Abstract
Purpose
Nitrous oxide (N2O) is a potent greenhouse gas and is produced in the soil by nitrification and denitrification processes. Previous studies have reported apparent lower N2O emissions from dairy cow urine from fodder beet plants than from kale plants, although the underpinning mechanisms were poorly understood. Here, we report a laboratory incubation study to improve understanding of possible mechanisms underpinning different N2O emissions from two different forage plants, fodder beet (FB) and kale.
Materials and methods
The treatments included two urine sources from cows grazing FB or kale and two soils (FB soil and kale soil): FB soil control, FB soil + FB urine, FB soil + kale urine, kale soil control, kale soil + FB urine and kale soil + kale urine. The incubation temperature was 10 °C to simulate New Zealand autumn/winter soil temperatures.
Results and discussion
Results showed that the total N2O emissions from FB urine treatment and from urine treatments in the FB soil were lower than those from kale urine in the kale soil (P < 0.05). There was a delay in the ammonia-oxidising bacteria (AOB) growth in the FB soil + urine compared with that in kale soil with urine and this led to slower ammonia oxidation in the FB soil. Denitrifier communities were not significantly affected by the urine or soil treatments.
Conclusions
These results suggest that there are plant secondary metabolites (PSMs) in the FB urine or root exudates in the FB soil, which might have inhibited the AOB growth and the nitrification process which led to lower N2O emissions in the FB soil or urine. Further research is needed to determine the chemical composition of urine from different forage plants and to identify potential PSMs or root exudates in the urine or soil from different forage plants.
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References
Baggs EM, Smales CL, Bateman EJ (2010) Changing pH shifts the microbial source as well as the magnitude of N2O emission from soil. Biol Fert Soil 46:793–805
Bourgaud F, Gravot A, Milesi S, Gontier E (2001) Production of plant secondary metabolites: a historical perspective. Plant Sci 161:839–851
Cameron K, Di H, Moir J (2013) Nitrogen losses from the soil/plant system: a review. Ann Appl Biol 162:145–173
De Klein C, Letica S, Macfie P (2014) Evaluating the effects of dicyandiamide (DCD) on nitrogen cycling and dry matter production in a 3-year trial on a dairy pasture in South Otago, New Zealand. New Zeal J Agr Res 57:316–331
Di HJ, Cameron KC (2002) The use of a nitrification inhibitor, dicyandiamide (DCD), to decrease nitrate leaching and nitrous oxide emissions in a simulated grazed and irrigated grassland. Soil Use Manag 18:395–403
Di HJ, Cameron KC (2004) Treating grazed pasture soil with a nitrification inhibitor, eco-n™, to decrease nitrate leaching in a deep sandy soil under spray irrigation—a lysimeter study. NZ J Agric Res 47(3):351–361
Di HJ, Cameron KC (2006) Nitrous oxide emissions from two dairy pasture soils as affected by different rates of a fine particle suspension nitrification inhibitor, dicyandiamide. Biol Fertil Soils 42:472–480
Di HJ, Cameron KC (2016) Inhibition of nitrification to mitigate nitrate leaching and nitrous oxide emissions in grazed grassland: a review. J Soils Sediments 16:1401–1420
Di HJ, Cameron KC, Sherlock RR (2007) Comparison of the effectiveness of a nitrification inhibitor, dicyandiamide, in reducing nitrous oxide emissions in four different soils under different climatic and management conditions. Soil Use Manag 23:1–9
Di HJ, Cameron KC, Shen JP, Winefield C, O’Callaghan M, Bowatte S, He J (2009) Nitrification driven by bacteria and not archaea in nitrogen-rich grassland soils. Nat Geosci 2:621–624
Di HJ, Cameron KC, Shen J-P, Winefield CS, O'Callaghan M, Bowatte S, He J-Z (2010) Ammonia-oxidizing bacteria and archaea grow under contrasting soil nitrogen conditions. FEMS Microbiol Ecol 72:386–394
Di HJ, Cameron KC, Podolyan A, Robinson A (2014) Effectof soil moisture status and a nitrification inhibitor, dicyandiamide, on ammonia oxidizer and denitrifier growth and nitrous oxide emissions in a grassland soil. Soil Biol Biochem 73:59–68
Di HJ, Cameron KC, Podolyan A, Edwards GR, de Klein CA, Dynes R, Woods R (2016) The potential of using alternative pastures, forage crops and gibberellic acid to mitigate nitrous oxide emissions. J Soils Sediments 16:2252–2262
Dietz M, Machill S, Hoffmann HC, Schmidtke K (2013) Inhibitory effects of Plantago lanceolata L. on soil N mineralization. Plant Soil 368:445–458
Edwards G, De Ruiter J, Dalley D, Pinxterhuis J, Cameron K, Bryant R, Di H, Malcolm B, Chapman D (2014) Urinary nitrogen concentration of cows grazing fodder beet, kale and kale-oat forage systems in winter, Proceedings of the 5th Australasian Dairy Science Symposium, pp 144–147
Firestone MK, Davidson EA (1989) Microbiological basis of NO and N2O production and consumption in soils. In: Andreae MO, Schimel DS (eds) Exchange of trace gases between terrestrial ecosystems and the atmosphere. John Wiley & Sons, Chichester, pp 7–21
Francis CA, Roberts KJ, Beman JM, Santoro AE, Oakley BB (2005) Ubiquity and diversity of ammonia-oxidizing archaea in water columns and sediments of the ocean. Proc Natl Acad Sci U S A 102:14683–14688
Gardiner C, Clough T, Cameron K, Di H, Edwards G, de Klein C (2016) Potential for forage diet manipulation in New Zealand pasture ecosystems to mitigate ruminant urine derived N2O emissions: a review. New Zeal J Agr Res 59:301–317
Hallin S, Lindgren P-E (1999) PCR detection of genes encoding nitrite reductase in denitrifying bacteria. Appl Environ Microbiol 65:1652–1657
He JZ, Hu HW, Zhang LM (2012) Current insights into the autotrophic thaumarchaeal ammonia oxidation in acidic soils. Soil Biol Biochem 55:146–154
Hu HW, Chen D, He JZ (2015) Microbial regulation of terrestrial nitrous oxide formation: understanding the biological pathways for prediction of emission rates. FEMS Microbiol Rev 39(5):729–749
Hutchinson G, Mosier A (1981) Improved soil cover method for field measurement of nitrous oxide fluxes. Soil Sci Soc Am J 45:311–316
Jarvis SC, Schofield D, Pain B (1995) Nitrogen cycling in grazing systems. In: Bacon PE (ed) Nitrogen fertilization and the environment. Marcel Dekker, New York, pp 381–419
Jones CM, Graf DR, Bru D, Philippot L, Hallin S (2013) The unaccounted yet abundant nitrous oxide-reducing microbial community: a potential nitrous oxide sink. ISME J 7:417–426
Kloos K, Mergel A, Rösch C, Bothe H (2001) Denitrification within the genus Azospirillum and other associative bacteria. Funct Plant Biol 28:991–998
Li CY, Di HJ, Cameron KC, Podolyan A, Zhu BCH (2016) Effect of different land use and land use change on ammonia oxidiser abundance and N2O emissions. Soil Biol Biochem 96:169–175
Luo J, Ledgard S, Lindsey S (2008) A test of a winter farm management option for mitigating nitrous oxide emissions from a dairy farm. Soil Use Manag 24:121–130
Luo J, Sun XZ, Pacheco D, Ledgard S, Lindsey S, Hoogendoorn CJ, Wise B, Watkins N (2015) Nitrous oxide emission factors for urine and dung from sheep fed either fresh forage rape (Brassica napus L.) or fresh perennial ryegrass (Lolium perenne L.) Animal 9:534–543
McCarty G, Bremner J, Schmidt E (1991) Effects of phenolic acids on ammonia oxidation by terrestrial autotrophic nitrifying microorganisms. FEMS Microbiol Lett 85:345–349
MFE (2015) New Zealand’s greenhouse gas inventory 1990–2013. Ministry for the Environment, Wellington
Michotey V, Méjean V, Bonin P (2000) Comparison of methods for quantification of cytochrome cd 1-denitrifying bacteria in environmental marine samples. Appl Environ Microbiol 66:1564–1571
Mosier A, Kroeze C, Nevison C, Oenema O, Seitzinger S, van Cleemput O (1998) Closing the global N2O budget: nitrous oxide emissions through the agricultural nitrogen cycle—OECD/IPCC/IEA phase II development of IPCC guidelines for national greenhouse gas inventory methodology. Nutr Cycl Agroecosys 52:225–248
Rice EL, Pancholy SK (1974) Inhibition of nitrification by climax ecosystems. III. Inhibitors other than tannins. Am J Bot 61:1095–1103
Rotthauwe J-H, Witzel K-P, Liesack W (1997) The ammonia monooxygenase structural gene amoA as a functional marker: molecular fine-scale analysis of natural ammonia-oxidizing populations. Appl Environ Microbiol 63:4704–4712
Selbie D, Cameron K, Di H, Moir J, Lanigan G, Richards K (2014) The effect of urinary nitrogen loading rate and a nitrification inhibitor on nitrous oxide emissions from a temperate grassland soil. J Agr Sci 152:159–171
Throbäck IN, Enwall K, Jarvis Å, Hallin S (2004) Reassessing PCR primers targeting nirS, nirK and nosZ genes for community surveys of denitrifying bacteria with DGGE. FEMS Microbiol Ecol 49:401–417
Totty V, Greenwood S, Bryant R, Edwards G (2013) Nitrogen partitioning and milk production of dairy cows grazing simple and diverse pastures. J Dairy Sci 96:141–149
Acknowledgements
We would like to thank the New Zealand Agricultural Greenhouse Gas Research Centre (NZAGRC), The Catalyst Fund (Seeding) and CSC (CSC No.201503270015) for funding and Steve Moore, Carole Barlow, Jie Lei, Qian Liang and Barry Anderson of Lincoln University for the technical support.
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Responsible editor: Weixin Ding
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Yao, B., Di, H.J., Cameron, K.C. et al. Understanding the mechanisms for the lower nitrous oxide emissions from fodder beet urine compared with kale urine from dairy cows. J Soils Sediments 18, 85–93 (2018). https://doi.org/10.1007/s11368-017-1780-7
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DOI: https://doi.org/10.1007/s11368-017-1780-7