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16 pages, 5155 KiB

Open AccessArticle

Microbe-Derived Antioxidants Protect IPEC-1 Cells from H₂O₂-Induced Oxidative Stress, Inflammation and Tight Junction Protein Disruption via Activating the Nrf2 Pathway to Inhibit the ROS/NLRP3/IL-1β Signaling Pathway

by Cheng Shen, Zhen Luo, Sheng Ma, Chengbing Yu, Ting Lai, Shangshang Tang, Hongcai Zhang, Jing Zhang, Weina Xu and Jianxiong Xu

Antioxidants 2024, 13(5), 533; https://doi.org/10.3390/antiox13050533 (registering DOI) - 27 Apr 2024

Abstract

Oxidative stress can induce inflammation and tight junction disruption in enterocytes. The initiation of inflammation is thought to commence with the activation of the ROS/NLRP3/IL-1β signaling pathway, marking a crucial starting point in the process. In our previous studies, we found that microbe-derived [...] Read more.

Oxidative stress can induce inflammation and tight junction disruption in enterocytes. The initiation of inflammation is thought to commence with the activation of the ROS/NLRP3/IL-1β signaling pathway, marking a crucial starting point in the process. In our previous studies, we found that microbe-derived antioxidants (MAs) showed significant potential in enhancing both antioxidant capabilities and anti-inflammatory effects. The main aim of this research was to investigate the ability of MAs to protect cells from oxidative stress caused by H₂O₂, to reduce inflammatory responses, and to maintain the integrity of tight junction proteins by modulating the ROS/NLRP3/IL-1β signaling pathway. IPEC-1 cells (1 × 10⁴ cells/well) were initially exposed to 100 mg/L of MAs for 12 h, after which they were subjected to 1 mM H₂O₂ treatment for 1 h. We utilized small interfering RNA (siRNA) to inhibit the expression of NLRP3 and Nrf2. Inflammatory factors such as IL-1β and antioxidant enzyme activity levels were detected by ELISA. Oxidative stress marker ROS was examined by fluorescence analysis. The NLRP3/IL-1β signaling pathway, Nrf2/HO-1 signaling pathway and tight junction proteins (ZO-1 and Occludin) were detected by RT-qPCR or Western blotting. In our research, it was observed that MA treatment effectively suppressed the notable increase in H₂O₂-induced inflammatory markers (TNF-α, IL-1β, and IL-18), decreased ROS accumulation, mitigated the expression of NLRP3, ASC, and caspase-1, and promoted the expression of ZO-1 and Occludin. After silencing the NLRP3 gene with siRNA, the protective influence of MAs was observed to be linked with the NLRP3 inflammasome. Additional investigations demonstrated that the treatment with MAs triggered the activation of Nrf2, facilitating its translocation into the nucleus. This process resulted in a notable upregulation of Nrf2, NQO1, and HO-1 expression, along with the initiation of the Nrf2-HO-1 signaling pathway. Consequently, there was an enhancement in the activities of antioxidant enzymes like SOD, GSH-Px, and CAT, which effectively mitigated the accumulation of ROS, thereby ameliorating the oxidative stress state. The antioxidant effectiveness of MAs was additionally heightened in the presence of SFN, an activator of Nrf2. The antioxidant and anti-inflammatory functions of MAs and their role in regulating intestinal epithelial tight junction protein disruption were significantly affected after siRNA knockdown of the Nrf2 gene. These findings suggest that MAs have the potential to reduce H₂O₂-triggered oxidative stress, inflammation, and disruption of intestinal epithelial tight junction proteins in IPEC-1 cells. This reduction is achieved by blocking the ROS/NLRP3/IL-1β signaling pathway through the activation of the Nrf2 pathway. Full article

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17 pages, 3268 KiB

Open AccessArticle

Species Composition and Distribution of Terrestrial Herbs in a High Montane Forest in Ecuador

by Catalina Quintana, Henrik Balslev and Renato Valencia

Diversity 2024, 16(5), 262; https://doi.org/10.3390/d16050262 (registering DOI) - 27 Apr 2024

Abstract

In mountain tropical forests, understory herbs have received little attention compared to trees, and their commonness and rarity are virtually unknown. We studied ground herbs to explore how they are assembled in a full one-hectare plot and to test the influence of light [...] Read more.

In mountain tropical forests, understory herbs have received little attention compared to trees, and their commonness and rarity are virtually unknown. We studied ground herbs to explore how they are assembled in a full one-hectare plot and to test the influence of light intensity (LI) and topographic habitats in species composition. The plot is a humid montane forest located in the Pasochoa Volcano, at 3300 m. We found 43 genera and 50 perennial species (30 angiosperms in 17 families, and 20 ferns). Interestingly, herbs are 64% richer in species than trees in the same study plot (50 vs. 32). Herbs were mostly obligately terrestrial (70% of the species), while 30% were fallen climbers and epiphytes rooted in the ground. Across the forest, there were 31,119 individuals that covered 8.5% of the ground. We concluded that both LI and topography shaped the species distribution, the floristic composition, and the community structure of ground herbs. For instance, 12% of the species were exclusively found in places with high LI; the rest of species grew in medium- to low-LI environments. Concerning rarity, we found that 39% of the species are rare (judging by botanical collections; <100), which stresses the need of conservation strategies for this group of plants. Full article

(This article belongs to the Special Issue Diversity Hotspots)

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17 pages, 3835 KiB

Open AccessArticle

Unveiling Lovastatin’s Anti-Inflammatory Potential in Mouse’s Brain during Acute Trypanosoma cruzi Infection

by Beatriz Matheus de Souza Gonzaga, Líndice Mitie Nisimura, Laura Lacerda Coelho, Roberto Rodrigues Ferreira, Samuel Iwao Maia Horita, Daniela Gois Beghini, Vanessa Estato, Tania Cremonini de Araújo-Jorge and Luciana Ribeiro Garzoni

Biology 2024, 13(5), 301; https://doi.org/10.3390/biology13050301 (registering DOI) - 27 Apr 2024

Abstract

Neurological commitment is a neglected manifestation of Chagas disease (CD). Meningoencephalitis mainly affects children and immunosuppressed patients, while stroke can occur with or without cardiac compromise. One of the possible causes of stroke development is microvascular commitment. Our group previously described that experimental [...] Read more.

Neurological commitment is a neglected manifestation of Chagas disease (CD). Meningoencephalitis mainly affects children and immunosuppressed patients, while stroke can occur with or without cardiac compromise. One of the possible causes of stroke development is microvascular commitment. Our group previously described that experimental Trypanossoma cruzi acute infection leads to cerebral microvasculopathy. This condition is characterized by decreased capillary density, increased leukocyte rolling and adhesion, and endothelial dysfunction. CD was discovered 114 years ago, and until today, only two drugs have been available for clinical treatment: benznidazole and nifurtimox. Both present a high cure rate for the acute phase (80%) and small cure rate for the chronic phase (20%). In addition, the high occurrence of side-effects, without proper medical follow-up, can result in treatment abandonment. Therefore, the search for new therapeutic schemes is necessary. Statins are drugs already used in the clinic that have several pleiotropic effects including endothelial function improvement, anti-inflammatory action, as well as trypanocidal effects, making them a potential alternative treatment for brain microvasculopathy in CD. Here, we investigate the effect of lovastatin (LOV) on brain microvasculopathy and inflammatory parameters. Swiss Webster mice were intraperitoneally inoculated with the Y strain of T. cruzi. Treatment with lovastatin (20 mg/kg/day) was initiated 24 h after the infection and continued for 14 consecutive days. We observed that LOV treatment did not affect parasitemia, brain microcirculation alterations, or the reduction in cerebral blood flow caused by T. cruzi infection. Also, LOV did not prevent the increased number of CD3+ cells and eNOS levels in the T. cruzi-infected brain. No alterations were observed on VCAM-1 and MCP-1 expressions, neither caused by infection nor LOV treatment. However, LOV prevented the increase in F4/80+ cells and ICAM-1 levels in the brain caused by acute infection with T. cruzi. These results suggest an anti-inflammatory activity of LOV, but more studies are needed to elucidate the role of LOV in CD acute infection. Full article

(This article belongs to the Special Issue Advances in Leishmaniasis and Chagas Disease: Biology, Epidemiology, Treatment and Control)

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12 pages, 283 KiB

Open AccessCommunication

Blood Cadmium Level Is a Marker of Cancer Risk in Men

by Róża Derkacz, Wojciech Marciniak, Piotr Baszuk, Monika Wysokińska, Natalia Chrzanowska, Marcin Lener, Tomasz Huzarski, Jacek Gronwald, Tadeusz Dębniak, Cezary Cybulski, Anna Jakubowska, Rodney J. Scott and Jan Lubiński

Nutrients 2024, 16(9), 1309; https://doi.org/10.3390/nu16091309 (registering DOI) - 27 Apr 2024

Abstract

Cadmium (Cd) is a known carcinogen, but its impact on cancer risk at lower concentrations is poorly understood. Previous studies on Cd and cancer risk in men show inconsistent results, prompting further investigation. A prospective cohort study involving 2956 men was conducted. Blood [...] Read more.

Cadmium (Cd) is a known carcinogen, but its impact on cancer risk at lower concentrations is poorly understood. Previous studies on Cd and cancer risk in men show inconsistent results, prompting further investigation. A prospective cohort study involving 2956 men was conducted. Blood Cd levels were measured, and participants were followed for 78 months to assess cancer incidence. Men with high blood Cd levels (>0.71 µg/L) had a significantly increased risk of cancer compared to those with low levels (<0.19 µg/L) (HR 3.42, p < 0.001), particularly among non-smokers (HR 3.74, p = 0.003), individuals aged < 60 years (HR 2.79, p = 0.017), and ≥60 (HR 4.63, p = 0.004). The influence of smoking on cancer risk based on Cd levels was not significant in this study. Blood Cd levels may influence cancer risk in men, emphasizing the importance of minimizing Cd exposure to reduce risk. Confirmation of these results in other populations is essential for effective preventive measures against Cd-related cancers. Full article

(This article belongs to the Special Issue The Role of Nutrition and Supplementation in Cancer Risk and Progression)

16 pages, 1126 KiB

Open AccessArticle

Illumina Sequencing and Metabolomic Analysis Explored the Effects of the Mixed Silage of Rice Straw and Chinese Cabbage Waste on Fecal Microorganisms and Metabolites in Hu Sheep

by Chuang Li, Zhiqi Lu, Ruxin Qi, Zhenbin Zhang, Yue Lu, Muhammad Hammad Zafar, Kailun Yang and Mengzhi Wang

Fermentation 2024, 10(5), 233; https://doi.org/10.3390/fermentation10050233 (registering DOI) - 26 Apr 2024

Abstract

Silage is the most important component of a ruminant diet and has important production and health significance in ruminant production. The aim of the research was to investigate how the mixed silage of Chinese cabbage waste and rice straw (mixed silage) impacts the [...] Read more.

Silage is the most important component of a ruminant diet and has important production and health significance in ruminant production. The aim of the research was to investigate how the mixed silage of Chinese cabbage waste and rice straw (mixed silage) impacts the fecal microorganisms and metabolites in Hu sheep using Illumina sequencing and metabolomic analysis. A total of 16 Hu sheep (8 rams and 8 ewes) weighing about 39 kg and 5.5 months old were used as experimental sheep and divided into two groups (4 rams and 4 ewes, n = 8) using the principle of randomized trials: the control group with peanut sprouts, corn husks, and sorghum husks as roughage and the silage group with the mixed silage as roughage. There were no significant differences in the average daily gain (ADG), dry matter intake (DMI), or feed conversion rate (FCR) between the control group and the mixed silage groups (p > 0.05). Microbiome results showed that 15 microorganisms such as Ruminococcaceae UCG 010, Breznakia, Erysipelothrix, Desulfovibrio, Succiniclasticum, and Shuttleworthia were significantly different between the two groups. In addition, metabolomics showed that the mixed silage modulated the concentrations and metabolic pathways of metabolites in the manure. Significantly different metabolites were mainly enriched in amino acid anabolism (“glycine, serine, and threonine metabolism”, “valine, leucine, and isoleucine biosynthesis”, “arginine biosynthesis”, etc.), nucleic acid metabolism (pyrimidine metabolism). In conclusion, the addition of mixed silage to the diet of Hu sheep can alter the structure of the hindgut microflora and regulate the metabolism of amino acids and nucleotides, which affects health performance. Full article

(This article belongs to the Special Issue Application of Fermentation Technology in Animal Nutrition)

14 pages, 2185 KiB

Open AccessArticle

Enhancing Escherichia coli Inactivation: Synergistic Mechanism of Ultraviolet Light and High-Voltage Electric Field

by Yihan Zhang, Yun Liang, Di Pan, Shupei Bai, Diya Wen, Min Tang, Hua Song, Xuan Guo and Hao Han

Foods 2024, 13(9), 1343; https://doi.org/10.3390/foods13091343 (registering DOI) - 26 Apr 2024

Abstract

This study investigated the bactericidal effects of ultraviolet (UV) radiation, a high-voltage electric field (HVEF), and their combination on Escherichia coli. The results indicated that UV and combined disinfection were more effective with longer exposure, leading to significant reductions in microbial activity. [...] Read more.

This study investigated the bactericidal effects of ultraviolet (UV) radiation, a high-voltage electric field (HVEF), and their combination on Escherichia coli. The results indicated that UV and combined disinfection were more effective with longer exposure, leading to significant reductions in microbial activity. Specifically, the single UV disinfection alone reduced activity by 3.3 log after 5 min, while combined disinfection achieved a 4.2 log reduction. In contrast, short-term HVEF treatment did not exhibit significant bactericidal effects, only achieving a reduction of 0.17 log in 5 min. Furthermore, prolonged exposure to both UV disinfection and an HVEF was found to damage cell membranes, ultimately causing cell death, while shorter durations did not. Despite rapid cell count decreases, flow cytometry did not detect apoptotic or necrotic cells, likely due to rapid cell rupture. This study suggests that combining UV radiation and an HVEF could be a promising approach for inhibiting bacterial reproduction, with HVEF enhancing UV effects. These findings provide insights for using combined HVEF and UV disinfection in food safety and preservation. Full article

(This article belongs to the Section Food Engineering and Technology)

15 pages, 1009 KiB

Open AccessArticle

The Association between Countermovement Rebound Jump Metrics and Independent Measures of Athletic Performance

by Jiaqing Xu, Anthony Turner, Thomas M. Comyns, Shyam Chavda and Chris Bishop

Appl. Sci. 2024, 14(9), 3718; https://doi.org/10.3390/app14093718 (registering DOI) - 26 Apr 2024

Abstract

This study investigates the associations between countermovement rebound jump (CMRJ) performance metrics and various independent measures of athletic performance, including the isometric mid-thigh pull (IMTP), 20 m linear sprint, and 505 change-of-direction (COD) speed tests. Pearson’s correlations were used to analyse the relationship [...] Read more.

This study investigates the associations between countermovement rebound jump (CMRJ) performance metrics and various independent measures of athletic performance, including the isometric mid-thigh pull (IMTP), 20 m linear sprint, and 505 change-of-direction (COD) speed tests. Pearson’s correlations were used to analyse the relationship between the CMRJ measures with athletic performance, with significance being set at p ≤ 0.006. Results showed large significant positive relationships between IMTP peak force and force at 300 milliseconds with the first jump height of the CMRJ (JH-1, r = 0.54 to 0.55, p ≤ 0.002). Additionally, inverse relationships were observed between reactive strength index modified (RSImod) and reactive strength index (RSI) with 20 m sprint total and split times (r = −0.55 to −0.66, p ≤ 0.001), and the 10 m and total sprint times were significantly correlated with JH-1 (r = −0.54, p = 0.003), indicating that greater vertical explosive power and reactive strength are associated with faster sprint performance. Finally, a significant inverse relationship was identified between CMRJ metrics (two JH values and RSImod) and 505 COD times in both the left and right sides (r = −0.51 to −0.68, p ≤ 0.006). These findings suggest that CMRJ performance metrics are valuable indicators of lower-limb explosive force production, with a strong link to both linear sprint and COD performance. The finding underscores the importance of including CMRJ assessments in athletic performance evaluations due to their dual assessment capacity of slow and fast stretch–shortening cycle mechanics. Full article

26 pages, 10430 KiB

Open AccessArticle

Emergency Strategies for Gushing Water of Borehole and Numerical Simulation on Circular Diaphragm Wall Excavation with Ring-Beams

by Yi-Hao Tsai, Chia-Feng Hsu, Kuo-Hsiang Ho and Shong-Loong Chen

Symmetry 2024, 16(5), 524; https://doi.org/10.3390/sym16050524 - 26 Apr 2024

Abstract

This study explores the underground structure and soil retention capabilities of a large-scale circular diaphragm wall (93.5 m in diameter) utilized as a soil retention strategy in deep excavation projects. The symmetrical design of the wall facilitates the use of an unsupported construction [...] Read more.

This study explores the underground structure and soil retention capabilities of a large-scale circular diaphragm wall (93.5 m in diameter) utilized as a soil retention strategy in deep excavation projects. The symmetrical design of the wall facilitates the use of an unsupported construction method, effectively resisting soil and water pressures. Using PLAXIS 3D 2017 software, this study simulates wall deformation and ground settlement, employing three different soil models to assess behavior under standard and emergency water gushing scenarios. The results show that the hardening soil (HS) model most accurately reflects the actual deformations and settlements. This study also finds that adjusting Young’s modulus for clay significantly impacts the accuracy of soil behavior predictions, while changes in the properties of sand have minimal effects. This research highlights the challenges posed by water gushing and suggests the need for model improvements to capture better the dynamic interactions between soil and water pressure, which could lead to wall tilting. Overall, this study offers innovative and practical value, providing crucial insights for designing and mitigating strategies in large-scale circular deep excavation projects, especially in regions such as Taiwan, where such constructions are rare and face unique challenges. Full article

(This article belongs to the Special Issue Symmetry, Finite Element Analysis, and Intelligent Sensing and Monitoring: Applications in Engineering)

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19 pages, 11008 KiB

Open AccessArticle

SAM-Induced Pseudo Fully Supervised Learning for Weakly Supervised Object Detection in Remote Sensing Images

by Xiaoliang Qian, Chenyang Lin, Zhiwu Chen and Wei Wang

Remote Sens. 2024, 16(9), 1532; https://doi.org/10.3390/rs16091532 - 26 Apr 2024

Abstract

Weakly supervised object detection (WSOD) in remote sensing images (RSIs) aims to detect high-value targets by solely utilizing image-level category labels; however, two problems have not been well addressed by existing methods. Firstly, the seed instances (SIs) are mined solely relying on the [...] Read more.

Weakly supervised object detection (WSOD) in remote sensing images (RSIs) aims to detect high-value targets by solely utilizing image-level category labels; however, two problems have not been well addressed by existing methods. Firstly, the seed instances (SIs) are mined solely relying on the category score (CS) of each proposal, which is inclined to concentrate on the most salient parts of the object; furthermore, they are unreliable because the robustness of the CS is not sufficient due to the fact that the inter-category similarity and intra-category diversity are more serious in RSIs. Secondly, the localization accuracy is limited by the proposals generated by the selective search or edge box algorithm. To address the first problem, a segment anything model (SAM)-induced seed instance-mining (SSIM) module is proposed, which mines the SIs according to the object quality score, which indicates the comprehensive characteristic of the category and the completeness of the object. To handle the second problem, a SAM-based pseudo-ground truth-mining (SPGTM) module is proposed to mine the pseudo-ground truth (PGT) instances, for which the localization is more accurate than traditional proposals by fully making use of the advantages of SAM, and the object-detection heads are trained by the PGT instances in a fully supervised manner. The ablation studies show the effectiveness of the SSIM and SPGTM modules. Comprehensive comparisons with 15 WSOD methods demonstrate the superiority of our method on two RSI datasets. Full article

(This article belongs to the Special Issue Object Detection and Information Extraction Based on Remote Sensing Imagery)

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21 pages, 1823 KiB

Open AccessArticle

Gadolinium Doping Modulates the Enzyme-like Activity and Radical-Scavenging Properties of CeO₂ Nanoparticles

by Madina M. Sozarukova, Taisiya O. Kozlova, Tatiana S. Beshkareva, Anton L. Popov, Danil D. Kolmanovich, Darya A. Vinnik, Olga S. Ivanova, Alexey V. Lukashin, Alexander E. Baranchikov and Vladimir K. Ivanov

Nanomaterials 2024, 14(9), 769; https://doi.org/10.3390/nano14090769 (registering DOI) - 26 Apr 2024

Abstract

Their unique physicochemical properties and multi-enzymatic activity make CeO₂ nanoparticles (CeO₂ NPs) the most promising active component of the next generation of theranostic drugs. When doped with gadolinium ions, CeO₂ NPs constitute a new type of contrast agent for magnetic [...] Read more.

Their unique physicochemical properties and multi-enzymatic activity make CeO₂ nanoparticles (CeO₂ NPs) the most promising active component of the next generation of theranostic drugs. When doped with gadolinium ions, CeO₂ NPs constitute a new type of contrast agent for magnetic resonance imaging, possessing improved biocatalytic properties and a high level of biocompatibility. The present study is focused on an in-depth analysis of the enzyme-like properties of gadolinium-doped CeO₂ NPs (CeO₂:Gd NPs) and their antioxidant activity against superoxide anion radicals, hydrogen peroxide, and alkylperoxyl radicals. Using an anion-exchange method, CeO₂:Gd NPs (~5 nm) with various Gd-doping levels (10 mol.% or 20 mol.%) were synthesized. The radical-scavenging properties and biomimetic activities (namely SOD- and peroxidase-like activities) of CeO₂:Gd NPs were assessed using a chemiluminescent method with selective chemical probes: luminol, lucigenin, and L-012 (a highly sensitive luminol analogue). In particular, gadolinium doping has been shown to enhance the radical-scavenging properties of CeO₂ NPs. Unexpectedly, both bare CeO₂ NPs and CeO₂:Gd NPs did not exhibit SOD-like activity, acting as pro-oxidants and contributing to the generation of reactive oxygen species. Gadolinium doping caused an increase in the pro-oxidant properties of nanoscale CeO₂. At the same time, CeO₂:Gd NPs did not significantly inhibit the intrinsic activity of the natural enzyme superoxide dismutase, and CeO₂:Gd NPs conjugated with SOD demonstrated SOD-like activity. In contrast to SOD-like properties, peroxidase-like activity was observed for both bare CeO₂ NPs and CeO₂:Gd NPs. This type of enzyme-like activity was found to be pH-dependent. In a neutral medium (pH = 7.4), nanoscale CeO₂ acted as a prooxidant enzyme (peroxidase), while in an alkaline medium (pH = 8.6), it lost its catalytic properties; thus, it cannot be regarded as a nanozyme. Both gadolinium doping and conjugation with a natural enzyme were shown to modulate the interaction of CeO₂ NPs with the key components of redox homeostasis. Full article

12 pages, 611 KiB

Open AccessArticle

Association of Polymorphisms in PD-1 and LAG-3 Genes with Acute Myeloid Leukemia

by Lamjed Mansour, Mashael Alqahtani, Ali Aljuaimlani, Jameel Al-Tamimi, Nouf Al-Harbi and Suliman Alomar

Medicina 2024, 60(5), 721; https://doi.org/10.3390/medicina60050721 - 26 Apr 2024

Abstract

Background and objectives: Acute myeloid leukemia (AML) is a hematological malignancy characterized by uncontrolled proliferation of immature myeloid cells. Immune checkpoint molecules such as programmed cell death protein 1 (PD-1) and lymphocyte activation gene-3 (LAG-3) are essential for controlling anti-tumor immune responses. This [...] Read more.

Background and objectives: Acute myeloid leukemia (AML) is a hematological malignancy characterized by uncontrolled proliferation of immature myeloid cells. Immune checkpoint molecules such as programmed cell death protein 1 (PD-1) and lymphocyte activation gene-3 (LAG-3) are essential for controlling anti-tumor immune responses. This study aims to explore the correlation between specific genetic variations (SNPs) in the PDCD1 (rs2227981) and LAG3 (rs12313899) genes and the likelihood of developing AML in the Saudi population. Material and methods: total of 98 Saudi AML patients and 131 healthy controls were genotyped for the PDCD1 rs2227981 and LAG3 rs12313899 polymorphisms using TaqMan genotyping assays. A logistic regression analysis was conducted to evaluate the relationship between the SNPs and AML risk using several genetic models. Results: The results revealed a significant association between the PDCD1 rs2227981 polymorphism and increased AML risk. In AML patients, the frequency of the G allele was considerably greater than in healthy controls (OR = 1.93, 95% CI: 1.31–2.81, p = 0.00080). The GG and AG genotypes were associated with a very high risk of developing AML (p < 0.0001). In contrast, no significant association was observed between the LAG3 rs12313899 polymorphism and AML risk in the studied population. In silico analysis of gene expression profiles from public databases suggested the potential impact of PDCD1 expression levels on the overall survival of AML patients. Conclusions: This study provides evidence for the association of the PDCD1 rs2227981 polymorphism with an increased risk for AML in the Saudi population. Full article

(This article belongs to the Section Genetics and Molecular Medicine)

8 pages, 2717 KiB

Open AccessArticle

Caesarean Section Scar and Placental Location at the First Trimester of Pregnancy—A Prospective Longitudinal Study

by Egle Savukyne, Mindaugas Kliucinskas, Laura Malakauskiene and Kristina Berskiene

Medicina 2024, 60(5), 719; https://doi.org/10.3390/medicina60050719 - 26 Apr 2024

Abstract

Background and Objectives: This study aims to report the location of the placenta in the first trimester of pregnancy in groups of women according to the number of previous caesarean deliveries and the visibility of the caesarean scar niche. Materials and Methods: [...] Read more.

Background and Objectives: This study aims to report the location of the placenta in the first trimester of pregnancy in groups of women according to the number of previous caesarean deliveries and the visibility of the caesarean scar niche. Materials and Methods: The prospective observational research included adult women aged 18 to 41 years during pregnancy after one or more previous caesarean sections (CSs). Transvaginal (TVS) and transabdominal sonography (TAS) was used to examine the uterine scar and placental location during 11–14 weeks. The CS scar niche (“defect”) was bordered in the sagittal plane as a notch at the previous CS scar’s site with a depth of 2.0 mm or more. A comparative analysis of the placental location (high or low and anterior or posterior) was performed between groups of women according to the CS number and the CS scar niche. Results: A total of 122 participants were enrolled during the first-trimester screening. The CS scar defect (“niche”) was visible in 40.2% of cases. In cases after one previous CS, the placenta was low in the uterine cavity (anterior or posterior) at 77.4%, and after two or more CSs, it was at 67.9%. Comparing the two groups according to the CS scar niche, the placenta was low in 75.5% of cases in the participant group with a CS scar niche and in 75% of cases without a CS scar niche (p = 0.949). Conclusions: The number of previous caesarean deliveries has no effect on the incidence rate of low-lying placentas in the first trimester. Moreover, the presence of the CS scar niche is not associated with anterior low-lying placentas. Full article

(This article belongs to the Section Obstetrics and Gynecology)

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12 pages, 1110 KiB

Open AccessCommunication

A New Full Digital Workflow for Fixed Prosthetic Rehabilitation of Full-Arch Edentulism Using the All-on-4 Concept

by João Martins, João Rangel, Miguel de Araújo Nobre, Ana Ferro, Mariana Nunes, Ricardo Almeida and Carlos Moura Guedes

Medicina 2024, 60(5), 720; https://doi.org/10.3390/medicina60050720 - 26 Apr 2024

Abstract

(1) Background: Recent digital workflows are being developed for full-arch rehabilitations supported by implants with immediate function. The purpose of this case series is to describe a new digital workflow for the All-on-4 concept. (2) Methods: The patients were rehabilitated using [...] Read more.

(1) Background: Recent digital workflows are being developed for full-arch rehabilitations supported by implants with immediate function. The purpose of this case series is to describe a new digital workflow for the All-on-4 concept. (2) Methods: The patients were rehabilitated using the All-on-4 concept with a digital workflow including computerized tomography scanning, intra-oral scanning, and CAD-CAM production of the temporary prosthesis, with the 3D printing of stackable guides (base guide, implant guide, and prosthetic guide). The passive fit of the prostheses and the time to perform the rehabilitations were evaluated. (3) Results: The digital workflow allowed for predictable bone reduction, the insertion of implants with immediate function, and the connection of an implant-supported prosthesis with immediate loading. The time registered to perform the full-arch rehabilitations (implant insertion, abutment connection, prosthesis connection) was below 2 hours and 30 min. No passive fit issues were noted. (4) Conclusions: within the limitation of this case series, the digital workflow applied to the All-on-4 concept using stackable base-, implant-, and prosthetic guides constitutes a potential alternative with decreased time for the procedure without prejudice of the outcome. Full article

(This article belongs to the Section Dentistry)

24 pages, 23162 KiB

Open AccessArticle

An Experimental Procedure to Study the High-Speed Orthogonal Cutting of Unidirectional GFRP

by Martina Panico, Luca Boccarusso, Antonio Formisano, Giuseppe Villani and Antonio Langella

J. Manuf. Mater. Process. 2024, 8(3), 87; https://doi.org/10.3390/jmmp8030087 - 26 Apr 2024

Abstract

The aim of this paper is to establish a valid procedure for better understanding all of the phenomena associated with the high-speed machining of glass fiber-reinforced plastic (GFRP) composites. Both rectangular and circular specimens were machined at high cutting speeds (up to 50 [...] Read more.

The aim of this paper is to establish a valid procedure for better understanding all of the phenomena associated with the high-speed machining of glass fiber-reinforced plastic (GFRP) composites. Both rectangular and circular specimens were machined at high cutting speeds (up to 50 m/min) in order to understand what occurred for all values of fiber orientation angles during machining operations. An innovative testing methodology was proposed and studied to investigate the phenomenon of burr formation and thus understand how to avoid it during machining operations. To this end, the forces arising during the machining process and the roughness of the resulting surface were carefully studied and correlated with the cutting angle. Additionally, the cutting surface and chip morphology formed during cutting tests were examined using a high-speed camera. Close correlations were found between the variations in the cutting forces’ signals and the trends of the surface roughness and the morphology of the machined surface. Full article

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12 pages, 750 KiB

Open AccessArticle

Pomegranate Juice Clarification Using Ultrafiltration: Influence of the Type of Variety and Degree of Ripeness

by Asunción M. Hidalgo, José A. Macario, Marta Abellán-Baeza, Teresa Sánchez-Moya, Rubén López-Nicolás and Fulgencio Marín-Iniesta

Separations 2024, 11(5), 134; https://doi.org/10.3390/separations11050134 - 26 Apr 2024

Abstract

Fruit consumption guarantees the supply of most of the necessary nutrients for a complete and balanced diet, as it is a relevant source of vitamins, minerals, and antioxidants. In particular, pomegranate has very interesting medicinal properties, such as an anti-inflammatory effect and the [...] Read more.

Fruit consumption guarantees the supply of most of the necessary nutrients for a complete and balanced diet, as it is a relevant source of vitamins, minerals, and antioxidants. In particular, pomegranate has very interesting medicinal properties, such as an anti-inflammatory effect and the protection of the cardiovascular system, among others. During pomegranate juice production, it appears cloudy and must be clarified to remove suspended solids such as colloids and high-molecular weight tannins. The membrane clarification process is a cost-effective alternative to the conventional methods, resulting in a high-quality product. In this work, the clarification of pomegranate juice using the Triple System Model F1 membrane module was carried out for the Mollar and Wonderful varieties with early and late maturity. Three ultrafiltration membranes with different molecular weight cut-off and different chemical compositions were used. The rejection coefficient and permeate flux (which represent the selectivity of the membranes and the process efficiency, respectively) were measured. GR-40PP showed the best results in terms of membrane selectivity and process efficiency, achieving adequate physicochemical juice parameters. Regarding the comparison of the maturity degree, in general terms, the Mollar variety showed better results. Ripe pomegranates showed greater selectivity, while the process efficiency was higher for the early samples. Full article

(This article belongs to the Section Analysis of Natural Products and Pharmaceuticals)

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21 pages, 623 KiB

Open AccessReview

Phthalates: The Main Issue in Quality Control in the Beverage Industry

by Alessia Iannone, Cristina Di Fiore, Fabiana Carriera, Pasquale Avino and Virgilio Stillittano

Separations 2024, 11(5), 133; https://doi.org/10.3390/separations11050133 - 26 Apr 2024

Abstract

Phthalate esters (PAEs) are a group of chemicals used to improve the flexibility and durability of plastics. The chemical properties and the resistance to high temperatures promote their degradation and release into the environment. Food and beverages can be contaminated by PAEs through [...] Read more.

Phthalate esters (PAEs) are a group of chemicals used to improve the flexibility and durability of plastics. The chemical properties and the resistance to high temperatures promote their degradation and release into the environment. Food and beverages can be contaminated by PAEs through the migration from packaging material because they are not covalently bound to plastic and also via different kinds of environmental sources or during processing. For instance, alcoholic drinks in plastic containers are a particular risk, since the ethanol contained provides a good solubility for PAEs. According to its role as an endocrine disruptor compound and its adverse effects on the liver, kidney, and reproductive and respiratory systems, the International Agency on Research Cancer (IARC) classified di-(2-ethylhexyl) phthalate (DEHP) as a possible human carcinogen. For this reason, to control human exposure to PAEs, many countries prohibited their use in food as non-food substances. For example, in Europe, the Commission Regulation (EU) 2018/2005 restricts the use of DEHP, dibutyl phthalate (DBP), benzyl butyl phthalate (BBP), and diisobutyl phthalate (DiBP) to a concentration equal to or below 0.1 by weight in plasticizers in articles used by consumers or in indoor areas. There are reports from the US Food and Drug Administration (FDA) that some beverages (and food as well), particularly fruit juices, contain high levels of phthalates. In some cases, the deliberate adulteration of soft drinks with phthalate esters has been reported. This paper would like to show the difficulties of performing PAE analysis in beverage matrices, in particular alcoholic beverages, as well as the main solutions provided for quality control in the industrial branches. Full article

(This article belongs to the Section Analysis of Food and Beverages)

18 pages, 1391 KiB

Open AccessArticle

Dynamic Changes of Environment and Gut Microbial Community of Litopenaeus vannamei in Greenhouse Farming and Potential Mechanism of Gut Microbial Community Construction

by Hui Li, Shuwen Gu, Libao Wang, Wenjun Shi, Qi Jiang and Xihe Wan

Fishes 2024, 9(5), 155; https://doi.org/10.3390/fishes9050155 - 26 Apr 2024

Abstract

The aim of this study was to investigate the dynamic changes in the microbial communities of both the environment and gut of Litopenaeus vannamei, as well as to elucidate the mechanisms underlying microbial community assembly in greenhouse farming. 16S rDNA high-throughput sequencing [...] Read more.

The aim of this study was to investigate the dynamic changes in the microbial communities of both the environment and gut of Litopenaeus vannamei, as well as to elucidate the mechanisms underlying microbial community assembly in greenhouse farming. 16S rDNA high-throughput sequencing and bioinformatics methods were used to carry out the research on the community structure of the microorganisms under greenhouse culture conditions in water, sediment, and gut microorganisms; correlations pertaining to environmental factors; the feasibility of using Source Tracker; and the mechanisms of community construction. The results show that the dominant microorganisms in water, sediment, and gut farming in a greenhouse environment varied and were subject to dynamic change. A variety of beneficial microbiota such as Bacillus were found in the gut, whereas a variety of microorganisms such as Marivita and Pseudomonas, which function as nitrogen and phosphorus removers, were present in water. Source Tracker and environmental correlation analyses showed that changes in the gut were associated with eutrophication indicators (total nitrogen, total phosphorus, ammonia nitrogen) and changes in environmental microorganisms (in water and sediment). The results of the community-building mechanism analysis show that stochastic processes determine the community-building directions of environmental and gut microorganisms. These findings will help us to understand the microbiota characteristics of shrimp ponds under greenhouse farming conditions, and the complex interactions between the shrimp gut and the environmental microbiota and environmental variables, as well as revealing the changing rules of the gut microbiota. Full article

(This article belongs to the Special Issue Aquaculture Ecology and the Environmental Microbiome)

15 pages, 3619 KiB

Open AccessArticle

A Multi-Modal Foundation Model to Assist People with Blindness and Low Vision in Environmental Interaction

by Yu Hao, Fan Yang, Hao Huang, Shuaihang Yuan, Sundeep Rangan, John-Ross Rizzo, Yao Wang and Yi Fang

J. Imaging 2024, 10(5), 103; https://doi.org/10.3390/jimaging10050103 - 26 Apr 2024

Abstract

People with blindness and low vision (pBLV) encounter substantial challenges when it comes to comprehensive scene recognition and precise object identification in unfamiliar environments. Additionally, due to the vision loss, pBLV have difficulty in accessing and identifying potential tripping hazards independently. Previous assistive [...] Read more.

People with blindness and low vision (pBLV) encounter substantial challenges when it comes to comprehensive scene recognition and precise object identification in unfamiliar environments. Additionally, due to the vision loss, pBLV have difficulty in accessing and identifying potential tripping hazards independently. Previous assistive technologies for the visually impaired often struggle in real-world scenarios due to the need for constant training and lack of robustness, which limits their effectiveness, especially in dynamic and unfamiliar environments, where accurate and efficient perception is crucial. Therefore, we frame our research question in this paper as: How can we assist pBLV in recognizing scenes, identifying objects, and detecting potential tripping hazards in unfamiliar environments, where existing assistive technologies often falter due to their lack of robustness? We hypothesize that by leveraging large pretrained foundation models and prompt engineering, we can create a system that effectively addresses the challenges faced by pBLV in unfamiliar environments. Motivated by the prevalence of large pretrained foundation models, particularly in assistive robotics applications, due to their accurate perception and robust contextual understanding in real-world scenarios induced by extensive pretraining, we present a pioneering approach that leverages foundation models to enhance visual perception for pBLV, offering detailed and comprehensive descriptions of the surrounding environment and providing warnings about potential risks. Specifically, our method begins by leveraging a large-image tagging model (i.e., Recognize Anything Model (RAM)) to identify all common objects present in the captured images. The recognition results and user query are then integrated into a prompt, tailored specifically for pBLV, using prompt engineering. By combining the prompt and input image, a vision-language foundation model (i.e., InstructBLIP) generates detailed and comprehensive descriptions of the environment and identifies potential risks in the environment by analyzing environmental objects and scenic landmarks, relevant to the prompt. We evaluate our approach through experiments conducted on both indoor and outdoor datasets. Our results demonstrate that our method can recognize objects accurately and provide insightful descriptions and analysis of the environment for pBLV. Full article

(This article belongs to the Special Issue Image and Video Processing for Blind and Visually Impaired)

14 pages, 552 KiB

Open AccessArticle

Preparation of Magnetic Nano-Catalyst Containing Schiff Base Unit and Its Application in the Chemical Fixation of CO₂ into Cyclic Carbonates

by Na Kang, Yindi Fan, Dan Li, Xiaoli Jia and Sanhu Zhao

Magnetochemistry 2024, 10(5), 33; https://doi.org/10.3390/magnetochemistry10050033 - 26 Apr 2024

Abstract

The development of a catalyst for the conversion of CO₂ and epoxides to the corresponding cyclic carbonates is still a very attractive topic. Magnetic nano-catalysts are widely used in various organic reactions due to their magnetic separation and recycling properties. Here, a [...] Read more.

The development of a catalyst for the conversion of CO₂ and epoxides to the corresponding cyclic carbonates is still a very attractive topic. Magnetic nano-catalysts are widely used in various organic reactions due to their magnetic separation and recycling properties. Here, a magnetic nano-catalyst containing a Schiff base unit was designed, synthesized and used as a heterogeneous catalyst to catalyze CO₂and epoxides to form cyclic carbonates without solvents and co-catalysts. The catalyst was characterized using Fourier transform infrared (FTIR), X-ray diffraction (XRD), thermogravimetric (TG), VSM, SEM, TEM and BET. The results show that the magnetic nano-catalyst containing the Schiff base unit has a high activity in the solvent-free cycloaddition reaction of CO₂ with epoxide under mild conditions, and is easily separated from the reaction mixture driven by external magnetic force. The recovered catalyst maintains a high performance after five cycles. Full article

15 pages, 620 KiB

Open AccessArticle

The Influence of Reinforced Fibers and Opacifiers on the Effective Thermal Conductivity of Silica Aerogels

by Binghuan Huang, Jingbei Li, Liang Gong, Pengcheng Dai and Chuanyong Zhu

Gels 2024, 10(5), 300; https://doi.org/10.3390/gels10050300 - 26 Apr 2024

Abstract

Fiber–particle-reinforced silica aerogels are widely applied in thermal insulation. Knowing their effective thermal conductivity (ETC) and radiative characteristics under high temperatures is necessary to improve their performance. This article first analyzes the radiation characteristics of silica aerogels doped with opacifier particles and reinforced [...] Read more.

Fiber–particle-reinforced silica aerogels are widely applied in thermal insulation. Knowing their effective thermal conductivity (ETC) and radiative characteristics under high temperatures is necessary to improve their performance. This article first analyzes the radiation characteristics of silica aerogels doped with opacifier particles and reinforced fibers, and then a universal model is established to predict the ETC. Furthermore, the impacts of different parameters of opacifier particles and reinforced fibers on the thermal insulation performance of silica aerogels are investigated. The results indicate that SiC exhibits comparatively strong absorption characteristics, making it a good alternative for opacifiers to improve thermal insulation performance under high temperatures. For the given type and volume fraction of opacifier particles, there exists an optimal diameter and volume fraction to achieve the best insulation performance of silica aerogel under a certain temperature. Considering that SiO₂ fibers exhibit a limited extinction capability and higher conductive thermal conductivity under high temperatures, for fiber–particle-reinforced silica aerogels, it is beneficial for their insulation performance to reduce the fiber volume fraction when the required mechanical properties are satisfied. Full article

(This article belongs to the Special Issue Recent Advances in Aerogels and Aerogel Composites)

13 pages, 5095 KiB

Open AccessArticle

The Putative Cytochrome b5 Domain-Containing Protein CaDap1 Homologue Is Involved in Antifungal Drug Tolerance, Cell Wall Chitin Maintenance, and Virulence in Candida albicans

by Dayong Xu, Manman Wang, Xing Zhang, Hongchen Mao, Haitao Xu, Biao Zhang, Xin Zeng and Feng Li

J. Fungi 2024, 10(5), 316; https://doi.org/10.3390/jof10050316 - 26 Apr 2024

Abstract

Candida albicans (Ca), a prominent opportunistic fungal pathogen in humans, has garnered considerable attention due to its infectious properties. Herein, we have identified and characterized CaCDAP1 (Ca orf19.1034), a homolog of ScDAP1 found in Saccharomyces cerevisiae. [...] Read more.

Candida albicans (Ca), a prominent opportunistic fungal pathogen in humans, has garnered considerable attention due to its infectious properties. Herein, we have identified and characterized CaCDAP1 (Ca orf19.1034), a homolog of ScDAP1 found in Saccharomyces cerevisiae. CaCDAP1 encodes a 183-amino acid protein with a conserved cytochrome b5-like heme-binding domain. The deletion of CaDAP1 renders Ca cells susceptible to caspofungin and terbinafine. CaDAP1 deletion confers resistance to Congo Red and Calcofluor White, and sensitivity to sodium dodecyl sulfate. The deletion of CaDAP1 results in a 50% reduction in chitin content within the cell wall, the downregulation of phosphorylation levels in CaMkc1, and the upregulation of phosphorylation levels in CaCek1. Notably, CaDAP1 deletion results in the abnormal hyphal development of Ca cells and diminishes virulence in a mouse systemic infection model. Thus, CaDAP1 emerges as a critical regulator governing cellular responses to antifungal drugs, the synthesis of cell wall chitin, and virulence in Ca. Full article

(This article belongs to the Section Fungal Pathogenesis and Disease Control)

12 pages, 412 KiB

Open AccessFeature PaperArticle

The Development of a Multienzyme Isothermal Rapid Amplification Assay to Visually Detect Duck Hepatitis B Virus

by Shuqi Xu, Yuanzhuo Man, Xin Xu, Jun Ji, Yan Wang, Lunguang Yao, Qingmei Xie and Yingzuo Bi

Vet. Sci. 2024, 11(5), 191; https://doi.org/10.3390/vetsci11050191 - 26 Apr 2024

Abstract

Duck hepatitis B virus (DHBV) is widely prevalent in global ducks and has been identified in Chinese geese with a high prevalence; the available detection techniques are time-consuming and require sophisticated equipment. In this study, an assay combining multienzyme isothermal rapid amplification (MIRA) [...] Read more.

Duck hepatitis B virus (DHBV) is widely prevalent in global ducks and has been identified in Chinese geese with a high prevalence; the available detection techniques are time-consuming and require sophisticated equipment. In this study, an assay combining multienzyme isothermal rapid amplification (MIRA) and lateral flow dipstick (LFD) was developed for the efficient and rapid detection of DHBV. The primary reaction condition of the MIRA assay for DHBV detection was 10 min at 38 °C without a temperature cycler. Combined with the LFD assay, the complete procedure of the newly developed MIRA assay for DHBV detection required only 15 min, which is about one-fourth of the reaction time for routine polymerase chain reaction assay. And electrophoresis and gel imaging equipment were not required for detection and to read the results. Furthermore, the detection limit of MIRA was 45.6 copies per reaction, which is approximately 10 times lower than that of a routine polymerase chain reaction assay. The primer set and probe had much simpler designs than loop-mediated isothermal amplification, and they were only specific to DHBV, with no cross-reactivity with duck hepatitis A virus subtype 1 and duck hepatitis A virus subtype 3, goose parvovirus, duck enteritis virus, duck circovirus, or Riemerella anatipestifer. In this study, we offer a simple, fast, and accurate assay method to identify DHBV in clinical serum samples of ducks and geese, which would be suitable for widespread application in field clinics. Full article

(This article belongs to the Special Issue Diagnosis and Evolution Analysis of Virus Infection in Poultry and Wild Birds)

10 pages, 289 KiB

Open AccessData Descriptor

Training Datasets for Epilepsy Analysis: Preprocessing and Feature Extraction from Electroencephalography Time Series

by Christian Riccio, Angelo Martone, Gaetano Zazzaro and Luigi Pavone

Data 2024, 9(5), 61; https://doi.org/10.3390/data9050061 - 26 Apr 2024

Abstract

We describe 20 datasets derived through signal filtering and feature extraction steps applied to the raw time series EEG data of 20 epileptic patients, as well as the methods we used to derive them. Background: Epilepsy is a complex neurological disorder which has [...] Read more.

We describe 20 datasets derived through signal filtering and feature extraction steps applied to the raw time series EEG data of 20 epileptic patients, as well as the methods we used to derive them. Background: Epilepsy is a complex neurological disorder which has seizures as its hallmark. Electroencephalography plays a crucial role in epilepsy assessment, offering insights into the brain’s electrical activity and advancing our understanding of seizures. The availability of tagged training sets covering all seizure phases—inter-ictal, pre-ictal, ictal, and post-ictal—is crucial for data-driven epilepsy analyses. Methods: Using the sliding window technique with a two-second window length and a one-second time slip, we extract multiple features from the preprocessed EEG time series of 20 patients from the Freiburg Seizure Prediction Database. In addition, we assign a class label to each instance to specify its corresponding seizure phase. All these operations are made through a software application we developed, which is named Training Builder. Results: The 20 tagged training datasets each contain 1080 univariate and bivariate features, and are openly and publicly available. Conclusions: The datasets support the training of data-driven models for seizure detection, prediction, and clustering, based on features engineering. Full article

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