Skip to main content
Log in

Calmodulin-like protein from the fern Anemia phyllitidis L. Sw.

  • Published:
Planta Aims and scope Submit manuscript

Abstract

Spores and prothallia of the fern Anemia phyllitidis L. Sw. contain a protein which in its physicochemical properties corresponds largely to calmodulin. It shows immunoreactivity with a calmodulin antiserum and activates bovine brain phosphodiesterase. Its content increases during the early processes of light-induced spore germination, indicating that the Ca2+-dependence of these processes may be mediated by this protein.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

Abbreviations

EGTA:

ethylene, glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

RIA:

radioimmunoassay

References

  • Burgess, W.H., Jemiolo, D.K., Kretsinger, R.H. (1980) Interaction of calcium and calmodulin in the presence of SDS. Biochim. Biophys. Acta 623, 257–270

    Google Scholar 

  • Burgess, W.H., Schleicher, M., van Eldik, L.J., Watterson, D.M. (1983) Comparative studies of calmodulin. In: Calcium and cell function, vol. 4, pp. 209–261, Cheung, W.Y., ed. Academic Press, New York

    Google Scholar 

  • Caldwell, Ch.R., Haug, A. (1981) Affinity chromatographic isolation of calmodulin from bovine-brain acetone powder. Anal. Biochem. 116, 325–330

    Google Scholar 

  • Cheung, W.Y. (1971) Cyclic 3′ 5′-nucleotide phosphodiesterase. Evidence for and properties of a protein activator. J. Biol. Chem. 246, 2859–2869

    Google Scholar 

  • Dieter, P. (1984) Calmodulin and calmodulin mediated processes in plants. Plant Cell Environ. 7, 371–380

    Google Scholar 

  • Fry, I.J., Villa, L., Kuehn, G.D., Hageman, J.H. (1986) Calmodulin-like protein from Bacillus subtilis. Biochem. Biophys. Res. Commun. 134, 212–217

    Google Scholar 

  • Gopalakrishna, R., Anderson, W.B. (1982) Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-sepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104, 830–836

    Google Scholar 

  • Haupt, W. (1985) Effects of nutrients and light pretreatment on phytochrome-mediated fern-spore germination. Planta 164, 63–68

    Google Scholar 

  • Inovye, S., Harada, W., Zusman, D., Inouye, M. (1981) Development-specific proteins of Myxococcus xanthus: Purification and characterization. J. Bacteriol. 148, 678–683

    Google Scholar 

  • Iwasa, Y., Yonemitsu, K., Matsui, K., Fukunaga, K., Miyamoto, E. (1981) Calmodulin-like activity in the soluble fraction of Escherichia coli. Biochem. Biophys. Res. Commun. 98, 656–660

    Google Scholar 

  • Kakiucki, S., Yamazaki, R. (1970) Calcium-dependent phosphodiesterase activity and its activating factor (PAF) from brain. Biochem. Biophys. Res. Commun. 41, 1104–1110

    Google Scholar 

  • Klee, C.B., Vanaman, T.C. (1982) Calmodulin. Adv. Protein Chem. 35, 213–321

    Google Scholar 

  • Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of Bacteriophage T4. Nature 277, 680–685

    Google Scholar 

  • Mohr, H. (1956) Die Abhängigkeit des Protonemawachstums und der Protonemapolarität bei Farnen vom Licht. Planta 47, 127–158

    Google Scholar 

  • Saunders, M.J., Hepler, P.K. (1983) Calcium antagonists and calmodulin inhibitors block cytokinin-induced bud formation in Funaria. Dev. Biol. 99, 41–49

    Google Scholar 

  • Wagner, G., Valentin, P., Dieter, P. Marmél, D. (1984) Identification of calmodulin in the green alga Mougcotia and its possible function in chloroplast reorientational movement. Planta 162, 62–67

    Google Scholar 

  • Watterson, D.M., Iverson, D.B., van Eldik, L.J. (1980a) Rapid separation and quantitation of 3′ 5′-cyclic nucleotides and 5′ nucleotides in phosphodiesterase reaction mixtures using high-performance liquid chromatography. J. Biochem. Biophys. Methods 2, 139–146

    Google Scholar 

  • Watterson, D.M., Iverson, D.B., van Eldik, L.J. (1980b) Spinach calmodulin: Isolation, characterization, and comparison with vertebrate calmodulins. Biochemistry 19, 5762–5768

    Google Scholar 

  • Wayne, R., Hepler, P.K. (1984) The role of calcium ions in phytochrome-mediated germination of spores of Onoclea sensibilis. Planta 160, 12–20

    Google Scholar 

  • Wayne, R., Hepler, P.K. (1985) Red light stimulates an increase in intracellular calcium in the spores of Onoclea sensibilis. Plant Physiol. 77, 8–11

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Föhr, K.J., Enßle, M. & Schraudolf, H. Calmodulin-like protein from the fern Anemia phyllitidis L. Sw.. Planta 171, 127–129 (1987). https://doi.org/10.1007/BF00395075

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00395075

Key words

Navigation