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Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering

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Abstract

Four tubular membranes based on PLA and gelatin with different arrangements (PLA, gelatin, PLA-gelatin Blend and PLA/gelatin Core/Shell) were prepared by the electrospinning technique, the gelatin within the materials was crosslinked with genipin and all the meshes were characterized by SEM, ATR-FTIR, TGA, DSC, XRD and mechanical tests, also a viability essay and a confocal microscopy of HUVECs in contact with the materials were carried out. A 50% decrement of cellular viability was observed in the fibers with Core/Shell structure since the first day of culture compared with the tissue culture polystyrene, due to solubilization of non-crosslinked gelatin.

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Authors and Affiliations

  1. Departamento de Investigación en Polímeros y Materiales, Universidad de Sonora, 83000, Hermosillo Sonora, Mexico

    Abraham Alejandro Leyva-Verduzco, María Mónica Castillo-Ortega, José Carmelo Encinas-Encinas, Teresa Del Castillo-Castro, Dora Evelia Rodríguez-Félix & Hisila del Carmen Santacruz-Ortega

  2. Departamento de Física, Universidad de Sonora, 83000, Hermosillo Sonora, Mexico

    Lerma Hanaiy Chan-Chan

  3. Departamento de Investigación en Física, Universidad de Sonora, 83000, Hermosillo Sonora, Mexico

    Erika Silva-Campa

  4. GSE Biomedical, 83150, Hermosillo Sonora, Mexico

    Ramsés Galaz-Méndez

  5. Instituto de Investigaciones en Materiales, Universidad Nacional Autónoma de México, 04510, Ciudad de México, Mexico

    Ricardo Vera-Graziano

  6. Centro de Investigación y de Estudios Avanzados Unidad Querétaro, 76230, Santiago de Querétaro, Querétaro, Mexico

    Irela Santos-Sauceda

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  1. Abraham Alejandro Leyva-Verduzco
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Leyva-Verduzco, A.A., Castillo-Ortega, M.M., Chan-Chan, L.H. et al. Electrospun tubes based on PLA, gelatin and genipin in different arrangements for blood vessel tissue engineering. Polym. Bull. 77, 5985–6003 (2020). https://doi.org/10.1007/s00289-019-03057-7

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