Wolbachia, a group of Gram-negative symbiotic bacteria, infects nematodes and a wide range of arthropods. Diaphorina citri Kuwayama, the vector of Candidatus Liberibacter asiaticus (CLas) that causes citrus greening disease, is naturally infected with Wolbachia (wDi). However, the interaction between wDi and D. citri remains poorly understood. In this study, we performed a pan-genome analysis using 65 wDi genomes to gain a comprehensive understanding of wDi. Based on average nucleotide identity (ANI) analysis, we classified the wDi strains into Asia and North America strains. The ANI analysis, principal coordinates analysis (PCoA), and phylogenetic tree analysis supported that the D. citri in Florida did not originate from China. Furthermore, we found that a significant number of core genes were associated with metabolic pathways. Pathways such as thiamine metabolism, type I secretion system, biotin transport, and phospholipid transport were highly conserved across all analyzed wDi genomes. The variation analysis between Asia and North America wDi showed that there were 39,625 single-nucleotide polymorphisms (SNPs), 2153 indels, 10 inversions, 29 translocations, 65 duplications, 10 SV-based insertions, and 4 SV-based deletions. The SV-based insertions and deletions involved genes encoding transposase, phage tail tube protein, ankyrin repeat (ANK) protein, and group II intron-encoded protein. Pan-genome analysis of wDi contributes to our understanding of the geographical population of wDi, the origin of hosts of D. citri, and the interaction between wDi and its host, thus facilitating the development of strategies to control the insects and huanglongbing (HLB). Full article

This study investigates polyethylene glycol (PEG) homopolymer thin film adsorption on gold surfaces of controlled surface chemistry. The conformational states of physisorbed PEG are analyzed through polarization modulation infrared reflection–absorption spectrometry (PM-IRRAS). The PM-IRRAS principle is based on specific optical selection rules allowing the detection of surface-specific FTIR response of thin polymer films on the basis of differential reflectivity at the polymer/substrate interface for p- and s-polarized light. The intensification of the electric field generated at the PEG/substrate interface for p-polarized IR light in comparison with s-polarized light permits the analysis of PEG chain anisotropy and conformational changes induced by the adsorption. Results showed that PEG adsorbs on model substrates having a rather hydrophilic character in a way that the PEG chains spread parallel to the surface. In the case of a very hydrophilic substrate, the adsorbed PEG chains are in a stable thermodynamic state which allows them to arrange and crystallize as stacked crystalline lamellae after adsorption. The surface topography and morphology of the PEG thin films were also investigated by atomic force microscopy (AFM). While in the bulk state, PEG crystallizes in the form of large spherulites; on substrates whose adsorption is favored by surface chemistry, PEG crystallizes in the form of stacked lamellae with a thickness equal to 20 nm. Conversely, on a hydrophobic substrate, the PEG chains do not crystallize and adsorption occurs in the statistical coil state. Full article

Rice straw presents challenges as livestock feed due to its low digestibility and the presence of chemical residues. One potential solution is to focus on breeding brittle varieties that possess disease-resistance traits. In this study, AZ1803, a brittle mutant line isolated from the IR64 mutant pool, was chosen for gene identification and breeding. The AZ1803 mutant was crossed to the TNG67 variety to generate a mapping population and to the CS11 variety for fine mapping and breeding. The gene was mapped on chr. 10 between RM467 and RM171 SSR markers and was narrowed down to RM271 and RM5392 with 600 kb proximately interval. The AZ1803 and IR64 sequencing results revealed a substitution mutant in the Exon 9th of the OsCesA7 gene, resulting in an amino acid mutation at the end of the transmembrane domain 5th of the CESA7, responsible for cellulose synthesis for the secondary cell wall. The cellulose content of AZ1803 was reduced by 25% compared with the IR64. A new brittle and disease-resistant variety was bred by using developed markers in marker-assisted selection. In addition, bending tests and bacterial blight inoculation were applied. The bacterial lesion length of the bred variety is 64% lower than that of AZ1803. The rice straw of the new variety can be used for livestock feeding, which increases farmer income and reduces pesticide residues and air pollution from straw burning. Full article

The jet dynamics during cavitation bubble collapsing between unequal-sized dual particles are investigated utilizing a numerical model that combines the finite volume approach alongside the volume of fluid approach. The model incorporates the compressibility of the two-phase fluid and accounts for mass and heat transfer between two phases. The computational model utilizes an axisymmetric model, where the axis of symmetry is defined as the line that connects the centers of the particles and the bubble. A comprehensive analysis is presented on the influence of the particle radius and bubble–particle distance on the jet behavior. Furthermore, the variations of surface pressure on the particles induced by jet impingement are quantitatively analyzed. Four distinct jet behaviors are categorized, depending on the formation mechanism, as well as the number and the direction of the jets. For case 1, the bubble produces a single jet directed toward a small particle; for case 2, the bubble fragments produces double jets receding from each other; for case 3, the bubble produces double jets approaching each other; and for case 4, the bubble produces a single jet directed toward a large particle. The pressure perturbations induced by jet impingement upon the particles exceed those caused by shock wave impacts. The larger the bubble volume at the moment of jet formation, the longer the duration of the pressure variation caused by the jet impinging on the particles. Full article

Designs for remanufacturing (DfRem) consider the remanufacturability of the product in the early stages of product design, which can greatly increase the reusability of the products. However, product design schemes lack reasonable evaluation indicators for remanufacturability, and the decision-makers of the design scheme have subjective preferences and vague hesitation. These result in inaccurate decision making on DfRem schemes that will affect the successful implementation of product remanufacturing. In order to improve the accuracy of the DfRem scheme decision, a fuzzy decision-making method for green design for remanufacturability is proposed. Firstly, an evaluation indicator system for green design schemes was established that takes into account remanufacturability, reliability, cost, and the environment, and the entropy weighting method is used to quantify and weigh the design scheme evaluation indicators. Then, the hesitation fuzzy set is applied to construct the set of evaluations and the optimal design scheme is selected by applying the comprehensive evaluation method. Finally, the feasibility of the above method is verified by using the green design of an injection mold as an example, and the results show that the above method is able to make accurate and effective design scheme decisions. This method has been implemented in a prototype system using Visual Studio 2022 and Microsoft SQL Server2022. The results show that the fuzzy decision-making system is accurate and effective for rapidly generating a rational green design scheme for remanufacturability. Full article

Membrane contactors are among the available technologies that allow a reduction in the amount of ammoniacal nitrogen released into the environment through a process called transmembrane chemical absorption (TMCA). This process can be operated with different substances acting as trapping solutions; however, strong inorganic acids have been studied the most. The purpose of this study was to demonstrate, at laboratory scale, the performance of citric acid as a capturing solution in TMCA processes for recovering ammonia as an organic fertilizer from anaerobic digestor reject water using membrane contactors in a liquid–liquid configuration and to compare it with the most studied solution, sulfuric acid. The experiments were carried out at 22 °C and 40 °C and with a feed water pH of 10 and 10.5. When the system was operated at pH 10, the rates of recovered ammonia from the feed solution obtained with citric acid were 10.7–16.5 percentage points (pp) lower compared to sulfuric acid, and at pH 10.5, the difference decreased to 5–10 pp. Under all tested conditions, the water vapor transport in the system was lower when using citric acid as the trapping solution, and at pH 10 and 40 °C, it was 5.7 times lower. When estimating the operational costs for scaling up the system, citric acid appears to be a better option than sulfuric acid as a trapping solution, but in both cases, the process was not profitable under the studied conditions. Full article

Global climate change has exacerbated alterations in urban thermal environments, significantly impacting the daily lives and health of city residents. Measuring and understanding urban land surface temperatures (LST) and their influencing factors is important in addressing global climate change and enhancing the well-being of residents. However, due to limitations in data precision and analytical methods, existing studies often overlook the microscale examination closely related to residents' daily lives, and lack a deep exploration of the spatial heterogeneity of the influencing factors. This leads to these results being ineffective in guiding the planning and construction of cities. Taking Shenzhen as a case study, our study investigates the effects of various microscale build environment characteristics of LST using street view images and machine learning. A convolutional neural network model adopting the SegNet architecture is used to perform semantic segmentation on street view images, extracting features of the microscale urban-built environment. The LST is inverted through the Google Earth Engine (GEE) platform. By using Multiscale Geographically Weighted Regression (MGWR) models, our study reveals the comprehensive impact of the urban-built environment on LST and its significant spatial heterogeneity. The findings indicate that the proportions of sky, roads, and buildings are positively correlated with LST, while trees have a significant cooling effect. Although earth and water can reduce LST, their overall contribution is minimal due to limitations in their area and distribution patterns. This study not only reveals the key factors affecting urban LST at the microscale but also emphasizes the necessity of considering the spatial heterogeneity of these factors' impacts. This suggests the need for targeted strategies for different areas to effectively improve the urban thermal environment and achieve sustainable urban development. Full article

Simulated (sim) racing is an emerging esport that has garnered much interest in recent years and has been a relatively under-researched field in terms of expertise and performance. When examining expertise, visual attention has been of particular interest to researchers, with eye tracking technology commonly used to assess visual attention. In this study, we examined the overt visual attention allocation of high- and low-skilled sim racers during a time trial task using Tobii 3 glasses. In the study, 104 participants were tested on one occasion, with 88 included in the analysis after exclusions. Participants were allocated to either group according to their fastest lap times. Independent t-tests were carried out with sidak corrections to test our hypotheses. Our results indicate that when eye tracking metrics were normalised to the lap time and corner sector time, there was a difference in the relative length of overt attention allocation (fixation behaviour) as lower-skilled racers had significantly greater total fixation durations in laps overall and across corner sectors when normalised (p = 0.013; p = 0.018). Interestingly, high- and low-skilled sim racers differed in where they allocated their attention during the task, with high-skilled sim racers allocating significantly less overt attention to the track relative to other areas of the display (p = 0.003). This would allow for higher-skilled racers to obtain relatively more information from heads-up display elements in-game, all whilst driving at faster speeds. This study provides evidence that high-skilled sim racers appear to need significantly less overt attention throughout a fast lap, and that high- and low-skilled sim racers differ in where they allocate their attention while racing. Full article

Bacteriophages are currently considered one of the most promising alternatives to antibiotics under the ‘One Health’ approach due to their ability to effectively combat bacterial infections. This study aimed to characterize Vibrio species in hatchery water samples collected from an aquaculture farm and investigate the biocontrol potential of their bacteriophages. Vibrio spp. (n = 32) isolates confirmed by LNA probe-based qPCR were used as hosts. Three Vibrio phages were isolated. IKEM_vK exhibited a broad host range, infecting V. harveyi (n = 8), V. alginolyticus (n = 2), V. azureus (n = 1), and V. ordalii (n = 1). IKEM_v5 showed lytic activity against V. anguillarum (n = 4) and V. ordalii (n = 1), while IKEM_v14 was specific to V. scophtalmi (n = 4). The morphological appearance of phages and their lytic effects on the host were visualized using scanning electron microscopy (SEM). All three phages remained relatively stable within the pH range of 6–11 and up to 60 °C. The lytic activities and biofilm inhibition capabilities of these phages against planktonic Vibrio cells support their potential applications in controlling vibriosis in aquaculture systems. Full article

Due to immunosuppression, transplant recipients are at higher risk of infections with SARS-CoV-2 and worse clinical outcomes than immunocompetent hosts. Furthermore, lung transplant patients represent a special group among solid organ recipients, since pneumonia is the main manifestation of COVID-19. However, data on the course of disease and the changes in morbidity and mortality during the course of the pandemic are limited. In our pulmonary rehabilitation clinic, we treat patients shortly after lung transplant as well as long-term transplant patients. Over the last almost 4 years of pandemic, we witnessed several COVID-19 infections in lung transplant patients in our clinic as well as patients who acquired an infection beforehand. In this paper, we aim at retrospectively describing a series of recent COVID-19 cases in our clinic, looking at the clinical course of disease and outcomes in lung transplant patients. Full article

The only currently active industrial-scale plant that uses coal mine brines, located in Czerwionka-Leszczyny, uses ZOD (Zakład Odsalania Dębieńsko, the name of the plant’s former owner) technology, based on mechanical vapor compression evaporators. The plant produces evaporated salt that meets the specifications for edible salt; however, the technology is highly energy-consuming. The presented work focuses on the modeling of ZOD technology if applied to the water treatment of the ‘Ziemowit-650’ coal mine. Using the results of bench-scale investigation of brine nanofiltration and a mathematical model of ZOD technology based on Czerwionka-Leszczyny performance, the energy consumption per ton of produced salt was estimated for two cases: (1) ZOD technology treating the ‘Ziemowit-650’ brine and (2) ZOD technology treating the permeate of nanofiltration (NF) working on the ‘Ziemowit-650’ brine. The sensitivity of the system was investigated in the range of −10% to + 10% of Cl⁻, SO₄²⁻, Mg²⁺, and Ca²⁺ concentration, assuming that the sodium concentration also changes to meet the electroneutrality requirement. The results show that nanofiltration pretreatment not only decreases energy consumption but it also makes salt production less sensitive to fluctuations in feed water composition. Full article

This research study focused on the effect of adding boric acid to epoxy resin in order to obtain a composite material with improved properties and performance. To this end, a fine powder of boric acid (H₃BO₃) was introduced into epoxy resin in different amounts, i.e., 0.5 g, 1.0 g, and 1.5 g. As the matrix of the epoxy composites, styrene-modified epoxy resin based on bisphenol A (BPA) (Epidian 53) was used. It was cross-linked with two types of curing agents, i.e., an amine (ET) and a polyamide (PAC). The mechanical properties of the obtained epoxy composites (in terms of compressive strength, compressive modulus, and compressive strain) were determined at room temperature in order to assess the effect of the addition of boron acid and of the type of curing agent employed to cure the epoxy on these characteristics. Calorimetric measurements were made to highlight any changes in the glass transition temperature (Tg) as a result of the addition of boric acid to epoxy resin. Finally, flammability tests were performed on both Epidian 53/PAC and Epidian 53/ET epoxy composites to analyze their fire behavior and consequently establish the effectiveness of the selected additive as a flame retardant. Full article

This study investigates the association between epigenetic age acceleration (EAA) derived from DNA methylation and the risk of incident colorectal cancer (CRC). We utilized data from a random population sample of 9,360 individuals (men and women, aged 45-69) from the HAPIEE Study who had been followed up for 16 years. A nested case–control design yielded 35 incident CRC cases and 354 matched controls. Six baseline epigenetic age (EA) measures (Horvath, Hannum, PhenoAge, Skin and Blood (SB), BLUP, and Elastic Net (EN)) were calculated along with their respective EAAs. After adjustment, the odds ratios (ORs) for CRC risk per decile increase in EAA ranged from 1.20 (95% CI: 1.04-1.39) to 1.44 (95% CI: 1.21-1.76) for the Horvath, Hannum, PhenoAge, and BLUP measures. Conversely, the SB and EN EAA measures showed borderline inverse associations with ORs of 0.86-0.87 (95% CI: 0.76-0.99). Tertile analysis reinforced a positive association between CRC risk and four EAA measures (Horvath, Hannum, PhenoAge, and BLUP) and a modest inverse relationship with EN EAA. Our findings from a prospective population-based-case-control study indicate a direct association between incident CRC and four markers of accelerated baseline epigenetic age. In contrast, two markers showed a negative association or no association. These results warrant further exploration in larger cohorts and may have implications for CRC risk assessment and prevention. Full article

This article is devoted to the study of the effect of ZnO nanoparticles on the development of tolerance to salt stress in rye samples. As a quantitative criterion for assessing the degree of oxidative stress, the amount of H₂O₂ released in the samples during growth was determined. For these purposes, an electrochemical sensor based on hydrothermally synthesized wall-shaped NiO nanostructures was developed. This sensor has been proven to demonstrate high sensitivity (2474 µA·mM⁻¹), a low limit of detection (1.59 µM), good selectivity against common interferents, and excellent long-term stability. The investigation reveals that the incorporation of ZnO nanoparticles in irrigation water notably enhances rye’s ability to combat salt stress, resulting in a decrease in detected H₂O₂ levels (up to 70%), coupled with beneficial effects on morphological traits and photosynthetic rates. Full article

This paper addresses challenges in the application of existing colters in Chinese ecological tea plantations due to abundant straw roots and insufficient tillage depth. Aligned with the agronomic requirements of hilly eco-tea plantations, our study optimizes the structural advantages of the joint use of rotary tillage blades and double-disc colters to design an efficient trenching device. Our investigation explores the motion characteristics of a double-disc colter during deep trenching operations, in conjunction with rotary tillage blades. Employing discrete element method (DEM) simulations, this paper aims to minimize the working resistance and enhance the tillage depth stability. Single-factor experiments are conducted to determine the impact of key structural parameters on the tillage depth stability and working resistance. The optimal parameters are determined as a relative height of 80 mm to 120 mm, a 280 mm to 320 mm diameter for the double-disc colter, and a 10° to 14° angle between the two discs. The central composite design method is used to optimize the structural parameters of the double-disc colter. The results indicate that when the relative height is 82 mm, the diameter of the double-disc colter is 297 mm, and the angle between the two discs is 14°, the tillage depth stability performance reaches 91.64%. With a working resistance of merely 93.93 N, the trenching device achieves optimal operational performance under these conditions. Field validation testing shows a tillage depth stability coefficient of 92.37% and a working resistance of 104.2 N. These values deviate by 0.73% and 10.93%, respectively, from the simulation results, confirming the reliability of the simulation model. A field validation test further confirms that the operational performance of the colter aligns with the agronomic requirements of ecological tea plantations, offering valuable insights for research on trenching devices in such environments. Full article

Recently, sustainable development practices have increased attention as climate change and environmental impacts have increased. Interventions to encourage sustainability awareness are developing, so fostering them through education is crucial. Evidence-based studies conducted in this field have suggested the use of different digital tools to promote environmental learning gains and to foster better sustainability awareness among students. Following the PRISMA method, we found 21 articles published between 2013 and 2023 showing an interest in the use of digital tools in environmental education to foster sustainability awareness among learners. Findings indicate that virtual reality tools and climate change topics are the most trending in this research area. Further, the results show a positive impact of the use of digital tools on students’ concern for the sustainability of the planet. Full article

Penicillium digitatum and Penicillium italicum are responsible for citrus green and blue moulds (GM and BM), respectively, which are major citrus postharvest diseases. The aim of this study was to develop an optimal dipping mixture of an aqueous solution of different food additives: sodium bicarbonate (SB), sodium benzoate (SBen), and potassium sorbate (PS), in combination with heat, to control GM and BM using response surface methodology. The ranges of SB (0.0%, 3.0%, 6.0%), SBen (0.0%, 0.5%, 1.0%), PS (0.0, 0.5%, 1.0%) and temperature (20 °C, 35 °C, 50 °C) with a dipping time of 60s were tested for their impact on GM and BM on artificially inoculated oranges. Within these tested ranges, SB reduced GM severity and incidences of both GM and BM. PS affected BM severity and incidence, but not GM. SBen and temperature did not have impact on GM and BM. The most suitable food additive concentrations were identified to be 4.7% SB, 1.0% SBen and 0.7% PS, with a dipping solution temperature of 50 °C. This treatment was shown to reduce GM and BM incidence from 85 and 86% on control fruit dipped in tap water at 20 °C to 3 and 10%, respectively. Additionally, the severity of GM and BM was reduced from 64 and 26 mm on control fruit to <1 and 2.8 mm, respectively. Full article

The thermodynamic modeling of waste oil (WO) gasification by a high-temperature gasification agent (GA) composed of an ultra-superheated H₂O/CO₂ mixture is carried out. The GA is assumed to be obtained by the gaseous detonation of fuel–oxidizer–diluent mixture in a pulsed detonation gun (PDG). N-hexadecane is used as a WO surrogate. Methane or the produced syngas (generally a mixture of H₂, CO, CH₄, CO₂, etc.) is used as fuel for the PDG. Oxygen, air, or oxygen-enriched air are used as oxidizers for the PDG. Low-temperature steam is used as a diluent gas. The gasification process is assumed to proceed in a flow-through gasifier at atmospheric pressure. It is shown that the use of the detonation products of the stoichiometric methane–oxygen and methane–air mixtures theoretically leads to the complete conversion of WO into a syngas consisting exclusively of H₂ and CO, or into energy gas with high contents of CH₄ and C₂-C₃ hydrocarbons and an LHV of 36.7 (fuel–oxygen mixture) and 13.6 MJ/kg (fuel–air mixture). The use of the detonation products of the stoichiometric mixture of the produced syngas with oxygen or with oxygen-enriched air also allows theoretically achieving the complete conversion of WO into syngas consisting exclusively of H₂ and CO. About 33% of the produced syngas mixed with oxygen can be theoretically used for PDG self-feeding, thus making the gasification technology very attractive and cost-effective. To self-feed the PDG with the mixture of the produced syngas with air, it is necessary to increase the backpressure in the gasifier and/or enrich the air with oxygen. The addition of low-temperature steam to the fuel–oxygen mixture in the PDG allows controlling the H₂/CO ratio in the produced syngas from 1.3 to 3.4. Full article

Water and soil salinization significantly reduce crop yields. Among the strategies developed to counteract salt stress, biostimulants can maintain crop productivity, reversing its impact. In this context, there is interest in finding new substances that could act as biostimulants. Recently, the biostimulatory potential of Lemna minor L. (duckweed) extracts has been shown. This work aimed to highlight whether an extract from duckweed (Lemna extract—LE) could protect maize grown in salinity, exploring the mechanisms induced to improve crop resistance. Plants were grown by applying two concentrations of NaCl (150 and 300 mM), and some physiological, morphological, and biochemical traits were studied in control and salt-stressed samples, treated or not with LE. Salinity decreased shoots, roots, pigment, and soluble protein. LE prompted ameliorative changes at the root level and increased photosynthetic pigment and soluble protein. Furthermore, concerning the oxidative impairment provoked by salt stress, LE enhanced the cellular redox state, contrasting H₂O₂ and MDA accumulation and positively affecting the activity of superoxide dismutase (SOD—EC 1.15.1.1) and catalase (CAT—EC 1.11.1.6). The assessment of some mineral nutrients showed that LE stimulated their acquisition, especially for the highest salt dosage, explaining some benefits found for the parameters investigated. Full article

The quantitative evaluation and prediction of ecosystem service value (ESV) in the Lhasa River Basin can provide a basis for ecological environment assessment and land-use optimization and adjustment in the future. Previous studies on the ESV in the Lhasa River Basin have focused mainly on static assessment and evolution analysis based on historical data, and have not considered future development trends. Moreover, most of the current driving factors selected in land use and ESV prediction studies are homogeneous, and do not reflect the geographical and cultural characteristics of the study area well. With the Lhasa River Basin as the research focus, 20 driving factors were selected according to the characteristics of the plateau alpine area, and the land-use changes under three developmental orientations, namely, natural evolution, ecological protection, and agricultural development, were predicted for the year 2030 with the FLUS-Markov model. Based on these predictions, the values of ecosystem services were calculated, and their spatiotemporal dynamic characteristics were analyzed. The results show that the model has high accuracy in simulating land-use change in the Lhasa River Basin, with a kappa coefficient of 0.989 and an overall accuracy of 99.33%, indicating a high applicability. The types of land use in the Lhasa River basin are dominated by the existence of grassland, unused land, and forest, with a combined proportion of 94.3%. The change trends of each land-use type in the basin under the three scenarios differ significantly, with grassland, cropland, and building land showing the most significant changes. The area of cropland increased only in the agricultural development scenario; the areas of forest and grassland increased only in the ecological protection scenario; and the expansion of building land was most effectively controlled in the ecological protection scenario. The ESV increased in all three scenarios, and the spatial distribution of the ESV per unit area in the middle and lower reaches was greater than that in the upper reaches. The ESV was the greatest in the ecological protection scenario, with grasslands, forests, and water bodies contributing more to the ESV of the basin. This study provides decision-making references for the effective utilization of land resources, ecological environmental protection planning, and urban construction in the Lhasa River Basin in the future. Full article

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) induces direct cytopathic effects, complicating the establishment of low-cytotoxicity cell culture models for studying its replication. We initially developed a DNA vector-based replicon system utilizing the CMV promoter to generate a recombinant viral genome bearing reporter genes. However, this system frequently resulted in drug resistance and cytotoxicity, impeding model establishment. Herein, we present a novel cell culture model with SARS-CoV-2 replication induced by Cre/LoxP-mediated DNA recombination. An engineered SARS-CoV-2 transcription unit was subcloned into a bacterial artificial chromosome (BAC) vector. To enhance biosafety, the viral spike protein gene was deleted, and the nucleocapsid gene was replaced with a reporter gene. An exogenous sequence was inserted within NSP1 as a modulatory cassette that is removable after Cre/LoxP-mediated DNA recombination and subsequent RNA splicing. Using the PiggyBac transposon strategy, the transcription unit was integrated into host cell chromatin, yielding a stable cell line capable of inducing recombinant SARS-CoV-2 RNA replication. The model exhibited sensitivity to the potential antivirals forsythoside A and verteporfin. An innovative inducible SARS-CoV-2 replicon cell model was introduced to further explore the replication and pathogenesis of the virus and facilitate screening and assessment of anti-SARS-CoV-2 therapeutics. Full article

This study focuses on interdisciplinary approaches within mathematics and physics education. Secondary schools, particularly those specialized in scientific curricula, have opportunities to explore common topics between mathematics and physics; however, creating a coherent interdisciplinary educational experience is challenging. Adopting an interdisciplinary perspective when designing learning sequences becomes imperative. The proposed approach harnesses the power of storytelling to engage students, emphasizing the interconnectedness of subjects and humanizing the evolution of scientific ideas. This study investigates the adaptation of the Digital Interactive Storytelling in Mathematics (DIST-M) model for interdisciplinary storytelling learning sequences. It aims to explore how this model, initially developed for mathematics activities in a virtual environment, can be enriched with elements from inquiry-based learning models to integrate the experimental aspects of physics. The research presents a theoretical discussion grounded in the design of a learning sequence centered around the study of light, taking place in a non-virtual environment and approached from an interdisciplinary standpoint. It introduces hypotheses for adapting the DIST-M model to accommodate interdisciplinary storytelling sequences. One involves the incorporation of an additional phase within the DIST-M cycle, dedicated to consolidating, transferring to other contexts, and addressing variations in the concepts explored, proved, and refined in earlier phases. Full article