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Dynamic response of the transcriptome of a psychrophilic diatom, Chaetoceros neogracile, to high irradiance

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Abstract

Large-scale RNA profiling revealed that high irradiance differentially regulated 577 out of 1,439 non-redundant genes of the Antarctic marine diatom Chaetoceros neogracile, represented on a custom cDNA chip, during 6 h of treatment. Among genes that were up- or down-regulated more than twofold within 30 min of treatment (310/1,439), about half displayed an acclimatory response during 6 h under high light. Expression of the remaining non-acclimatory genes also rapidly returned to initial levels within 30 min following a shift to low irradiance. High light altered expression of most of the photosynthesis genes (48/70), in contrast to genes in other functional categories. In addition, opposite response patterns were provoked in genes encoding fucoxanthin chlorophyll a/c binding protein (FCP), the main component of the diatom light-harvesting complex; high irradiance caused a decrease in expression of most FCP genes, but drove the rapid and specific up-regulation of ten others. C. neogracile responded very promptly to a change in light intensity by rapidly adjusting the transcript levels of FCP genes up-regulated by high light, and these dynamic adjustments coincided well with diatoxanthin (Dtx) levels formed by the xanthophyll cycle under the same conditions. The observation that the non-photochemical quenching (NPQ) capacity of this polar diatom was highly dependent on Dtx, which could bind to FCP and trigger NPQ, suggests that the up-regulated FCP gene products may participate in a photoprotective process as Dtx-binding proteins.

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Abbreviations

DTT:

Dithiothreitol

NPQ:

Non-photochemical quenching

Ddx:

Diadinoxanthin

Dtx:

Diatoxanthin

DDE:

Diadinoxanthin de-epoxidase

FCP:

Fucoxanthin chlorophyll a/c binding protein

LHC:

Light-harvesting complex

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Acknowledgments

This work was supported by the Research Fund of Hanyang University (HY/2006/S).

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Authors and Affiliations

  1. Department of Life Science, College of Natural Science, Hanyang University, Seoul, 133-791, Republic of Korea

    Seunghye Park, Gyeongseo Jung & EonSeon Jin

  2. Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, Republic of Korea

    Yong-sic Hwang

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  1. Seunghye Park
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  2. Gyeongseo Jung
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Correspondence to Yong-sic Hwang or EonSeon Jin.

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S. Park and G. Jung have equally contributed to the paper.

E. Jin and Y. Hwang are authors to whom correspondence should be addressed.

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Park, S., Jung, G., Hwang, Ys. et al. Dynamic response of the transcriptome of a psychrophilic diatom, Chaetoceros neogracile, to high irradiance. Planta 231, 349–360 (2010). https://doi.org/10.1007/s00425-009-1044-x

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