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Preparation and characterization of artificial skin using chitosan and gelatin composites for potential biomedical application

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Abstract

A bioadhesive wound-dressing material based on the combination of gelatin and chitosan with a proper ratio was developed and successfully applied in biomedical fields. The composite films were prepared with increase in chitosan concentration in a fixed amount of gelatin and were evaluated for mechanical stability (e.g., tensile strength, elongation-at-break), water and buffer uptake capacity, water and buffer aging, molecular structure, morphology, thermal stability, and for biological properties (e.g., antimicrobial activity, cytotoxicity, in vivo wound-healing performance). It is noteworthy that the 10:3 (gelatin:chitosan) composite films showed the best physico-mechanical, thermal, and antimicrobial properties among the other ratios blend films. The improved mechanical and thermal stability of the 10:3 composite film suggested its promising use as carrier for controlled release drug. The composite film was evaluated using a rat model for in vivo tests to ascertain the applicability of the proper ratio of the chitosan and gelatin in the film for best wound-healing activity. Wound sites dresses with gelatin/chitosan composite films showed excellent rapid healing of the wound surface than those dressed with eco-plaster and gauze. Within a day after dressing with 10:3 composite film, the healing efficiency was found to be 80 %.

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Acknowledgments

The authors thank the staff of Radiation and Polymer Chemistry Laboratory, Institute of Nuclear Science and Technology, Bangladesh Atomic Energy Commission for technical support and advice throughout the work.

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Authors and Affiliations

  1. Department of Applied Chemistry and Chemical Engineering, University of Dhaka, Dhaka, Bangladesh

    Shahed Parvez & M. Mizanur Rahman

  2. Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh

    Mubarak A. Khan, Jahid M. M. Islam & Mostak Ahmed

  3. Department of Chemistry, Shahjalal University of Science and Technology, Sylhet, Bangladesh

    M. Anwar H. Khan & Belal Ahmed

  4. Department of Chemistry, Jagannath University, Dhaka, Bangladesh

    M. Fizur Rahman

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  1. Shahed Parvez
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  2. M. Mizanur Rahman
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  3. Mubarak A. Khan
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  4. M. Anwar H. Khan
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  5. Jahid M. M. Islam
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  6. Mostak Ahmed
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  7. M. Fizur Rahman
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  8. Belal Ahmed
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Correspondence to Mubarak A. Khan.

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Parvez, S., Rahman, M.M., Khan, M.A. et al. Preparation and characterization of artificial skin using chitosan and gelatin composites for potential biomedical application. Polym. Bull. 69, 715–731 (2012). https://doi.org/10.1007/s00289-012-0761-7

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  • DOI: https://doi.org/10.1007/s00289-012-0761-7

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