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Anti-proliferative and Pro-apoptotic Effect of Dichloromethane Extract of Octopus vulgaris By-Products on Human Breast Cancer Cell Lines

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Abstract

Purpose

Octopus by-products are considered as a waste and no data of the activity of their lipid fractions have ever been reported. The aim of this study was to valorize Octopus vulgaris by-products by investigating their dichloromethane extract effect on proliferation and apoptosis of human breast cancer cell lines.

Methods

Two lipidics subfractions, F3336 and F3740, of Octopus by-products were obtained by silica gel chromatography after total lipid extraction. Their effects on proliferation, migration and apoptosis were examined on MCF-7 and MDA-MB-231 human breast cancer cell lines. The 3-(4,5-dimethyl-2-thiazol)-2,5 diphenyltetrazolium bromides (MTT) assay was used for the cell viability. Cell death was determined by flow cytometric analysis after 7-aminoactinomycin D (7-AAD) staining. MDA-MB-231 invasion and migration were analyzed using the Boyden chamber and wound healing assay.

Results

Treatment with the two lipidics subfractions showed a reduction in the proliferation of MCF-7 and MDA-MB-231 cell lines in a dose dependent manner. Treatments significantly inhibited MCF-7 cells growth than MDA-MB 231 cells. Moreover, the two lipidics subfractions induced apoptosis of MCF-7 cells and reduced invasion and migration of MDA-MB 231 cells.

Conclusion

This study shows for the first time that O. vulgaris lipid extracts have an antiproliferative and apoptotic effects on human breast cancer cell lines.

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References

  1. The State of World Fisheries and Aquaculture. Food and Agriculture Organization, United Nations, Rome (2014). www.fao.org/3/a-i3720e

  2. Chamras, H., Ardashian, A., Heber, D., Glaspy, J.A.: Fatty acid modulation of MCF-7 human breast cancer cell proliferation, apoptosis and differentiation. J. Nutr. Biochem. 13, 711–716 (2002)

    Article  Google Scholar 

  3. Sakakima, Y., Hayakawa, A., Nagasaka, T., Nakao, A.: Prevention of hepatocarcinogenesis with phosphatidylcholine and menaquinone-4: in vitro and in vivo experiments. J. Hepatol. 47, 83–92 (2007)

    Article  Google Scholar 

  4. Byju, K., Anuradha, V., Vasundhara, G., Nair, S.M., Kumar, N.C.: In vitro and in silico studies on the anticancer and apoptosis-inducing activities of the sterols identified from the soft coral, subergorgia reticulata. Pharmacogn. Mag. 10, 65–71 (2014)

    Article  Google Scholar 

  5. Mendilaharsu, M., De Stefani, E., Deneo-Pellegrini, H., Carzoglio, J., Ronco, A.: Phytosterols and risk of lung cancer: a case–control study in Uruguay. Lung Cancer 21, 37–45 (1998)

    Article  Google Scholar 

  6. De Stefani, E., Boffetta, P., Ronco, A.L., Brennan, P., Deneo-Pellegrini, H., Carzoglio, J.C., Mendilaharsu, M.: Plant sterols and risk of stomach cancer: a case–control study in Uruguay. Nutr. Cancer 37, 140–144 (2000)

    Article  Google Scholar 

  7. McCann, S.E., Freudenheim, J.L., Marshall, J.R., Graham, S.: Risk of human ovarian cancer is related to dietary intake of selected nutrients, phytochemicals and food groups. J. Nutr. 133, 1937–1942 (2003)

    Google Scholar 

  8. Ju, Y.H., Clausen, L.M., Allred, K.F., Almada, A.L., Helferich, W.G.: β-Sitosterol, β-sitosterol glucoside, and a mixture of β-sitosterol and β-sitosterol glucoside modulate the growth of estrogen-responsive breast cancer cells in vitro and in ovarioectomized athymic mice. J. Nutr. 134, 1145–1151 (2004)

    Google Scholar 

  9. El Roz, A., Bard, J.M., Huvelin, J.M., Nazih, H.: LXR agonists and ABCG1-dependent cholesterol efflux in MCF-7 breast cancer cells: relation to proliferation and apoptosis. Anticancer Res. 32, 3007–3013 (2012)

    Google Scholar 

  10. El Roz, A., Bard, J.M., Huvelin, J.M., Nazih, H.: The anti-proliferative and pro-apoptotic effects of the trans9, trans11 conjugated linoleic acid isomer on MCF-7 breast cancer cells are associated with LXR activation. Prostaglandins Leukot. Essent. Fat. Acids 88, 265–272 (2012)

    Article  Google Scholar 

  11. Lu, X., Liu, J., Hou, F., Liu, Z., Cao, X., Seo, H., Gao, B.: Cholesterol induces pancreatic β cell apoptosis through oxidative stress pathway. Cell Stress Chaperones 16, 539–548 (2011)

    Article  Google Scholar 

  12. Folch, J., Lees, M., Sloane-Stanley, G.H.: A simple method for the isolation and purification of total lipids from animal tissues. J. Biol. Chem. 226, 497–509 (1957)

    Google Scholar 

  13. Hinton, A., Sennoune, R.S., Bond, S., Fang, M., Reuveni, M.G., Sahagian, G., Jay, D., Martinez-Zaguilan, R., Forgac, M.: Function of a subunit isoforms of the V-ATPase in pH homeostasis and in vitro invasion of MDA-MB231 human breast cancer cells. J. Biol. Chem. 284, 16400–16408 (2009)

    Article  Google Scholar 

  14. Sugarbaker, E.V.: The complex clinical model of metastasis. Med. Biol. 2, 235–278 (1981)

    Google Scholar 

  15. Tsujino, T., Yoshida, K., Nakayama, H., Ito, H., Shimosato, T., Tahara, R.: Alterations of oncogenes in metastatic tumors of human gastric carcinomas. Br. J. Cancer 62, 226–230 (1990)

    Article  Google Scholar 

  16. Pasquet, V., Morisset, P., Ihammouine, S., Chepied, A., Aumailley, L., Berard, J.-B., Serive, B., Kaas, R., Lanneluc, I., Thiery, V., Lafferriere, M., Piot, J.-M., Patrice, T., Cadoret, J.-P., Picot, L.: Antiproliferative activity of violaxanthin isolated from bioguided fractionation of dunaliella tertiolecta extracts. Mar. Drugs 9, 819–831 (2011)

    Article  Google Scholar 

  17. Motta, L.B., Furlan, C.M., Santos, D.Y.A.C., Salatino, M.L.F., Duarte-Almeida, J.M., Negri, G.E., De Carvalho, J.E., Ruiz, A.L.T.G., Cordeiro, I., Salatino, A.: Constituents and antiproliferative activity of extracts from leaves of Croton macrobothrys. Braz. J. Pharmacogn. 21, 972–977 (2011)

    Article  Google Scholar 

  18. Taghizadeh, R.S.Z., Mahmoudi, M., Ahi, A., Emami, S.A.: Anti-proliferative effects of extracts from iranian artemisia species on cancer cell lines. Pharm. Biol. 49, 962–969 (2011)

    Article  Google Scholar 

  19. Bardonemail, S., Foussard, V., Fournel, S., Loubat, A.: Monoterpenes inhibit proliferation of human colon cancer cells by modulating cell cycle-related protein expression. Cancer Lett. 181, 187–194 (2002)

    Article  Google Scholar 

  20. Hong, J.-Y., Nam, J.-W., Seo, E.-K., Lee, S.K.: Daphnane diterpene esters with anti-proliferative activities against human lung cancer cells from Daphne genkwa. Chem. Pharm. Bull. 58, 234–237 (2010)

    Article  Google Scholar 

  21. Paduch, R., Kandefer-Szerszeń, M., Trytek, M., Fiedurek, J.: Terpenes: substances useful in human healthcare. Arch. Immunol. Ther. Exp. J. 55, 315–327 (2007)

    Article  Google Scholar 

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Acknowledgments

We wish to thank Chloé Chaillou for her technical help. This study was supported by the Bourse du Gouvernement Français (BGF) and the grants from the Western Indian Ocean Marine Science Association (WIOMSA) into the Marine Research Grant (MARG II).

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Authors and Affiliations

  1. Institut Halieutique et des Sciences Marines, University of Toliara, Toliara, Madagascar

    Edda Miray Fitahia, Christian Edmond Raheriniaina & Eulalie Ranaivoson

  2. EA-2160, MMS “Mer, Molécules, Santé”, Faculty of Pharmacy, University of Nantes, Nantes, France

    Jean-Michel Huvelin & Hassan Nazih

  3. EA 1155, Cibles et Médicaments des Infections de l’immunité et du Cancer, Faculty of Pharmacy, University of Nantes, Nantes, France

    Marc Antoine Bazin & Cédric Logé

  4. MMS EA2160, 1 Rue Gaston Veil, Laboratoire de Biochimie, UFR des Sciences Pharmaceutiques et Biologiques, 44068, Nantes, France

    Hassan Nazih

Authors
  1. Edda Miray Fitahia
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  2. Christian Edmond Raheriniaina
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  3. Marc Antoine Bazin
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  4. Jean-Michel Huvelin
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  5. Cédric Logé
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  6. Eulalie Ranaivoson
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  7. Hassan Nazih
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Correspondence to Hassan Nazih.

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Fitahia, E.M., Raheriniaina, C.E., Bazin, M.A. et al. Anti-proliferative and Pro-apoptotic Effect of Dichloromethane Extract of Octopus vulgaris By-Products on Human Breast Cancer Cell Lines. Waste Biomass Valor 6, 237–242 (2015). https://doi.org/10.1007/s12649-014-9344-1

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  • DOI: https://doi.org/10.1007/s12649-014-9344-1

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