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Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves

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Abstract

Low temperature is one of the most severe environmental factors that impair plant growth and agricultural production. To investigate how Thellungiella halophila, an Arabidopsis-like extremophile, adapts to cold stress, a comparative proteomic approach based on two-dimensional electrophoresis was adopted to identify proteins that changed in abundance in Thellungiella rosette leaves during short term (6 h, 2 and 5 days) and long term (24 days) exposure to cold stress. Sixty-six protein spots exhibited significant change at least at one time point and maximal cold stress induced-proteome change was found in long-term cold stress group while the minimal change was found in 6-h cold treatment group. Fifty protein spots were identified by mass spectrometry analysis. The identified proteins mainly participate in photosynthesis, RNA metabolism, defense response, energy pathway, protein synthesis, folding and degradation, cell wall and cytoskeleton and signal transduction. These proteins might work cooperatively to establish a new homeostasis under cold stress. Nearly half of the identified cold-responsive proteins were associated with various aspects of chloroplast physiology suggesting that the cold stress tolerance of T. halophila is achieved, at least partly, by regulation of chloroplast function. All protein spots involved in RNA metabolism, defense response, protein synthesis, folding and degradation were found to be upregulated markedly by cold treatment, indicating enhanced RNA metabolism, defense and protein metabolism may play crucial roles in cold tolerance mechanism in T. halophila.

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Abbreviations

CBB:

Coomassie brilliant blue

ESI–MS/MS:

Tandem electrospray ionization-mass spectrometry

IEF:

Isoelectric focusing

LC–MS/MS:

Liquid chromatography–tandem mass spectrometry

MALDI-TOF MS:

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry

RT-PCR:

Reverse transcription-polymerase chain reaction

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Acknowledgments

This work was supported by the National Basic Research Program of China (No. 2006CB100100, 2004CB117307), the National Natural Science Foundation of China (No. 30670203, 30570434), National Key Project for Transgenic Organisms Breeding (No. 2009ZX08009-059B), MUC-985 and open fund of Key Laboratory of Cell Proliferation and Regulation Biology, Ministry of Education of China.

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Authors and Affiliations

  1. Key Laboratory for Cell Proliferation and Regulation Biology of Ministry of Education, College of Life Science, Beijing Normal University, 100875, Beijing, China

    Fei Gao, Yijun Zhou, Weiping Zhu, Xiaofeng Li & Genfa Zhang

  2. College of Life and Environmental Sciences, Minzu University of China, 100081, Beijing, China

    Fei Gao & Yijun Zhou

  3. Peking-Yale Joint Center for Plant Molecular Genetics and Agro-Biotechnology, National Laboratory of Protein Engineering and Plant Genetic Engineering, College of Life Sciences, Peking University, 100871, Beijing, China

    Liumin Fan

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  1. Fei Gao
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  2. Yijun Zhou
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  4. Xiaofeng Li
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Correspondence to Genfa Zhang.

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Gao, F., Zhou, Y., Zhu, W. et al. Proteomic analysis of cold stress-responsive proteins in Thellungiella rosette leaves. Planta 230, 1033–1046 (2009). https://doi.org/10.1007/s00425-009-1003-6

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