Abstract
The low molecular weight RNA components of maize have been analyzed after labeling callus and leaf tissue with [3H]uridine in vitro. Electrophoresis of the isolated RNA on acrylamide slab gels reveals, apart from 5S and transfer RNA, three major and about five minor RNA species with chain lengths between 140 and 280 nucleotides. These RNA molecules are labeled as rapidly as 5S, transfer RNA, and do not represent degradation products of large ribosomal RNA molecules. Furthermore, like 5S and transfer RNA, these small RNA species are stable and show no detectable turnover within forty-eight hours. Fractionation of the tissue into crude subcellular fractions indicates a preferential association of some of the small stable RNA species with the nucleus, while others appear to be located in the cytoplasm. The low molecular weight RNA spectrum from the leaf is similar to that observed in callus, with the major small RNA species equally present in both tissues.
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Abbreviations
- tRNA:
-
transfer RNA
- hnRNA:
-
heterogenous nuclear RNA
- mRNA:
-
messenger RNA
- scRNA:
-
small cytoplasmic RNA
- snRNA:
-
small nuclear RNA
References
Benecke, B.J., Penman, S. (1977) A new class of small nuclear RNA molecules synthesized by a type I RNA polymerase in HeLa cells. Cell 12, 939–946
Benecke, B.J., Ben-Zeèv, A., Penman, S. (1980) The regulation of RNA metabolism in suspended and rearrached anchorage-dependent 3T6 fibroblasts. J. Cell. Physiol. 103, in press
Deimel, B., Louis, C., Sekeris, C.E. (1977) The presence of small molecular weight RNAs in nuclear ribonucleoprotein particles carrying hnRNA. FEBS Lett. 73, 80–94
Eliceiri, G. (1974) Short-lived small RNAs in the cytoplasm of HeLa cells. Cell 3, 11–14
Frederiksen S., Pederson, I.R., Hellung-Larsen, P., Engberg, J. (1974) Metabolic studies of small molecular weight nuclear RNA components in BHK-21 cells. Biochim. Biophys. Acta 340, 64–76
Goldstein, L., Ko, C (1974) Electrophoretic characterization of shuttling and nonshuttling snRNAs. Cell 2, 259–266
Green, C.E., Phillips, R.L. (1976) Plant regeneration from tissue cultures of maize. Crop Sci. 15, 417–421
Hellung-Larsen, P., Frederiksen, S., Plessner, P. (1971) Small molecular weight RNA components in Tetrahymena pyriformis. Biochim. Biophys. Acta 254, 78–90
Kanai, R., Edwards, G.E. (1973) Separation of mesophyll protoplasts and bundle sheath cells from maize leaves for photosynthetic studies. Plant Physiol. 52, 484–490
Lerner, M.R., Boyle, J.A., Mount, S.M., Wolin, S.L., Steitz, J.A. (1980) Are snRNPs involved in splicing?. Nature (London) 283, 220–224
McReynolds, L., Penman, S. (1974) A polymerase activity forming 5S and pre-4S RNA in isolated HeLa cell nuclei. Cell 1, 139–145
Mennes, A.M., Bouman, H., van den Burg, H.P.M., Libbenga, K.R. (1978) RNA synthesis in isolated tobacco callus nuclei, and the influence of phytohormones. Plant Sci. Lett. 13, 329–339
Reichel, R., Benecke, B.J. (1980) Reinitiation of synthesis of small cytoplasmic RNA species K and L in isolated HeLa cell nuclei in vitro. Nucl. Acids Res. 8, 225–234
Ro-Choi, T.S., Busch, H. (1974) Low molecular weight nuclear RNA. In: The cell nucleus, pt. 3, pp. 151–208, Busch, H. ed. Academic Press, New York
Seifart, K.H., Benecke, B.J., Juhasz, P.P. (1972) Multiple RNA polymerase species from rat liver tissue: Possible existence of a cytoplasmic enzyme. Arch. Biochem. Biophys. 151, 515–531
Udvardy, A., Seifart, K.H. (1976) Transcription of specific genes in isolated nuclei from HeLa cells in vitro. Eur. J. Biochem. 62, 353–363
Weinberg, R., Penman, S. (1968) Small molecular weight monodisperse nuclear RNA. J. Mol. Biol. 38, 289–304
Weinmann, R., Roeder, R.G. (1974) Role of DNA-dependent polymerase III in the transcription of the tRNA and 5S RNA genes. Proc. Natl. Acad. Sci. USA 71, 1790–1794
Whitfeld, P.R., Leaver, C.J., Bottomley, W., Atchison, B.A. (1978) Low-molecular (4.5S) ribonucleic acid in higher-plant chloroplast ribosomes. Biochem. J. 175, 1103–1112
Zieve, G., Penman, S. (1976) Small RNA species of the HeLa cell: Metabolism and subcellular localization. Cell 8, 19–31
Zieve, G., Benecke, B.J., Penman, S. (1977) Synthesis of two classes of small RNA species in vivo and in vitro. Biochemistry 16, 4520–4525
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Link, G., Benecke, BJ. Small RNA species in maize tissue. Planta 150, 406–411 (1980). https://doi.org/10.1007/BF00390177
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DOI: https://doi.org/10.1007/BF00390177