low SNR and range–Doppler ambiguity. The method traditionally used involves the transmission of multiple Pulse Repetition Frequencies (PRFs) and correlating them to solve the ambiguity. However, with a low SNR, the traditional disambiguation fails due to the large number of false alarms and target omissions. In order to solve this problem, a new algorithm for multi-target joint detection and range–Doppler disambiguation based on an ambiguity matrix is presented. Firstly, all possible state values corresponding to the ambiguous sequence are filled into the ambiguity matrix one by one. Secondly, the state values in the matrix cell are divided into several groups of subsequences according to the PRF. By disambiguating multiple sets of subsequences, performing subsequence fusion, and then undertaking point aggregation, the targets can be effectively detected in scenarios with a strong clutter rate, the false alarms can be suppressed, and the disambiguation of the range and Doppler is completed. The simulation shows that the proposed algorithm has the strong ability to detect targets and perform ambiguity resolution in the scenario of a multi-target and multi-false alarm. Full article