Abstract
Previously, we established experimental conditions allowing us to induce hypomethylation of tandem arrays of highly repetitive DNA sequences in the Nicotiana tabacum L. nuclear genome (M. Bezděk et al. 1991, Planta 184, 487–490). In this paper, we demonstrate that loci containing the highly repetitive sequences of the HRS60 family can maintain the induced hypomethylated state through protoplast regeneration, non-differentiated callus growth, and plant regeneration. The hypomethylation, induced with 5-azacytidine and monitored on these sequences, did not substantially alter the capacity of calli to produce plants. It can be concluded that, in contradistinction of multiple copies of transgenes or ectopic genes which are usually recognized as methylation targets, endogenous tandem repeats, such as the HRS60, present at 105 copies in the genome, can escape de-novo methylation.
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Abbreviations
- AzaC:
-
5-azacytidine
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This project was supported by the Grant Agency of the Academy of Sciences of the Czech Republic. We thank Ms. Libuše Jedličková, Ms. Hana Suchánková, and Ms. Emilie Koudelková for technical assistance.
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Koukalová, B., Kuhrová, V., Vyskot, B. et al. Maintenance of the induced hypomethylated state of tobacco nuclear repetitive DNA sequences in the course of protoplast and plant regeneration. Planta 194, 306–310 (1994). https://doi.org/10.1007/BF00197529
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DOI: https://doi.org/10.1007/BF00197529