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Dissolved extracellular polymeric substances (dEPS) dynamics and bacterial growth during sea ice formation in an ice tank study

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Abstract

Extracellular polymeric substances (EPS) are known to help microorganisms to survive under extreme conditions in sea ice. High concentrations of EPS are reported in sea ice from both poles; however, production and dynamics of EPS during sea ice formation have been little studied to date. This investigation followed the production and partitioning of existing and newly formed dissolved organic matter (DOM) including dissolved carbohydrates (dCHO), dissolved uronic acids (dUA) and dissolved EPS (dEPS), along with bacterial abundances during early stages of ice formation. Sea ice was formed from North Sea water with (A) ambient DOM (NSW) and (B) with additional algal-derived DOM (ADOM) in a 6d experiment in replicated mesocosms. In ADOM seawater, total bacterial numbers (TBN) increased throughout the experiment, whereas bacterial growth occurred for 5d only in the NSW seawater. TBN progressively decreased within developing sea ice but with a 2-fold greater decline in NSW compared to ADOM ice. There were significant increases in the concentrations of dCHO in ice. Percentage contribution of dEPS was highest (63%) in the colder, uppermost parts in ADOM ice suggesting the development of a cold-adapted community, producing dEPS possibly for cryo-protection and/or protection from high salinity brines. We conclude that in the early stages of ice formation, allochthonous organic matter was incorporated from parent seawater into sea ice and that once ice formation had established, there were significant changes in the concentrations and composition of dissolved organic carbon pool, resulting mainly from the production of autochthonous DOM by the bacteria.

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Acknowledgments

The work described in this report was supported by the European Community’s Sixth Framework Programme through the grant to the budget of the Integrated Infrastructure Initiative HYDRALAB III, Contract no. 022441(RII3) and U.K. Natural Environment Research Council (NE/E016251/1). The authors would like to thank the Hamburg Ship Model Basin (HSVA), especially Kalle Evers and the ice tank crew, for the hospitality, technical and scientific support and the professional execution of the test programme in the Research Infrastructure ARCTECLAB. We are indebted to Naomi Thomas for the unenviable task of producing the ADOM additive and her support in nutrient and DOM analyses. Erika Allhusen provided essential support for the setting up and successful execution of the experiment. We thank Dr Ben Green for PRIMER analysis. We are very grateful to 3 anonymous reviewers for their detailed and highly constructive suggestions on an earlier draft of the manuscript.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, Essex, CO4 3SQ, UK

    Shazia N. Aslam & Graham J. C. Underwood

  2. Finnish Environment Institute (SYKE), Marine Research Centre, P.O. Box 140, 00251, Helsinki, Finland

    Hermanni Kaartokallio, Riitta Autio & David N. Thomas

  3. Ocean Sciences, College of Natural Science, Bangor University, Menai Bridge, Anglesey, LL59 5AB, UK

    Louiza Norman & David N. Thomas

  4. Alfred Wegener Institute for Polar and Marine Research, Am Handelshafen 12, 27570, Bremerhaven, Germany

    Michael Fischer & Gerhard S. Dieckmann

  5. Tvärminne Zoological Station, University of Helsinki, Helsinki, Finland

    Harri Kuosa

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  1. Shazia N. Aslam
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Correspondence to Shazia N. Aslam.

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Aslam, S.N., Underwood, G.J.C., Kaartokallio, H. et al. Dissolved extracellular polymeric substances (dEPS) dynamics and bacterial growth during sea ice formation in an ice tank study. Polar Biol 35, 661–676 (2012). https://doi.org/10.1007/s00300-011-1112-0

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