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Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach

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Abstract

This paper reports the complete process from secondary emblings (SE-derived plants) regeneration to acclimatization of Eucalyptus globulus and describes histocytological changes that occur in leaves from in vitro to ex vitro acclimatization for a 3-month period. After elongation, plants were transferred to pots with sterilized peat:perlite and acclimatized in a phytotron, with progressive reduction of RH and increase of light intensity. Histocytological analyses were performed in fixed material using light microscopy and ultrastructural changes followed by electron microscopy (SEM and TEM). The protocol used allowed the successful acclimatization of the emblings. Plants looked morphologically normal and FCM screening revealed no ploidy or DNA content abnormalities. Histocytological analyses showed significant changes along time, mostly in stomata shape and aperture, starch reserves, chloroplast morphology and mesophyll differentiation. This is the first report concerning emblings acclimatization to ex vitro conditions in Eucalyptus. It was clearly demonstrated that during acclimatization emblings suffered profound changes in leaf morphology in order to successfully adapt to ex vitro conditions.

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Acknowledgments

Authors thank Celbi for providing the material used in this study. Thanks are also due to José Dias for technical assistance. FCT supported the fellowship of Sónia Silva (SFRH/BD/32257/2006) and Maria Celeste Dias (SFRH/BPD/41700/2007). This study was supported by FCT Project PTDC/AGR-CFL/112996/2009.

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Authors and Affiliations

  1. CESAM, Department of Biology, Laboratory of Biotechnology and Cytomics, University of Aveiro, 3810-193, Aveiro, Portugal

    Glória Pinto, Sónia Silva, Armando Costa, Maria Celeste Dias & Conceição Santos

  2. Centre for Functional Ecology, Department of Botany, Faculty of Sciences and Technology, University of Coimbra, Calçada Martim de Freitas, 3001-455, Coimbra, Portugal

    João Loureiro

  3. Silvicaima SA Constância Sul, 2250-058, Constância, Portugal

    Clara Araújo & Lucinda Neves

Authors
  1. Glória Pinto
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  2. Sónia Silva
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  3. João Loureiro
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  4. Armando Costa
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  5. Maria Celeste Dias
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  6. Clara Araújo
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  7. Lucinda Neves
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  8. Conceição Santos
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Corresponding author

Correspondence to Conceição Santos.

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Communicated by D. Treutter.

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Pinto, G., Silva, S., Loureiro, J. et al. Acclimatization of secondary somatic embryos derived plants of Eucalyptus globulus Labill.: an ultrastructural approach. Trees 25, 383–392 (2011). https://doi.org/10.1007/s00468-010-0513-y

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  • DOI: https://doi.org/10.1007/s00468-010-0513-y

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